Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1519-6984.262851 http://hdl.handle.net/11449/236906 |
Resumo: | Nontuberculous mycobacteria infection is one of the most common chronic bacterial diseases in ornamental aquarium fish and appears to be directly related to stressful husbandry practices. Furthermore, it also represents zoonotic potential. Here we present the isolation and characterization of non-tuberculous mycobacteria from diseased freshwater angelfish (Pterophyllum scalare) in São Paulo, Brazil. Nine discarded breeding females with signs of disease were evaluated. The fish exhibited lethargy, loss of appetite, cachexia, skin ulcers, and exophthalmia. At necropsy, four fishes presented macroscopic granulomas in the spleen. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare and M. peregrinum were isolated and identified by hsp65 PCR restriction analysis. Histopathological analysis revealed microscopic lesions compatible with mycobacteriosis, and Mycobacterium bacillus were observed by Ziehl-Neelsen stain. Notably, all Mycobacterium species identified in this study have already been reported in human patients; therefore, diseased animals may be a source of infection for people who handle fish and aquariums. |
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Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquariumIsolamento de Mycobacterium spp. potencialmente zoonótica de peixes anjo de água doce (Pterophyllum scalare) doentes de um aquárioMycobacteriumfreshwater angelfishgranulomaPCRMycobacteriumpeixe-anjo de água docegranulomaPCRNontuberculous mycobacteria infection is one of the most common chronic bacterial diseases in ornamental aquarium fish and appears to be directly related to stressful husbandry practices. Furthermore, it also represents zoonotic potential. Here we present the isolation and characterization of non-tuberculous mycobacteria from diseased freshwater angelfish (Pterophyllum scalare) in São Paulo, Brazil. Nine discarded breeding females with signs of disease were evaluated. The fish exhibited lethargy, loss of appetite, cachexia, skin ulcers, and exophthalmia. At necropsy, four fishes presented macroscopic granulomas in the spleen. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare and M. peregrinum were isolated and identified by hsp65 PCR restriction analysis. Histopathological analysis revealed microscopic lesions compatible with mycobacteriosis, and Mycobacterium bacillus were observed by Ziehl-Neelsen stain. Notably, all Mycobacterium species identified in this study have already been reported in human patients; therefore, diseased animals may be a source of infection for people who handle fish and aquariums.A infecção por micobactérias não tuberculosas é uma das doenças bacterianas crônicas mais comuns em peixes ornamentais de aquário e parece estar diretamente relacionada a práticas de manejo estressantes. Além disso, também representa potencial zoonótico. Aqui, apresentamos o isolamento e a caracterização de micobactérias não tuberculosas de peixes-anjo de água doce doentes (Pterophyllum scalare) em São Paulo, Brasil. Foram avaliadas nove fêmeas reprodutoras descartadas com sinais de doença. Os peixes exibiam letargia, perda de apetite, caquexia, úlceras cutâneas e exoftalmia. Na necropsia, quatro peixes apresentaram granulomas macroscópicos no baço. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare e M. peregrinum foram isolados e identificados por análise de restrição por PCR de hsp65. A análise histopatológica revelou lesões microscópicas compatíveis com micobacteriose, e o bacilo de Mycobacterium foi observado pela coloração de Ziehl-Neelsen. Notavelmente, todas as espécies de Mycobacterium identificadas neste estudo já foram relatadas em pacientes humanos; portanto, animais doentes podem ser uma fonte de infecção para pessoas que manipulam peixes e aquários.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade de São Paulo, Departamento de Medicina Veterinária Preventiva e Saúde AnimalUniversidade Estadual Paulista (UNESP), Instituto de BiotecnologiaUniversidade Estadual Paulista (UNESP), Instituto de BiotecnologiaCAPES: 001CAPES: 1808006CNPq: 154900/2018-4CNPq: 310736/2018-8Instituto Internacional de EcologiaUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Cardoso, P. H. M.Moreno, L. Z.Ikuta, C. Y.Rodrigues, M. V. [UNESP]Donola, S. T.Heinemann, M. B.Balian, S. C.Moreno, A. M.2022-10-10T13:56:09Z2022-10-10T13:56:09Z2022-06-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1519-6984.262851Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022.1519-69841678-4375http://hdl.handle.net/11449/23690610.1590/1519-6984.262851S1519-69842024000100325S1519-69842024000100325.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biologyinfo:eu-repo/semantics/openAccess2024-01-11T06:27:24Zoai:repositorio.unesp.br:11449/236906Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:47.896373Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium Isolamento de Mycobacterium spp. potencialmente zoonótica de peixes anjo de água doce (Pterophyllum scalare) doentes de um aquário |
title |
Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium |
spellingShingle |
Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium Cardoso, P. H. M. Mycobacterium freshwater angelfish granuloma PCR Mycobacterium peixe-anjo de água doce granuloma PCR |
title_short |
Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium |
title_full |
Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium |
title_fullStr |
Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium |
title_full_unstemmed |
Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium |
title_sort |
Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium |
author |
Cardoso, P. H. M. |
author_facet |
Cardoso, P. H. M. Moreno, L. Z. Ikuta, C. Y. Rodrigues, M. V. [UNESP] Donola, S. T. Heinemann, M. B. Balian, S. C. Moreno, A. M. |
author_role |
author |
author2 |
Moreno, L. Z. Ikuta, C. Y. Rodrigues, M. V. [UNESP] Donola, S. T. Heinemann, M. B. Balian, S. C. Moreno, A. M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Cardoso, P. H. M. Moreno, L. Z. Ikuta, C. Y. Rodrigues, M. V. [UNESP] Donola, S. T. Heinemann, M. B. Balian, S. C. Moreno, A. M. |
dc.subject.por.fl_str_mv |
Mycobacterium freshwater angelfish granuloma PCR Mycobacterium peixe-anjo de água doce granuloma PCR |
topic |
Mycobacterium freshwater angelfish granuloma PCR Mycobacterium peixe-anjo de água doce granuloma PCR |
description |
Nontuberculous mycobacteria infection is one of the most common chronic bacterial diseases in ornamental aquarium fish and appears to be directly related to stressful husbandry practices. Furthermore, it also represents zoonotic potential. Here we present the isolation and characterization of non-tuberculous mycobacteria from diseased freshwater angelfish (Pterophyllum scalare) in São Paulo, Brazil. Nine discarded breeding females with signs of disease were evaluated. The fish exhibited lethargy, loss of appetite, cachexia, skin ulcers, and exophthalmia. At necropsy, four fishes presented macroscopic granulomas in the spleen. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare and M. peregrinum were isolated and identified by hsp65 PCR restriction analysis. Histopathological analysis revealed microscopic lesions compatible with mycobacteriosis, and Mycobacterium bacillus were observed by Ziehl-Neelsen stain. Notably, all Mycobacterium species identified in this study have already been reported in human patients; therefore, diseased animals may be a source of infection for people who handle fish and aquariums. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-10T13:56:09Z 2022-10-10T13:56:09Z 2022-06-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1519-6984.262851 Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022. 1519-6984 1678-4375 http://hdl.handle.net/11449/236906 10.1590/1519-6984.262851 S1519-69842024000100325 S1519-69842024000100325.pdf |
url |
http://dx.doi.org/10.1590/1519-6984.262851 http://hdl.handle.net/11449/236906 |
identifier_str_mv |
Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022. 1519-6984 1678-4375 10.1590/1519-6984.262851 S1519-69842024000100325 S1519-69842024000100325.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129450203676672 |