Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium

Detalhes bibliográficos
Autor(a) principal: Cardoso, P. H. M.
Data de Publicação: 2022
Outros Autores: Moreno, L. Z., Ikuta, C. Y., Rodrigues, M. V. [UNESP], Donola, S. T., Heinemann, M. B., Balian, S. C., Moreno, A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1519-6984.262851
http://hdl.handle.net/11449/236906
Resumo: Nontuberculous mycobacteria infection is one of the most common chronic bacterial diseases in ornamental aquarium fish and appears to be directly related to stressful husbandry practices. Furthermore, it also represents zoonotic potential. Here we present the isolation and characterization of non-tuberculous mycobacteria from diseased freshwater angelfish (Pterophyllum scalare) in São Paulo, Brazil. Nine discarded breeding females with signs of disease were evaluated. The fish exhibited lethargy, loss of appetite, cachexia, skin ulcers, and exophthalmia. At necropsy, four fishes presented macroscopic granulomas in the spleen. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare and M. peregrinum were isolated and identified by hsp65 PCR restriction analysis. Histopathological analysis revealed microscopic lesions compatible with mycobacteriosis, and Mycobacterium bacillus were observed by Ziehl-Neelsen stain. Notably, all Mycobacterium species identified in this study have already been reported in human patients; therefore, diseased animals may be a source of infection for people who handle fish and aquariums.
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spelling Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquariumIsolamento de Mycobacterium spp. potencialmente zoonótica de peixes anjo de água doce (Pterophyllum scalare) doentes de um aquárioMycobacteriumfreshwater angelfishgranulomaPCRMycobacteriumpeixe-anjo de água docegranulomaPCRNontuberculous mycobacteria infection is one of the most common chronic bacterial diseases in ornamental aquarium fish and appears to be directly related to stressful husbandry practices. Furthermore, it also represents zoonotic potential. Here we present the isolation and characterization of non-tuberculous mycobacteria from diseased freshwater angelfish (Pterophyllum scalare) in São Paulo, Brazil. Nine discarded breeding females with signs of disease were evaluated. The fish exhibited lethargy, loss of appetite, cachexia, skin ulcers, and exophthalmia. At necropsy, four fishes presented macroscopic granulomas in the spleen. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare and M. peregrinum were isolated and identified by hsp65 PCR restriction analysis. Histopathological analysis revealed microscopic lesions compatible with mycobacteriosis, and Mycobacterium bacillus were observed by Ziehl-Neelsen stain. Notably, all Mycobacterium species identified in this study have already been reported in human patients; therefore, diseased animals may be a source of infection for people who handle fish and aquariums.A infecção por micobactérias não tuberculosas é uma das doenças bacterianas crônicas mais comuns em peixes ornamentais de aquário e parece estar diretamente relacionada a práticas de manejo estressantes. Além disso, também representa potencial zoonótico. Aqui, apresentamos o isolamento e a caracterização de micobactérias não tuberculosas de peixes-anjo de água doce doentes (Pterophyllum scalare) em São Paulo, Brasil. Foram avaliadas nove fêmeas reprodutoras descartadas com sinais de doença. Os peixes exibiam letargia, perda de apetite, caquexia, úlceras cutâneas e exoftalmia. Na necropsia, quatro peixes apresentaram granulomas macroscópicos no baço. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare e M. peregrinum foram isolados e identificados por análise de restrição por PCR de hsp65. A análise histopatológica revelou lesões microscópicas compatíveis com micobacteriose, e o bacilo de Mycobacterium foi observado pela coloração de Ziehl-Neelsen. Notavelmente, todas as espécies de Mycobacterium identificadas neste estudo já foram relatadas em pacientes humanos; portanto, animais doentes podem ser uma fonte de infecção para pessoas que manipulam peixes e aquários.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade de São Paulo, Departamento de Medicina Veterinária Preventiva e Saúde AnimalUniversidade Estadual Paulista (UNESP), Instituto de BiotecnologiaUniversidade Estadual Paulista (UNESP), Instituto de BiotecnologiaCAPES: 001CAPES: 1808006CNPq: 154900/2018-4CNPq: 310736/2018-8Instituto Internacional de EcologiaUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Cardoso, P. H. M.Moreno, L. Z.Ikuta, C. Y.Rodrigues, M. V. [UNESP]Donola, S. T.Heinemann, M. B.Balian, S. C.Moreno, A. M.2022-10-10T13:56:09Z2022-10-10T13:56:09Z2022-06-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1519-6984.262851Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022.1519-69841678-4375http://hdl.handle.net/11449/23690610.1590/1519-6984.262851S1519-69842024000100325S1519-69842024000100325.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biologyinfo:eu-repo/semantics/openAccess2024-01-11T06:27:24Zoai:repositorio.unesp.br:11449/236906Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:47.896373Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
Isolamento de Mycobacterium spp. potencialmente zoonótica de peixes anjo de água doce (Pterophyllum scalare) doentes de um aquário
title Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
spellingShingle Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
Cardoso, P. H. M.
Mycobacterium
freshwater angelfish
granuloma
PCR
Mycobacterium
peixe-anjo de água doce
granuloma
PCR
title_short Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
title_full Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
title_fullStr Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
title_full_unstemmed Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
title_sort Isolation of potential zoonotic Mycobacterium spp. from diseased freshwater angelfish (Pterophyllum scalare) from an aquarium
author Cardoso, P. H. M.
author_facet Cardoso, P. H. M.
Moreno, L. Z.
Ikuta, C. Y.
Rodrigues, M. V. [UNESP]
Donola, S. T.
Heinemann, M. B.
Balian, S. C.
Moreno, A. M.
author_role author
author2 Moreno, L. Z.
Ikuta, C. Y.
Rodrigues, M. V. [UNESP]
Donola, S. T.
Heinemann, M. B.
Balian, S. C.
Moreno, A. M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Cardoso, P. H. M.
Moreno, L. Z.
Ikuta, C. Y.
Rodrigues, M. V. [UNESP]
Donola, S. T.
Heinemann, M. B.
Balian, S. C.
Moreno, A. M.
dc.subject.por.fl_str_mv Mycobacterium
freshwater angelfish
granuloma
PCR
Mycobacterium
peixe-anjo de água doce
granuloma
PCR
topic Mycobacterium
freshwater angelfish
granuloma
PCR
Mycobacterium
peixe-anjo de água doce
granuloma
PCR
description Nontuberculous mycobacteria infection is one of the most common chronic bacterial diseases in ornamental aquarium fish and appears to be directly related to stressful husbandry practices. Furthermore, it also represents zoonotic potential. Here we present the isolation and characterization of non-tuberculous mycobacteria from diseased freshwater angelfish (Pterophyllum scalare) in São Paulo, Brazil. Nine discarded breeding females with signs of disease were evaluated. The fish exhibited lethargy, loss of appetite, cachexia, skin ulcers, and exophthalmia. At necropsy, four fishes presented macroscopic granulomas in the spleen. Mycobacterium chelonae, M. fortuitum, M. gordonae, M. intracellulare and M. peregrinum were isolated and identified by hsp65 PCR restriction analysis. Histopathological analysis revealed microscopic lesions compatible with mycobacteriosis, and Mycobacterium bacillus were observed by Ziehl-Neelsen stain. Notably, all Mycobacterium species identified in this study have already been reported in human patients; therefore, diseased animals may be a source of infection for people who handle fish and aquariums.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-10T13:56:09Z
2022-10-10T13:56:09Z
2022-06-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1519-6984.262851
Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022.
1519-6984
1678-4375
http://hdl.handle.net/11449/236906
10.1590/1519-6984.262851
S1519-69842024000100325
S1519-69842024000100325.pdf
url http://dx.doi.org/10.1590/1519-6984.262851
http://hdl.handle.net/11449/236906
identifier_str_mv Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2022.
1519-6984
1678-4375
10.1590/1519-6984.262851
S1519-69842024000100325
S1519-69842024000100325.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Instituto Internacional de Ecologia
publisher.none.fl_str_mv Instituto Internacional de Ecologia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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