Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity

Detalhes bibliográficos
Autor(a) principal: Pereira, Iuri Barbosa
Data de Publicação: 2021
Outros Autores: Carvalho, Endrew Henrique de Sousa, Rodrigues, Lais de Brito, Mattos, Bruno Dufau, Magalhaes, Washington Luiz Esteves, Leme, Daniela Morais [UNESP], Krawczyk-Santos, Anna Paula, Taveira, Stephania Fleury, Oliveira, Gisele Augusto Rodrigues de [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/etc.4938
http://hdl.handle.net/11449/209900
Resumo: Thymol, a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we propose use of thymol-loaded biogenic silica nanoparticles (BSiO2#THY NPs); however, the toxicity of this system for aquatic organisms is unknown. Thus, the present study aimed to evaluate the toxicogenetic effects induced by thymol, BSiO2 NP, and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early life stages. We also investigated the impact of BSiO2 aggregation in different exposure media (saline and freshwater). Based on the median lethal concentration at 48 h (LC50(48h)), BSiO2#THY (LC50(48h) = 1.06 mg/L) presented similar toxic potential as thymol (LC50(48h) = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. Because BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of thymol. For zebrafish early life stages, BSiO2#THY (LC50(96h) = 13.13 mg/L) was more toxic than free thymol (LC50(96h) = 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced thymol's availability for this aquatic organism. Also, BSiO2#THY significantly induced sublethal effects as thymol, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems, BSiO2 NP seems to be a safe nanocarrier for agriculture. Environ Toxicol Chem 2021;00:1-9. (c) 2020 SETAC
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spelling Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on EcotoxicityBiocide delivery systemsNanoparticlesAggregationZebrafishArtemia salinaComet assayThymol, a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we propose use of thymol-loaded biogenic silica nanoparticles (BSiO2#THY NPs); however, the toxicity of this system for aquatic organisms is unknown. Thus, the present study aimed to evaluate the toxicogenetic effects induced by thymol, BSiO2 NP, and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early life stages. We also investigated the impact of BSiO2 aggregation in different exposure media (saline and freshwater). Based on the median lethal concentration at 48 h (LC50(48h)), BSiO2#THY (LC50(48h) = 1.06 mg/L) presented similar toxic potential as thymol (LC50(48h) = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. Because BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of thymol. For zebrafish early life stages, BSiO2#THY (LC50(96h) = 13.13 mg/L) was more toxic than free thymol (LC50(96h) = 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced thymol's availability for this aquatic organism. Also, BSiO2#THY significantly induced sublethal effects as thymol, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems, BSiO2 NP seems to be a safe nanocarrier for agriculture. Environ Toxicol Chem 2021;00:1-9. (c) 2020 SETACGoias Research FoundationCoordination of Superior Level Staff ImprovementNational Council for Scientific and Technological Development (INCT/DATREM)Araucaria FoundationUniv Fed Goias, Fac Pharm, Environm Toxicol Res Lab, Goiania, Go, BrazilAalto Univ, Sch Chem Engn, Dept Bioprod & Biosyst, Aalto, FinlandEmbrapa Florestas, Colombo, Parana, BrazilUniv Fed Parana, Dept Genet, Curitiba, Parana, BrazilSao Paulo State Univ, Inst Chem, Natl Inst Alternat Technol Detect Toxicol Evaluat, Araraquara, SP, BrazilUniv Fed Goias, Fac Pharm, Lab Nanosyst & Drug Delivery Syst, Goiania, Go, BrazilSao Paulo State Univ, Inst Chem, Natl Inst Alternat Technol Detect Toxicol Evaluat, Araraquara, SP, BrazilNational Council for Scientific and Technological Development (INCT/DATREM): 465571/2014-0Wiley-BlackwellUniversidade Federal de Goiás (UFG)Aalto UnivEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Univ Fed ParanaUniversidade Estadual Paulista (Unesp)Pereira, Iuri BarbosaCarvalho, Endrew Henrique de SousaRodrigues, Lais de BritoMattos, Bruno DufauMagalhaes, Washington Luiz EstevesLeme, Daniela Morais [UNESP]Krawczyk-Santos, Anna PaulaTaveira, Stephania FleuryOliveira, Gisele Augusto Rodrigues de [UNESP]2021-06-25T12:33:07Z2021-06-25T12:33:07Z2021-01-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article333-341http://dx.doi.org/10.1002/etc.4938Environmental Toxicology And Chemistry. Hoboken: Wiley, v. 40, n. 2, p. 333-341, 2021.0730-7268http://hdl.handle.net/11449/20990010.1002/etc.4938WOS:000607264100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Toxicology And Chemistryinfo:eu-repo/semantics/openAccess2024-06-24T14:51:53Zoai:repositorio.unesp.br:11449/209900Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:59:36.764585Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
title Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
spellingShingle Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
Pereira, Iuri Barbosa
Biocide delivery systems
Nanoparticles
Aggregation
Zebrafish
Artemia salina
Comet assay
title_short Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
title_full Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
title_fullStr Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
title_full_unstemmed Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
title_sort Thymol-Loaded Biogenic Silica Nanoparticles in an Aquatic Environment: The Impact of Particle Aggregation on Ecotoxicity
author Pereira, Iuri Barbosa
author_facet Pereira, Iuri Barbosa
Carvalho, Endrew Henrique de Sousa
Rodrigues, Lais de Brito
Mattos, Bruno Dufau
Magalhaes, Washington Luiz Esteves
Leme, Daniela Morais [UNESP]
Krawczyk-Santos, Anna Paula
Taveira, Stephania Fleury
Oliveira, Gisele Augusto Rodrigues de [UNESP]
author_role author
author2 Carvalho, Endrew Henrique de Sousa
Rodrigues, Lais de Brito
Mattos, Bruno Dufau
Magalhaes, Washington Luiz Esteves
Leme, Daniela Morais [UNESP]
Krawczyk-Santos, Anna Paula
Taveira, Stephania Fleury
Oliveira, Gisele Augusto Rodrigues de [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Goiás (UFG)
Aalto Univ
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Univ Fed Parana
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pereira, Iuri Barbosa
Carvalho, Endrew Henrique de Sousa
Rodrigues, Lais de Brito
Mattos, Bruno Dufau
Magalhaes, Washington Luiz Esteves
Leme, Daniela Morais [UNESP]
Krawczyk-Santos, Anna Paula
Taveira, Stephania Fleury
Oliveira, Gisele Augusto Rodrigues de [UNESP]
dc.subject.por.fl_str_mv Biocide delivery systems
Nanoparticles
Aggregation
Zebrafish
Artemia salina
Comet assay
topic Biocide delivery systems
Nanoparticles
Aggregation
Zebrafish
Artemia salina
Comet assay
description Thymol, a monoterpene phenol, is used as a natural biocide. To circumvent its chemical instability, we propose use of thymol-loaded biogenic silica nanoparticles (BSiO2#THY NPs); however, the toxicity of this system for aquatic organisms is unknown. Thus, the present study aimed to evaluate the toxicogenetic effects induced by thymol, BSiO2 NP, and BSiO2#THY on Artemia salina and zebrafish (Danio rerio) early life stages. We also investigated the impact of BSiO2 aggregation in different exposure media (saline and freshwater). Based on the median lethal concentration at 48 h (LC50(48h)), BSiO2#THY (LC50(48h) = 1.06 mg/L) presented similar toxic potential as thymol (LC50(48h) = 1.03 mg/L) for A. salina, showing that BSiO2 had no influence on BSiO2#THY toxicity. Because BSiO2 aggregated and sedimented faster in A. salina aqueous medium than in the other medium, this NP had lower interaction with this microcrustacean. Thus, BSiO2#THY toxicity for A. salina is probably due to the intrinsic toxicity of thymol. For zebrafish early life stages, BSiO2#THY (LC50(96h) = 13.13 mg/L) was more toxic than free thymol (LC50(96h) = 25.60 mg/L); however, BSiO2 NP has no toxicity for zebrafish early life stages. The lower aggregation of BSiO2 in the freshwater medium compared to the saline medium may have enhanced thymol's availability for this aquatic organism. Also, BSiO2#THY significantly induced sublethal effects as thymol, and both were genotoxic for zebrafish. In conclusion, although BSiO2#THY still needs improvements to ensure its safety for freshwater ecosystems, BSiO2 NP seems to be a safe nanocarrier for agriculture. Environ Toxicol Chem 2021;00:1-9. (c) 2020 SETAC
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T12:33:07Z
2021-06-25T12:33:07Z
2021-01-13
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/etc.4938
Environmental Toxicology And Chemistry. Hoboken: Wiley, v. 40, n. 2, p. 333-341, 2021.
0730-7268
http://hdl.handle.net/11449/209900
10.1002/etc.4938
WOS:000607264100001
url http://dx.doi.org/10.1002/etc.4938
http://hdl.handle.net/11449/209900
identifier_str_mv Environmental Toxicology And Chemistry. Hoboken: Wiley, v. 40, n. 2, p. 333-341, 2021.
0730-7268
10.1002/etc.4938
WOS:000607264100001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Environmental Toxicology And Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 333-341
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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