In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jdent.2022.104246 http://hdl.handle.net/11449/241486 |
Resumo: | Objective: This study assessed the effects of chitosan (CS) on microcosm biofilms derived from saliva of patients with Candida-associated denture stomatitis. Methods: Five removable denture wearers with denture stomatitis were included in the study. The minimum inhibitory concentration (MIC) of CS against clinical isolates of Candida albicans was determined according to the broth microdilution method. Pooled saliva from the donors was used as an inoculum for the formation of biofilms, which were developed during 72 h on acrylic surfaces in the Amsterdam Active Attachment model. The biofilms were then treated with different concentrations of CS, and the antibiofilm effects were evaluated through the quantification of colony-forming units (CFUs), total biomass (TB), metabolic activity (MA), lactic acid production (LAP), and cell viability (by confocal laser scanning microscopy). Chlorhexidine, miconazole, and nystatin were tested as positive controls, while the negative control (NC) was the untreated biofilm. Data were analyzed by 1-way ANOVA and Fischer LSD's post hoc test (α=0.05). Results: MIC values of CS ranged from 500 to 800 µg/mL. For CFUs, 2500 µg/mL CS was the most effective treatment in reducing total anaerobes, mutans streptococci, and Lactobacillus spp., significantly differing from the controls. For C. albicans CFUs, CS and positive controls did not differ from each other but led to significant reductions compared to NC. Regarding TB, MA, LAP, and cell viability, 2500 µg/mL CS promoted the greatest reductions compared to NC. Conclusion: CS has similar or superior effects to conventional active principles on important parameters of oral candidiasis microcosm biofilms. Clinical Relevance: The antibiofilm effects of CS show that this compound has great potential to improve the clinical condition of denture stomatitis patients, and formulations containing this natural polymer could be useful for controlling oral candidiasis. |
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In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasisAntimicrobialsBiofilmsCandidaChitosanOral candidiasisObjective: This study assessed the effects of chitosan (CS) on microcosm biofilms derived from saliva of patients with Candida-associated denture stomatitis. Methods: Five removable denture wearers with denture stomatitis were included in the study. The minimum inhibitory concentration (MIC) of CS against clinical isolates of Candida albicans was determined according to the broth microdilution method. Pooled saliva from the donors was used as an inoculum for the formation of biofilms, which were developed during 72 h on acrylic surfaces in the Amsterdam Active Attachment model. The biofilms were then treated with different concentrations of CS, and the antibiofilm effects were evaluated through the quantification of colony-forming units (CFUs), total biomass (TB), metabolic activity (MA), lactic acid production (LAP), and cell viability (by confocal laser scanning microscopy). Chlorhexidine, miconazole, and nystatin were tested as positive controls, while the negative control (NC) was the untreated biofilm. Data were analyzed by 1-way ANOVA and Fischer LSD's post hoc test (α=0.05). Results: MIC values of CS ranged from 500 to 800 µg/mL. For CFUs, 2500 µg/mL CS was the most effective treatment in reducing total anaerobes, mutans streptococci, and Lactobacillus spp., significantly differing from the controls. For C. albicans CFUs, CS and positive controls did not differ from each other but led to significant reductions compared to NC. Regarding TB, MA, LAP, and cell viability, 2500 µg/mL CS promoted the greatest reductions compared to NC. Conclusion: CS has similar or superior effects to conventional active principles on important parameters of oral candidiasis microcosm biofilms. Clinical Relevance: The antibiofilm effects of CS show that this compound has great potential to improve the clinical condition of denture stomatitis patients, and formulations containing this natural polymer could be useful for controlling oral candidiasis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry São Paulo State University (Unesp)School of Dentistry University of Western São Paulo (UNOESTE)School of Technology and Applied Sciences (FCT) Department of Physics São Paulo State University (Unesp)Postgraduate Program in Health Sciences University of Western São Paulo (UNOESTE), Rua José Bongiovani 700, Presidente PrudenteSchool of Dentistry Araçatuba Department of Preventive and Restorative Dentistry São Paulo State University (Unesp)School of Technology and Applied Sciences (FCT) Department of Physics São Paulo State University (Unesp)FAPESP: 2020/12492–9Universidade Estadual Paulista (UNESP)University of Western São Paulo (UNOESTE)Araujo, Heitor Ceolin [UNESP]Ramírez Carmona, Wilmer [UNESP]Sato, Camilados Santos Oliveira, Marcelo [UNESP]Alves, Guilherme dos Santos GomesMorato, Deborah NunesPessan, Juliano Pelim [UNESP]Monteiro, Douglas Roberto [UNESP]2023-03-01T21:05:30Z2023-03-01T21:05:30Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jdent.2022.104246Journal of Dentistry, v. 125.0300-5712http://hdl.handle.net/11449/24148610.1016/j.jdent.2022.1042462-s2.0-85135690996Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dentistryinfo:eu-repo/semantics/openAccess2024-06-18T18:17:56Zoai:repositorio.unesp.br:11449/241486Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-18T18:17:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis |
title |
In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis |
spellingShingle |
In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis Araujo, Heitor Ceolin [UNESP] Antimicrobials Biofilms Candida Chitosan Oral candidiasis |
title_short |
In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis |
title_full |
In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis |
title_fullStr |
In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis |
title_full_unstemmed |
In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis |
title_sort |
In vitro antimicrobial effects of chitosan on microcosm biofilms of oral candidiasis |
author |
Araujo, Heitor Ceolin [UNESP] |
author_facet |
Araujo, Heitor Ceolin [UNESP] Ramírez Carmona, Wilmer [UNESP] Sato, Camila dos Santos Oliveira, Marcelo [UNESP] Alves, Guilherme dos Santos Gomes Morato, Deborah Nunes Pessan, Juliano Pelim [UNESP] Monteiro, Douglas Roberto [UNESP] |
author_role |
author |
author2 |
Ramírez Carmona, Wilmer [UNESP] Sato, Camila dos Santos Oliveira, Marcelo [UNESP] Alves, Guilherme dos Santos Gomes Morato, Deborah Nunes Pessan, Juliano Pelim [UNESP] Monteiro, Douglas Roberto [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Western São Paulo (UNOESTE) |
dc.contributor.author.fl_str_mv |
Araujo, Heitor Ceolin [UNESP] Ramírez Carmona, Wilmer [UNESP] Sato, Camila dos Santos Oliveira, Marcelo [UNESP] Alves, Guilherme dos Santos Gomes Morato, Deborah Nunes Pessan, Juliano Pelim [UNESP] Monteiro, Douglas Roberto [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobials Biofilms Candida Chitosan Oral candidiasis |
topic |
Antimicrobials Biofilms Candida Chitosan Oral candidiasis |
description |
Objective: This study assessed the effects of chitosan (CS) on microcosm biofilms derived from saliva of patients with Candida-associated denture stomatitis. Methods: Five removable denture wearers with denture stomatitis were included in the study. The minimum inhibitory concentration (MIC) of CS against clinical isolates of Candida albicans was determined according to the broth microdilution method. Pooled saliva from the donors was used as an inoculum for the formation of biofilms, which were developed during 72 h on acrylic surfaces in the Amsterdam Active Attachment model. The biofilms were then treated with different concentrations of CS, and the antibiofilm effects were evaluated through the quantification of colony-forming units (CFUs), total biomass (TB), metabolic activity (MA), lactic acid production (LAP), and cell viability (by confocal laser scanning microscopy). Chlorhexidine, miconazole, and nystatin were tested as positive controls, while the negative control (NC) was the untreated biofilm. Data were analyzed by 1-way ANOVA and Fischer LSD's post hoc test (α=0.05). Results: MIC values of CS ranged from 500 to 800 µg/mL. For CFUs, 2500 µg/mL CS was the most effective treatment in reducing total anaerobes, mutans streptococci, and Lactobacillus spp., significantly differing from the controls. For C. albicans CFUs, CS and positive controls did not differ from each other but led to significant reductions compared to NC. Regarding TB, MA, LAP, and cell viability, 2500 µg/mL CS promoted the greatest reductions compared to NC. Conclusion: CS has similar or superior effects to conventional active principles on important parameters of oral candidiasis microcosm biofilms. Clinical Relevance: The antibiofilm effects of CS show that this compound has great potential to improve the clinical condition of denture stomatitis patients, and formulations containing this natural polymer could be useful for controlling oral candidiasis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-01 2023-03-01T21:05:30Z 2023-03-01T21:05:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jdent.2022.104246 Journal of Dentistry, v. 125. 0300-5712 http://hdl.handle.net/11449/241486 10.1016/j.jdent.2022.104246 2-s2.0-85135690996 |
url |
http://dx.doi.org/10.1016/j.jdent.2022.104246 http://hdl.handle.net/11449/241486 |
identifier_str_mv |
Journal of Dentistry, v. 125. 0300-5712 10.1016/j.jdent.2022.104246 2-s2.0-85135690996 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045295959834624 |