Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612015073 http://hdl.handle.net/11449/177668 |
Resumo: | One of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serological and parasitological examinations and PCR. Positivity by PCR was higher than by serology and by parasitological examinations and showed a tendency to decrease over time, while serology tended to increase after six months. Concomitant positivity in all tests was observed in 10.4% of the samples, and negativity in 29.1%. Overall sensitivity ranged from 43.6 to 64.1%, and was not uniform over time. The proportion of dogs with or without clinical signs was not different by cytology or PCR but PCR was able to identify a larger number of asymptomatic dogs compared to ELISA and immunochromatography. PCR can be useful for surveillance of areas where cases of canine VL have not yet been detected and in which control strategies can be implemented to limit the spread of the disease. Despite the advance in diagnostic tools CVL diagnosis remains a challenge. |
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Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinalSerological, parasitological and molecular tests for canine visceral leishmaniosis diagnosis in a longitudinal studyCohort studiesDiagnosisLeishmania infantum chagasiParasitological evaluationPCRSerologyOne of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serological and parasitological examinations and PCR. Positivity by PCR was higher than by serology and by parasitological examinations and showed a tendency to decrease over time, while serology tended to increase after six months. Concomitant positivity in all tests was observed in 10.4% of the samples, and negativity in 29.1%. Overall sensitivity ranged from 43.6 to 64.1%, and was not uniform over time. The proportion of dogs with or without clinical signs was not different by cytology or PCR but PCR was able to identify a larger number of asymptomatic dogs compared to ELISA and immunochromatography. PCR can be useful for surveillance of areas where cases of canine VL have not yet been detected and in which control strategies can be implemented to limit the spread of the disease. Despite the advance in diagnostic tools CVL diagnosis remains a challenge.Universidade Estadual Paulista – UNESPAutonomous VeterinarianCompanhia Integrada de Desenvolvimento Agrícola de Santa CatarinaInstituto de Ciências Biomédicas Universidade de São Paulo – USPInstituto Adolfo Lutz – IALUniversidade de São Paulo – USPUniversidade Estadual Paulista – UNESPUniversidade Estadual Paulista (Unesp)Autonomous VeterinarianCompanhia Integrada de Desenvolvimento Agrícola de Santa CatarinaUniversidade de São Paulo (USP)Instituto Adolfo Lutz – IALNunes, Cáris Maroni [UNESP]de Lima, Valéria Marçal Félix [UNESP]de Melo, Guilherme Dias [UNESP]de Paula, Henrique BorgesPereira, Maria Esther GonçalvesTronco, Cristianade Melo TrinconiHiramoto, Roberto MitsuyoshiLaurenti, Marcia DalastraBurattini, Marcelo Nascimento2018-12-11T17:26:33Z2018-12-11T17:26:33Z2015-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article402-409application/pdfhttp://dx.doi.org/10.1590/S1984-29612015073Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 4, p. 402-409, 2015.0103-846Xhttp://hdl.handle.net/11449/17766810.1590/S1984-29612015073S1984-296120150004004022-s2.0-84951291377S1984-29612015000400402.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-09-04T19:16:07Zoai:repositorio.unesp.br:11449/177668Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal Serological, parasitological and molecular tests for canine visceral leishmaniosis diagnosis in a longitudinal study |
title |
Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal |
spellingShingle |
Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal Nunes, Cáris Maroni [UNESP] Cohort studies Diagnosis Leishmania infantum chagasi Parasitological evaluation PCR Serology |
title_short |
Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal |
title_full |
Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal |
title_fullStr |
Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal |
title_full_unstemmed |
Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal |
title_sort |
Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal |
author |
Nunes, Cáris Maroni [UNESP] |
author_facet |
Nunes, Cáris Maroni [UNESP] de Lima, Valéria Marçal Félix [UNESP] de Melo, Guilherme Dias [UNESP] de Paula, Henrique Borges Pereira, Maria Esther Gonçalves Tronco, Cristianade Melo Trinconi Hiramoto, Roberto Mitsuyoshi Laurenti, Marcia Dalastra Burattini, Marcelo Nascimento |
author_role |
author |
author2 |
de Lima, Valéria Marçal Félix [UNESP] de Melo, Guilherme Dias [UNESP] de Paula, Henrique Borges Pereira, Maria Esther Gonçalves Tronco, Cristianade Melo Trinconi Hiramoto, Roberto Mitsuyoshi Laurenti, Marcia Dalastra Burattini, Marcelo Nascimento |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Autonomous Veterinarian Companhia Integrada de Desenvolvimento Agrícola de Santa Catarina Universidade de São Paulo (USP) Instituto Adolfo Lutz – IAL |
dc.contributor.author.fl_str_mv |
Nunes, Cáris Maroni [UNESP] de Lima, Valéria Marçal Félix [UNESP] de Melo, Guilherme Dias [UNESP] de Paula, Henrique Borges Pereira, Maria Esther Gonçalves Tronco, Cristianade Melo Trinconi Hiramoto, Roberto Mitsuyoshi Laurenti, Marcia Dalastra Burattini, Marcelo Nascimento |
dc.subject.por.fl_str_mv |
Cohort studies Diagnosis Leishmania infantum chagasi Parasitological evaluation PCR Serology |
topic |
Cohort studies Diagnosis Leishmania infantum chagasi Parasitological evaluation PCR Serology |
description |
One of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serological and parasitological examinations and PCR. Positivity by PCR was higher than by serology and by parasitological examinations and showed a tendency to decrease over time, while serology tended to increase after six months. Concomitant positivity in all tests was observed in 10.4% of the samples, and negativity in 29.1%. Overall sensitivity ranged from 43.6 to 64.1%, and was not uniform over time. The proportion of dogs with or without clinical signs was not different by cytology or PCR but PCR was able to identify a larger number of asymptomatic dogs compared to ELISA and immunochromatography. PCR can be useful for surveillance of areas where cases of canine VL have not yet been detected and in which control strategies can be implemented to limit the spread of the disease. Despite the advance in diagnostic tools CVL diagnosis remains a challenge. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 2018-12-11T17:26:33Z 2018-12-11T17:26:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612015073 Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 4, p. 402-409, 2015. 0103-846X http://hdl.handle.net/11449/177668 10.1590/S1984-29612015073 S1984-29612015000400402 2-s2.0-84951291377 S1984-29612015000400402.pdf |
url |
http://dx.doi.org/10.1590/S1984-29612015073 http://hdl.handle.net/11449/177668 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 4, p. 402-409, 2015. 0103-846X 10.1590/S1984-29612015073 S1984-29612015000400402 2-s2.0-84951291377 S1984-29612015000400402.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
402-409 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021418647158784 |