Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal

Detalhes bibliográficos
Autor(a) principal: Nunes, Cáris Maroni [UNESP]
Data de Publicação: 2015
Outros Autores: de Lima, Valéria Marçal Félix [UNESP], de Melo, Guilherme Dias [UNESP], de Paula, Henrique Borges, Pereira, Maria Esther Gonçalves, Tronco, Cristianade Melo Trinconi, Hiramoto, Roberto Mitsuyoshi, Laurenti, Marcia Dalastra, Burattini, Marcelo Nascimento
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1984-29612015073
http://hdl.handle.net/11449/177668
Resumo: One of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serological and parasitological examinations and PCR. Positivity by PCR was higher than by serology and by parasitological examinations and showed a tendency to decrease over time, while serology tended to increase after six months. Concomitant positivity in all tests was observed in 10.4% of the samples, and negativity in 29.1%. Overall sensitivity ranged from 43.6 to 64.1%, and was not uniform over time. The proportion of dogs with or without clinical signs was not different by cytology or PCR but PCR was able to identify a larger number of asymptomatic dogs compared to ELISA and immunochromatography. PCR can be useful for surveillance of areas where cases of canine VL have not yet been detected and in which control strategies can be implemented to limit the spread of the disease. Despite the advance in diagnostic tools CVL diagnosis remains a challenge.
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spelling Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinalSerological, parasitological and molecular tests for canine visceral leishmaniosis diagnosis in a longitudinal studyCohort studiesDiagnosisLeishmania infantum chagasiParasitological evaluationPCRSerologyOne of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serological and parasitological examinations and PCR. Positivity by PCR was higher than by serology and by parasitological examinations and showed a tendency to decrease over time, while serology tended to increase after six months. Concomitant positivity in all tests was observed in 10.4% of the samples, and negativity in 29.1%. Overall sensitivity ranged from 43.6 to 64.1%, and was not uniform over time. The proportion of dogs with or without clinical signs was not different by cytology or PCR but PCR was able to identify a larger number of asymptomatic dogs compared to ELISA and immunochromatography. PCR can be useful for surveillance of areas where cases of canine VL have not yet been detected and in which control strategies can be implemented to limit the spread of the disease. Despite the advance in diagnostic tools CVL diagnosis remains a challenge.Universidade Estadual Paulista – UNESPAutonomous VeterinarianCompanhia Integrada de Desenvolvimento Agrícola de Santa CatarinaInstituto de Ciências Biomédicas Universidade de São Paulo – USPInstituto Adolfo Lutz – IALUniversidade de São Paulo – USPUniversidade Estadual Paulista – UNESPUniversidade Estadual Paulista (Unesp)Autonomous VeterinarianCompanhia Integrada de Desenvolvimento Agrícola de Santa CatarinaUniversidade de São Paulo (USP)Instituto Adolfo Lutz – IALNunes, Cáris Maroni [UNESP]de Lima, Valéria Marçal Félix [UNESP]de Melo, Guilherme Dias [UNESP]de Paula, Henrique BorgesPereira, Maria Esther GonçalvesTronco, Cristianade Melo TrinconiHiramoto, Roberto MitsuyoshiLaurenti, Marcia DalastraBurattini, Marcelo Nascimento2018-12-11T17:26:33Z2018-12-11T17:26:33Z2015-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article402-409application/pdfhttp://dx.doi.org/10.1590/S1984-29612015073Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 4, p. 402-409, 2015.0103-846Xhttp://hdl.handle.net/11449/17766810.1590/S1984-29612015073S1984-296120150004004022-s2.0-84951291377S1984-29612015000400402.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-09-04T19:16:07Zoai:repositorio.unesp.br:11449/177668Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
Serological, parasitological and molecular tests for canine visceral leishmaniosis diagnosis in a longitudinal study
title Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
spellingShingle Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
Nunes, Cáris Maroni [UNESP]
Cohort studies
Diagnosis
Leishmania infantum chagasi
Parasitological evaluation
PCR
Serology
title_short Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
title_full Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
title_fullStr Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
title_full_unstemmed Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
title_sort Testes sorológicos, parasitológicos e moleculares para o diagnóstico da leishmaniose visceral canina em estudo longitudinal
author Nunes, Cáris Maroni [UNESP]
author_facet Nunes, Cáris Maroni [UNESP]
de Lima, Valéria Marçal Félix [UNESP]
de Melo, Guilherme Dias [UNESP]
de Paula, Henrique Borges
Pereira, Maria Esther Gonçalves
Tronco, Cristianade Melo Trinconi
Hiramoto, Roberto Mitsuyoshi
Laurenti, Marcia Dalastra
Burattini, Marcelo Nascimento
author_role author
author2 de Lima, Valéria Marçal Félix [UNESP]
de Melo, Guilherme Dias [UNESP]
de Paula, Henrique Borges
Pereira, Maria Esther Gonçalves
Tronco, Cristianade Melo Trinconi
Hiramoto, Roberto Mitsuyoshi
Laurenti, Marcia Dalastra
Burattini, Marcelo Nascimento
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Autonomous Veterinarian
Companhia Integrada de Desenvolvimento Agrícola de Santa Catarina
Universidade de São Paulo (USP)
Instituto Adolfo Lutz – IAL
dc.contributor.author.fl_str_mv Nunes, Cáris Maroni [UNESP]
de Lima, Valéria Marçal Félix [UNESP]
de Melo, Guilherme Dias [UNESP]
de Paula, Henrique Borges
Pereira, Maria Esther Gonçalves
Tronco, Cristianade Melo Trinconi
Hiramoto, Roberto Mitsuyoshi
Laurenti, Marcia Dalastra
Burattini, Marcelo Nascimento
dc.subject.por.fl_str_mv Cohort studies
Diagnosis
Leishmania infantum chagasi
Parasitological evaluation
PCR
Serology
topic Cohort studies
Diagnosis
Leishmania infantum chagasi
Parasitological evaluation
PCR
Serology
description One of the measures to control visceral leishmaniosis (VL) in Brazil is the identification and culling of the canine reservoir. There is much controversy concerning this strategy, including the proper identification of positive dogs and the fact that the host-parasite relationship changes over time make it more challenging. A dynamic cohort of 62 dogs was followed every three months using serological and parasitological examinations and PCR. Positivity by PCR was higher than by serology and by parasitological examinations and showed a tendency to decrease over time, while serology tended to increase after six months. Concomitant positivity in all tests was observed in 10.4% of the samples, and negativity in 29.1%. Overall sensitivity ranged from 43.6 to 64.1%, and was not uniform over time. The proportion of dogs with or without clinical signs was not different by cytology or PCR but PCR was able to identify a larger number of asymptomatic dogs compared to ELISA and immunochromatography. PCR can be useful for surveillance of areas where cases of canine VL have not yet been detected and in which control strategies can be implemented to limit the spread of the disease. Despite the advance in diagnostic tools CVL diagnosis remains a challenge.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-01
2018-12-11T17:26:33Z
2018-12-11T17:26:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1984-29612015073
Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 4, p. 402-409, 2015.
0103-846X
http://hdl.handle.net/11449/177668
10.1590/S1984-29612015073
S1984-29612015000400402
2-s2.0-84951291377
S1984-29612015000400402.pdf
url http://dx.doi.org/10.1590/S1984-29612015073
http://hdl.handle.net/11449/177668
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 4, p. 402-409, 2015.
0103-846X
10.1590/S1984-29612015073
S1984-29612015000400402
2-s2.0-84951291377
S1984-29612015000400402.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 402-409
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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