Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0158400 http://hdl.handle.net/11449/173170 |
Resumo: | The present study evaluated the effectiveness of a dicalcium and tetracalcium phosphatebased desensitizer in reducing dentin permeability in vitro. Dentin fluid flow was measured before and after treatment of dentin with patent dentinal tubules using 1 or 3 applications of the dicalcium and tetracalcium phosphate containing agent TeethmateTM (TM) and comparing the results with two sodium fluoride varnishes VellaTM (VLA) and VanishTM (VAN), after storage in artificial saliva for 24 h, 48 h and 7 days. Significant differences were observed among the 4 methods employed for reducing dentin permeability (p < 0.001) and the 3 posttreatment times (p < 0.001). VLA and VAN never achieved 50% permeability reductions consistently in any of the 3 time periods. Only the calcium phosphate-based desensitizer applied for 3 times consistently reduced dentin permeability by 50% after 24 h. When applied once, the permeability reduction of TM increased progressively over the 3 time periods. After 7 days, only one and three applications of the calcium phosphate-based desensitizer consistently reduced dentin permeability by more than 50%. Permeability reductions corresponded well with scanning electron microscopy examination of dentinal tubule orifice occlusion in dentin specimens treated with the agents. Overall, the dicalcium and tetracalcium phosphate-based desensitizer is effective in reducing dentin permeability via a tubule occlusion mechanism. The ability of the agent to reduce dentin permeability renders it to be potentially useful as a clinical dentin desensitizing agent, which has to be confirmed in future clinical studies. By contrast, the two sodium fluoride varnishes are not effective in dentin permeability reduction and should be considered as topical fluoride delivering agents rather than tubular orifice-blocking agents. |
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Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeabilityThe present study evaluated the effectiveness of a dicalcium and tetracalcium phosphatebased desensitizer in reducing dentin permeability in vitro. Dentin fluid flow was measured before and after treatment of dentin with patent dentinal tubules using 1 or 3 applications of the dicalcium and tetracalcium phosphate containing agent TeethmateTM (TM) and comparing the results with two sodium fluoride varnishes VellaTM (VLA) and VanishTM (VAN), after storage in artificial saliva for 24 h, 48 h and 7 days. Significant differences were observed among the 4 methods employed for reducing dentin permeability (p < 0.001) and the 3 posttreatment times (p < 0.001). VLA and VAN never achieved 50% permeability reductions consistently in any of the 3 time periods. Only the calcium phosphate-based desensitizer applied for 3 times consistently reduced dentin permeability by 50% after 24 h. When applied once, the permeability reduction of TM increased progressively over the 3 time periods. After 7 days, only one and three applications of the calcium phosphate-based desensitizer consistently reduced dentin permeability by more than 50%. Permeability reductions corresponded well with scanning electron microscopy examination of dentinal tubule orifice occlusion in dentin specimens treated with the agents. Overall, the dicalcium and tetracalcium phosphate-based desensitizer is effective in reducing dentin permeability via a tubule occlusion mechanism. The ability of the agent to reduce dentin permeability renders it to be potentially useful as a clinical dentin desensitizing agent, which has to be confirmed in future clinical studies. By contrast, the two sodium fluoride varnishes are not effective in dentin permeability reduction and should be considered as topical fluoride delivering agents rather than tubular orifice-blocking agents.National Institute of Dental and Craniofacial ResearchDepartment of Prosthodontics Peking University School and Hospital of StomatologyDepartment of Cariology and Operative Dentistry Graduate School of Medical and Dental Sciences Tokyo Medical and Dental UniversityDepartment of Orthodontics and Pediatric Dentistry Universidade Estadual Paulista-UNESP Araraquara Dental SchoolDental College of Georgia Augusta UniversityState Key Laboratory of Military Stomatology National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Oral Diseases Department of Prosthodontics School of Stomatology Fourth Military Medical UniversityDepartment of Orthodontics and Pediatric Dentistry Universidade Estadual Paulista-UNESP Araraquara Dental SchoolNational Institute of Dental and Craniofacial Research: R01 DE015306.School and Hospital of StomatologyTokyo Medical and Dental UniversityUniversidade Estadual Paulista (Unesp)Augusta UniversityFourth Military Medical UniversityZhou, JianfengChiba, AyakaScheffel, Debora L. S. [UNESP]Hebling, Josimeri [UNESP]Agee, KelliNiu, Li-NaTay, Franklin R.Pashley, David H.2018-12-11T17:03:58Z2018-12-11T17:03:58Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0158400PLoS ONE, v. 11, n. 6, 2016.1932-6203http://hdl.handle.net/11449/17317010.1371/journal.pone.01584002-s2.0-849772703602-s2.0-84977270360.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2023-12-15T06:15:22Zoai:repositorio.unesp.br:11449/173170Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:22:38.049501Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability |
title |
Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability |
spellingShingle |
Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability Zhou, Jianfeng |
title_short |
Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability |
title_full |
Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability |
title_fullStr |
Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability |
title_full_unstemmed |
Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability |
title_sort |
Effects of a dicalcium and tetracalcium phosphate-based desensitizer on in vitro dentin permeability |
author |
Zhou, Jianfeng |
author_facet |
Zhou, Jianfeng Chiba, Ayaka Scheffel, Debora L. S. [UNESP] Hebling, Josimeri [UNESP] Agee, Kelli Niu, Li-Na Tay, Franklin R. Pashley, David H. |
author_role |
author |
author2 |
Chiba, Ayaka Scheffel, Debora L. S. [UNESP] Hebling, Josimeri [UNESP] Agee, Kelli Niu, Li-Na Tay, Franklin R. Pashley, David H. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
School and Hospital of Stomatology Tokyo Medical and Dental University Universidade Estadual Paulista (Unesp) Augusta University Fourth Military Medical University |
dc.contributor.author.fl_str_mv |
Zhou, Jianfeng Chiba, Ayaka Scheffel, Debora L. S. [UNESP] Hebling, Josimeri [UNESP] Agee, Kelli Niu, Li-Na Tay, Franklin R. Pashley, David H. |
description |
The present study evaluated the effectiveness of a dicalcium and tetracalcium phosphatebased desensitizer in reducing dentin permeability in vitro. Dentin fluid flow was measured before and after treatment of dentin with patent dentinal tubules using 1 or 3 applications of the dicalcium and tetracalcium phosphate containing agent TeethmateTM (TM) and comparing the results with two sodium fluoride varnishes VellaTM (VLA) and VanishTM (VAN), after storage in artificial saliva for 24 h, 48 h and 7 days. Significant differences were observed among the 4 methods employed for reducing dentin permeability (p < 0.001) and the 3 posttreatment times (p < 0.001). VLA and VAN never achieved 50% permeability reductions consistently in any of the 3 time periods. Only the calcium phosphate-based desensitizer applied for 3 times consistently reduced dentin permeability by 50% after 24 h. When applied once, the permeability reduction of TM increased progressively over the 3 time periods. After 7 days, only one and three applications of the calcium phosphate-based desensitizer consistently reduced dentin permeability by more than 50%. Permeability reductions corresponded well with scanning electron microscopy examination of dentinal tubule orifice occlusion in dentin specimens treated with the agents. Overall, the dicalcium and tetracalcium phosphate-based desensitizer is effective in reducing dentin permeability via a tubule occlusion mechanism. The ability of the agent to reduce dentin permeability renders it to be potentially useful as a clinical dentin desensitizing agent, which has to be confirmed in future clinical studies. By contrast, the two sodium fluoride varnishes are not effective in dentin permeability reduction and should be considered as topical fluoride delivering agents rather than tubular orifice-blocking agents. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 2018-12-11T17:03:58Z 2018-12-11T17:03:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0158400 PLoS ONE, v. 11, n. 6, 2016. 1932-6203 http://hdl.handle.net/11449/173170 10.1371/journal.pone.0158400 2-s2.0-84977270360 2-s2.0-84977270360.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0158400 http://hdl.handle.net/11449/173170 |
identifier_str_mv |
PLoS ONE, v. 11, n. 6, 2016. 1932-6203 10.1371/journal.pone.0158400 2-s2.0-84977270360 2-s2.0-84977270360.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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