Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.scitotenv.2018.07.438 http://hdl.handle.net/11449/186875 |
Resumo: | The objective of this study was to verify the presence of antimicrobial resistant strains of Escherichia coli in pig farms and to use it as a biomarker to evaluate phenotypic and genotypic profiles of antimicrobial susceptibility, as well as the presence of Extended Spectrum Beta-lactamases (ESBLs) and fluoroquinolone resistance genes. Several samples (n = 306) collected from swine farms (n = 100) of Southern Brazil were used for E. coli isolation: 103 of swine feces, 105 of water, and 98 of soil. E. coli isolates were submitted to the disk-diffusion test to verify their antimicrobial susceptibility, to disk-approximation test to detect ESBL-producers, and to PCR analysis to search for ESBLs genes (blaCTY-M2, blaSHV-1, blaTEM-1, blaCTX-M2, blaOXA-1, blaPSE-1) and quinolone resistance genes (qnrA, qnrB and qnrS). The percentage of E. coli isolates found in feces, water and soil samples was 66.02%, 30.48% and 35.71%, respectively. The highest percentages of resistance were obtained for sulfamethoxazole associated with trimethoprim (63.70%), colistin (45.19%) and enrofloxacin (39.26%). Regarding the levels of multidrug resistance, 37.04% of the isolates were resistant to three or more classes of antimicrobials. The most common profile (16%) of multirresistance was GEM-SUT-ENO-COL. The index of multiple resistance to antimicrobials (IRMA) was above 0.2 in 78% of the multiresistant isolates. Out of 135 E. coli isolates, 7.41% was ESBL-producers, of which 50% showed the blaCMY-M2 gene, 40% the blaTEM-1 and 70% the qnrS gene. Of non-ESBL-producing strains resistant to enrofloxacin, 13.04% were positives for qnrS gene. These results demonstrated the presence of fecal contamination in the environment, in addition to high resistance indexes for several antimicrobials, including beta-lactams and fluoroquinolones, which was confirmed by the genetic detection of ESBLs and qnr genes. |
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Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern BrazilESBLsEscherichia coliFecal contaminationFluoroquinolonesResistanceThe objective of this study was to verify the presence of antimicrobial resistant strains of Escherichia coli in pig farms and to use it as a biomarker to evaluate phenotypic and genotypic profiles of antimicrobial susceptibility, as well as the presence of Extended Spectrum Beta-lactamases (ESBLs) and fluoroquinolone resistance genes. Several samples (n = 306) collected from swine farms (n = 100) of Southern Brazil were used for E. coli isolation: 103 of swine feces, 105 of water, and 98 of soil. E. coli isolates were submitted to the disk-diffusion test to verify their antimicrobial susceptibility, to disk-approximation test to detect ESBL-producers, and to PCR analysis to search for ESBLs genes (blaCTY-M2, blaSHV-1, blaTEM-1, blaCTX-M2, blaOXA-1, blaPSE-1) and quinolone resistance genes (qnrA, qnrB and qnrS). The percentage of E. coli isolates found in feces, water and soil samples was 66.02%, 30.48% and 35.71%, respectively. The highest percentages of resistance were obtained for sulfamethoxazole associated with trimethoprim (63.70%), colistin (45.19%) and enrofloxacin (39.26%). Regarding the levels of multidrug resistance, 37.04% of the isolates were resistant to three or more classes of antimicrobials. The most common profile (16%) of multirresistance was GEM-SUT-ENO-COL. The index of multiple resistance to antimicrobials (IRMA) was above 0.2 in 78% of the multiresistant isolates. Out of 135 E. coli isolates, 7.41% was ESBL-producers, of which 50% showed the blaCMY-M2 gene, 40% the blaTEM-1 and 70% the qnrS gene. Of non-ESBL-producing strains resistant to enrofloxacin, 13.04% were positives for qnrS gene. These results demonstrated the presence of fecal contamination in the environment, in addition to high resistance indexes for several antimicrobials, including beta-lactams and fluoroquinolones, which was confirmed by the genetic detection of ESBLs and qnr genes.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Post Graduation Program in Animal Science State University of Santa Catarina (UDESC) Western Center of EducationGraduate in Animal Science State University of Santa Catarina (UDESC) Western Center of EducationDepartment of Animal Science State University of Santa Catarina (UDESC) Western Center of EducationDepartment of Mathematics Paulista State University Júlio de Mesquita Filho (UNESP)Department of Mathematics Paulista State University Júlio de Mesquita Filho (UNESP)Western Center of EducationUniversidade Estadual Paulista (Unesp)Brisola, Maiara CristianeCrecencio, Regiane BoarettoBitner, Dinael SimãoFrigo, AngélicaRampazzo, LuanaStefani, Lenita MouraFaria, Gláucia Amorim [UNESP]2019-10-06T15:18:27Z2019-10-06T15:18:27Z2019-01-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article362-368http://dx.doi.org/10.1016/j.scitotenv.2018.07.438Science of the Total Environment, v. 647, p. 362-368.1879-10260048-9697http://hdl.handle.net/11449/18687510.1016/j.scitotenv.2018.07.4382-s2.0-85050865067Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScience of the Total Environmentinfo:eu-repo/semantics/openAccess2021-10-23T20:19:26Zoai:repositorio.unesp.br:11449/186875Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:23:38.438914Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil |
title |
Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil |
spellingShingle |
Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil Brisola, Maiara Cristiane ESBLs Escherichia coli Fecal contamination Fluoroquinolones Resistance |
title_short |
Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil |
title_full |
Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil |
title_fullStr |
Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil |
title_full_unstemmed |
Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil |
title_sort |
Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil |
author |
Brisola, Maiara Cristiane |
author_facet |
Brisola, Maiara Cristiane Crecencio, Regiane Boaretto Bitner, Dinael Simão Frigo, Angélica Rampazzo, Luana Stefani, Lenita Moura Faria, Gláucia Amorim [UNESP] |
author_role |
author |
author2 |
Crecencio, Regiane Boaretto Bitner, Dinael Simão Frigo, Angélica Rampazzo, Luana Stefani, Lenita Moura Faria, Gláucia Amorim [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Western Center of Education Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Brisola, Maiara Cristiane Crecencio, Regiane Boaretto Bitner, Dinael Simão Frigo, Angélica Rampazzo, Luana Stefani, Lenita Moura Faria, Gláucia Amorim [UNESP] |
dc.subject.por.fl_str_mv |
ESBLs Escherichia coli Fecal contamination Fluoroquinolones Resistance |
topic |
ESBLs Escherichia coli Fecal contamination Fluoroquinolones Resistance |
description |
The objective of this study was to verify the presence of antimicrobial resistant strains of Escherichia coli in pig farms and to use it as a biomarker to evaluate phenotypic and genotypic profiles of antimicrobial susceptibility, as well as the presence of Extended Spectrum Beta-lactamases (ESBLs) and fluoroquinolone resistance genes. Several samples (n = 306) collected from swine farms (n = 100) of Southern Brazil were used for E. coli isolation: 103 of swine feces, 105 of water, and 98 of soil. E. coli isolates were submitted to the disk-diffusion test to verify their antimicrobial susceptibility, to disk-approximation test to detect ESBL-producers, and to PCR analysis to search for ESBLs genes (blaCTY-M2, blaSHV-1, blaTEM-1, blaCTX-M2, blaOXA-1, blaPSE-1) and quinolone resistance genes (qnrA, qnrB and qnrS). The percentage of E. coli isolates found in feces, water and soil samples was 66.02%, 30.48% and 35.71%, respectively. The highest percentages of resistance were obtained for sulfamethoxazole associated with trimethoprim (63.70%), colistin (45.19%) and enrofloxacin (39.26%). Regarding the levels of multidrug resistance, 37.04% of the isolates were resistant to three or more classes of antimicrobials. The most common profile (16%) of multirresistance was GEM-SUT-ENO-COL. The index of multiple resistance to antimicrobials (IRMA) was above 0.2 in 78% of the multiresistant isolates. Out of 135 E. coli isolates, 7.41% was ESBL-producers, of which 50% showed the blaCMY-M2 gene, 40% the blaTEM-1 and 70% the qnrS gene. Of non-ESBL-producing strains resistant to enrofloxacin, 13.04% were positives for qnrS gene. These results demonstrated the presence of fecal contamination in the environment, in addition to high resistance indexes for several antimicrobials, including beta-lactams and fluoroquinolones, which was confirmed by the genetic detection of ESBLs and qnr genes. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:18:27Z 2019-10-06T15:18:27Z 2019-01-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.scitotenv.2018.07.438 Science of the Total Environment, v. 647, p. 362-368. 1879-1026 0048-9697 http://hdl.handle.net/11449/186875 10.1016/j.scitotenv.2018.07.438 2-s2.0-85050865067 |
url |
http://dx.doi.org/10.1016/j.scitotenv.2018.07.438 http://hdl.handle.net/11449/186875 |
identifier_str_mv |
Science of the Total Environment, v. 647, p. 362-368. 1879-1026 0048-9697 10.1016/j.scitotenv.2018.07.438 2-s2.0-85050865067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Science of the Total Environment |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
362-368 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129196458770432 |