Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis

Detalhes bibliográficos
Autor(a) principal: Castro, Camila Ferreira Bannwart [UNESP]
Data de Publicação: 2009
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/100582
Resumo: Silibinin is the major active component of silymarin (Silybum marianum), a polyphenolic plant flavonoid that has anti-inflammatory, cytoprotective and anticarcinogenic effects. The modulatory effect of silibinin on monocyte function against Paracoccidioides brasiliensis (Pb18) has not yet been demonstrated. The present study investigated whether the effect of silibinin on NF-kB pathways may affects the production of tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), Transforming growth factor beta (TGF-β1), prostaglandin E2 (PGE2) and nitric oxide (NO), as well as the fungicidal activity of human monocytes challenged in vitro with Pb18. Peripheral blood monocytes from healthy individuals were treated with or without 5 uM and 50 uM silibinin and challenged with Pb18 or were stimulated with lipopolissaccharide (LPS). After 18 h of culture TNF-α, IL-10, TGF-β1 and PGE2 were determined by ELISA, while NO release was quantified by the accumulation of nitrite in the supernatants using the standard Griess assay. Fungicidal activity of monocytes against Pb18 was assessed after cell incubation with interferon-gamma, culture with or without silibinin for 18 h and challenge with Pb18 for 4h. NF-kB activation in the cultures was evaluated by flow cytometry and ELISA after monocyte stimulation with Pb18 or LPS for 30 min. Silibinin at 50uM concentration partially inhibited p65NF-kB activation as observed by reduction in the number of cells expressing this factor, that was confirmed by low concentration detection of p65NF-kB in the nucleus. This silibinin effects resulted in suppression of TNF-α, IL-10, TGF-β1 and PGE2 and NO production, but did not affect fungicidal activity of monocytes against Pb18. The modulatory effect of silibinin on the monocytes inflammatory response against P. brasiliensis might be important to establish an efficient and beneficial immune response of the host against the fungus
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spelling Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensisImunologiaAgentes anti-inflamatoriosParacoccidiodomicoseImmunologySilibinin is the major active component of silymarin (Silybum marianum), a polyphenolic plant flavonoid that has anti-inflammatory, cytoprotective and anticarcinogenic effects. The modulatory effect of silibinin on monocyte function against Paracoccidioides brasiliensis (Pb18) has not yet been demonstrated. The present study investigated whether the effect of silibinin on NF-kB pathways may affects the production of tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), Transforming growth factor beta (TGF-β1), prostaglandin E2 (PGE2) and nitric oxide (NO), as well as the fungicidal activity of human monocytes challenged in vitro with Pb18. Peripheral blood monocytes from healthy individuals were treated with or without 5 uM and 50 uM silibinin and challenged with Pb18 or were stimulated with lipopolissaccharide (LPS). After 18 h of culture TNF-α, IL-10, TGF-β1 and PGE2 were determined by ELISA, while NO release was quantified by the accumulation of nitrite in the supernatants using the standard Griess assay. Fungicidal activity of monocytes against Pb18 was assessed after cell incubation with interferon-gamma, culture with or without silibinin for 18 h and challenge with Pb18 for 4h. NF-kB activation in the cultures was evaluated by flow cytometry and ELISA after monocyte stimulation with Pb18 or LPS for 30 min. Silibinin at 50uM concentration partially inhibited p65NF-kB activation as observed by reduction in the number of cells expressing this factor, that was confirmed by low concentration detection of p65NF-kB in the nucleus. This silibinin effects resulted in suppression of TNF-α, IL-10, TGF-β1 and PGE2 and NO production, but did not affect fungicidal activity of monocytes against Pb18. The modulatory effect of silibinin on the monocytes inflammatory response against P. brasiliensis might be important to establish an efficient and beneficial immune response of the host against the fungusCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Peraçoli, Maria Terezinha Serrão [UNESP]Universidade Estadual Paulista (Unesp)Castro, Camila Ferreira Bannwart [UNESP]2014-06-11T19:30:56Z2014-06-11T19:30:56Z2009-12-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis63 f.application/pdfCASTRO, Camila Ferreira Bannwart. Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis. 2009. 63 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2009.http://hdl.handle.net/11449/100582000610876bannwart_cf_dr_botib.pdf33004064080P36486557387397806Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-01-15T06:21:13Zoai:repositorio.unesp.br:11449/100582Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:03:22.510843Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis
title Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis
spellingShingle Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis
Castro, Camila Ferreira Bannwart [UNESP]
Imunologia
Agentes anti-inflamatorios
Paracoccidiodomicose
Immunology
title_short Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis
title_full Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis
title_fullStr Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis
title_full_unstemmed Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis
title_sort Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis
author Castro, Camila Ferreira Bannwart [UNESP]
author_facet Castro, Camila Ferreira Bannwart [UNESP]
author_role author
dc.contributor.none.fl_str_mv Peraçoli, Maria Terezinha Serrão [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Castro, Camila Ferreira Bannwart [UNESP]
dc.subject.por.fl_str_mv Imunologia
Agentes anti-inflamatorios
Paracoccidiodomicose
Immunology
topic Imunologia
Agentes anti-inflamatorios
Paracoccidiodomicose
Immunology
description Silibinin is the major active component of silymarin (Silybum marianum), a polyphenolic plant flavonoid that has anti-inflammatory, cytoprotective and anticarcinogenic effects. The modulatory effect of silibinin on monocyte function against Paracoccidioides brasiliensis (Pb18) has not yet been demonstrated. The present study investigated whether the effect of silibinin on NF-kB pathways may affects the production of tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), Transforming growth factor beta (TGF-β1), prostaglandin E2 (PGE2) and nitric oxide (NO), as well as the fungicidal activity of human monocytes challenged in vitro with Pb18. Peripheral blood monocytes from healthy individuals were treated with or without 5 uM and 50 uM silibinin and challenged with Pb18 or were stimulated with lipopolissaccharide (LPS). After 18 h of culture TNF-α, IL-10, TGF-β1 and PGE2 were determined by ELISA, while NO release was quantified by the accumulation of nitrite in the supernatants using the standard Griess assay. Fungicidal activity of monocytes against Pb18 was assessed after cell incubation with interferon-gamma, culture with or without silibinin for 18 h and challenge with Pb18 for 4h. NF-kB activation in the cultures was evaluated by flow cytometry and ELISA after monocyte stimulation with Pb18 or LPS for 30 min. Silibinin at 50uM concentration partially inhibited p65NF-kB activation as observed by reduction in the number of cells expressing this factor, that was confirmed by low concentration detection of p65NF-kB in the nucleus. This silibinin effects resulted in suppression of TNF-α, IL-10, TGF-β1 and PGE2 and NO production, but did not affect fungicidal activity of monocytes against Pb18. The modulatory effect of silibinin on the monocytes inflammatory response against P. brasiliensis might be important to establish an efficient and beneficial immune response of the host against the fungus
publishDate 2009
dc.date.none.fl_str_mv 2009-12-18
2014-06-11T19:30:56Z
2014-06-11T19:30:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv CASTRO, Camila Ferreira Bannwart. Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis. 2009. 63 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2009.
http://hdl.handle.net/11449/100582
000610876
bannwart_cf_dr_botib.pdf
33004064080P3
6486557387397806
identifier_str_mv CASTRO, Camila Ferreira Bannwart. Avaliação do efeito anti-inflamatório da silibinina sobre monócitos de sangue periférico de indivíduos saudáveis, infectados in vitro com cepa virulenta de Paracoccidioides brasiliensis. 2009. 63 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2009.
000610876
bannwart_cf_dr_botib.pdf
33004064080P3
6486557387397806
url http://hdl.handle.net/11449/100582
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 63 f.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
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reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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