Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0037-8682-0526-2018 http://hdl.handle.net/11449/183891 |
Resumo: | AbstractINTRODUCTION: Crotalus envenomations cause serious complications and can be fatal without appropriate treatment. Venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. We comparatively evaluated the local and systemic effects caused by Crotalus durissus terrificus (Cdt), C.d. collilineatus (Cdcolli), and C.d. cascavella (Cdcasc) envenomation.METHODS:Venom chromatography was performed. Proteolytic, phospholipase, and LAAO activities were analyzed. Edema, myotoxicity, hepatotoxicity, nephrotoxicity, and coagulation alterations were evaluated.RESULTS:The venom SDS-PAGE analyses found the presence of convulxin, gyroxin, crotoxin, and crotamine in Cdt and Cdcolli venoms. Crotamine was not present in the Cdcasc venom. Cdt, Cdcollli, and Cdcasc venoms had no proteolytic activity. Only Cdcasc and Cdt venoms had phospholipase activity. LAAO activity was observed in Cdcolli and Cdcasc venoms. Cdcolli and Cdcasc venoms caused 36.7% and 13.3% edema increases, respectively. Cdt venom caused a 10% edema induction compared to those by other venoms. All venoms increased TOTAL-CK, MB-CK, and LDH levels (indicating muscle injury) and ALT, AST, GGT, and ALP levels (markers of liver damage) and were able to induce a neuromuscular blockade. Urea and creatinine levels were also altered in both plasma and urine, indicating kidney damage. Only Cdcolli and Cdcasc venoms increased TAPP and TAP.CONCLUSIONS:Together, these results allow us to draw a distinction between local and systemic effects caused by Crotalus subspecies, highlighting the clinical and biochemical effects produced by their respective venoms. |
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Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss miceCrotalus durissusMyotoxicityNephrotoxicityHepatotoxicityNeurotoxicityEdemaAbstractINTRODUCTION: Crotalus envenomations cause serious complications and can be fatal without appropriate treatment. Venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. We comparatively evaluated the local and systemic effects caused by Crotalus durissus terrificus (Cdt), C.d. collilineatus (Cdcolli), and C.d. cascavella (Cdcasc) envenomation.METHODS:Venom chromatography was performed. Proteolytic, phospholipase, and LAAO activities were analyzed. Edema, myotoxicity, hepatotoxicity, nephrotoxicity, and coagulation alterations were evaluated.RESULTS:The venom SDS-PAGE analyses found the presence of convulxin, gyroxin, crotoxin, and crotamine in Cdt and Cdcolli venoms. Crotamine was not present in the Cdcasc venom. Cdt, Cdcollli, and Cdcasc venoms had no proteolytic activity. Only Cdcasc and Cdt venoms had phospholipase activity. LAAO activity was observed in Cdcolli and Cdcasc venoms. Cdcolli and Cdcasc venoms caused 36.7% and 13.3% edema increases, respectively. Cdt venom caused a 10% edema induction compared to those by other venoms. All venoms increased TOTAL-CK, MB-CK, and LDH levels (indicating muscle injury) and ALT, AST, GGT, and ALP levels (markers of liver damage) and were able to induce a neuromuscular blockade. Urea and creatinine levels were also altered in both plasma and urine, indicating kidney damage. Only Cdcolli and Cdcasc venoms increased TAPP and TAP.CONCLUSIONS:Together, these results allow us to draw a distinction between local and systemic effects caused by Crotalus subspecies, highlighting the clinical and biochemical effects produced by their respective venoms.Fundação Oswaldo Cruz Laboratório de Imunologia Celular Aplicada à SaúdeInstituto Federal de Educação, Ciência e Tecnologia de Mato Grosso Campus São Vicente Centro de Referência de JaciaraUniversidade Federal de Rondônia Departamento de Medicina Centro de Estudos de Biomoléculas Aplicadas à SaúdeCentro Universitário São LucasUniversidade Estadual de São Paulo Instituto de Biociências Departamento de Física e BiofísicaUniversidade Estadual de São Paulo Instituto de Biociências Departamento de FarmacologiaSociedade Brasileira de Medicina Tropical - SBMTFundação Oswaldo Cruz Laboratório de Imunologia Celular Aplicada à SaúdeInstituto Federal de Educação, Ciência e Tecnologia de Mato Grosso Campus São Vicente Centro de Referência de JaciaraUniversidade Federal de Rondônia Departamento de Medicina Centro de Estudos de Biomoléculas Aplicadas à SaúdeCentro Universitário São LucasUniversidade Estadual de São Paulo Instituto de Biociências Departamento de Física e BiofísicaUniversidade Estadual de São Paulo Instituto de Biociências Departamento de FarmacologiaCarvalho, Letícia Helena DeTeixeira, Leda FabiélenZaqueo, Kayena DelaixBastos, Jéssica FelixNery, Neriane MonteiroSetúbal, Sulamita SilvaPontes, Adriana SilvaButzke, DianaCavalcante, WalterGallacci, MarciaFernandes, Carla Freire CeledônioStabeli, Rodrigo GuerinoSoares, Andreimar MartinsZuliani, Juliana Pavan2019-10-03T17:32:08Z2019-10-03T17:32:08Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/0037-8682-0526-2018Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 52, p. -, 2019.0037-8682http://hdl.handle.net/11449/18389110.1590/0037-8682-0526-2018S0037-86822019000100328S0037-86822019000100328.pdf9353490382598257SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropicalinfo:eu-repo/semantics/openAccess2024-01-21T06:26:18Zoai:repositorio.unesp.br:11449/183891Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:36:42.776408Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice |
title |
Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice |
spellingShingle |
Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice Carvalho, Letícia Helena De Crotalus durissus Myotoxicity Nephrotoxicity Hepatotoxicity Neurotoxicity Edema |
title_short |
Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice |
title_full |
Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice |
title_fullStr |
Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice |
title_full_unstemmed |
Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice |
title_sort |
Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice |
author |
Carvalho, Letícia Helena De |
author_facet |
Carvalho, Letícia Helena De Teixeira, Leda Fabiélen Zaqueo, Kayena Delaix Bastos, Jéssica Felix Nery, Neriane Monteiro Setúbal, Sulamita Silva Pontes, Adriana Silva Butzke, Diana Cavalcante, Walter Gallacci, Marcia Fernandes, Carla Freire Celedônio Stabeli, Rodrigo Guerino Soares, Andreimar Martins Zuliani, Juliana Pavan |
author_role |
author |
author2 |
Teixeira, Leda Fabiélen Zaqueo, Kayena Delaix Bastos, Jéssica Felix Nery, Neriane Monteiro Setúbal, Sulamita Silva Pontes, Adriana Silva Butzke, Diana Cavalcante, Walter Gallacci, Marcia Fernandes, Carla Freire Celedônio Stabeli, Rodrigo Guerino Soares, Andreimar Martins Zuliani, Juliana Pavan |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Fundação Oswaldo Cruz Laboratório de Imunologia Celular Aplicada à Saúde Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso Campus São Vicente Centro de Referência de Jaciara Universidade Federal de Rondônia Departamento de Medicina Centro de Estudos de Biomoléculas Aplicadas à Saúde Centro Universitário São Lucas Universidade Estadual de São Paulo Instituto de Biociências Departamento de Física e Biofísica Universidade Estadual de São Paulo Instituto de Biociências Departamento de Farmacologia |
dc.contributor.author.fl_str_mv |
Carvalho, Letícia Helena De Teixeira, Leda Fabiélen Zaqueo, Kayena Delaix Bastos, Jéssica Felix Nery, Neriane Monteiro Setúbal, Sulamita Silva Pontes, Adriana Silva Butzke, Diana Cavalcante, Walter Gallacci, Marcia Fernandes, Carla Freire Celedônio Stabeli, Rodrigo Guerino Soares, Andreimar Martins Zuliani, Juliana Pavan |
dc.subject.por.fl_str_mv |
Crotalus durissus Myotoxicity Nephrotoxicity Hepatotoxicity Neurotoxicity Edema |
topic |
Crotalus durissus Myotoxicity Nephrotoxicity Hepatotoxicity Neurotoxicity Edema |
description |
AbstractINTRODUCTION: Crotalus envenomations cause serious complications and can be fatal without appropriate treatment. Venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. We comparatively evaluated the local and systemic effects caused by Crotalus durissus terrificus (Cdt), C.d. collilineatus (Cdcolli), and C.d. cascavella (Cdcasc) envenomation.METHODS:Venom chromatography was performed. Proteolytic, phospholipase, and LAAO activities were analyzed. Edema, myotoxicity, hepatotoxicity, nephrotoxicity, and coagulation alterations were evaluated.RESULTS:The venom SDS-PAGE analyses found the presence of convulxin, gyroxin, crotoxin, and crotamine in Cdt and Cdcolli venoms. Crotamine was not present in the Cdcasc venom. Cdt, Cdcollli, and Cdcasc venoms had no proteolytic activity. Only Cdcasc and Cdt venoms had phospholipase activity. LAAO activity was observed in Cdcolli and Cdcasc venoms. Cdcolli and Cdcasc venoms caused 36.7% and 13.3% edema increases, respectively. Cdt venom caused a 10% edema induction compared to those by other venoms. All venoms increased TOTAL-CK, MB-CK, and LDH levels (indicating muscle injury) and ALT, AST, GGT, and ALP levels (markers of liver damage) and were able to induce a neuromuscular blockade. Urea and creatinine levels were also altered in both plasma and urine, indicating kidney damage. Only Cdcolli and Cdcasc venoms increased TAPP and TAP.CONCLUSIONS:Together, these results allow us to draw a distinction between local and systemic effects caused by Crotalus subspecies, highlighting the clinical and biochemical effects produced by their respective venoms. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-03T17:32:08Z 2019-10-03T17:32:08Z 2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-0526-2018 Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 52, p. -, 2019. 0037-8682 http://hdl.handle.net/11449/183891 10.1590/0037-8682-0526-2018 S0037-86822019000100328 S0037-86822019000100328.pdf 9353490382598257 |
url |
http://dx.doi.org/10.1590/0037-8682-0526-2018 http://hdl.handle.net/11449/183891 |
identifier_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 52, p. -, 2019. 0037-8682 10.1590/0037-8682-0526-2018 S0037-86822019000100328 S0037-86822019000100328.pdf 9353490382598257 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129536711196672 |