Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice

Detalhes bibliográficos
Autor(a) principal: Carvalho, Letícia Helena De
Data de Publicação: 2019
Outros Autores: Teixeira, Leda Fabiélen, Zaqueo, Kayena Delaix, Bastos, Jéssica Felix, Nery, Neriane Monteiro, Setúbal, Sulamita Silva, Pontes, Adriana Silva, Butzke, Diana, Cavalcante, Walter, Gallacci, Marcia, Fernandes, Carla Freire Celedônio, Stabeli, Rodrigo Guerino, Soares, Andreimar Martins, Zuliani, Juliana Pavan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0037-8682-0526-2018
http://hdl.handle.net/11449/183891
Resumo: AbstractINTRODUCTION: Crotalus envenomations cause serious complications and can be fatal without appropriate treatment. Venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. We comparatively evaluated the local and systemic effects caused by Crotalus durissus terrificus (Cdt), C.d. collilineatus (Cdcolli), and C.d. cascavella (Cdcasc) envenomation.METHODS:Venom chromatography was performed. Proteolytic, phospholipase, and LAAO activities were analyzed. Edema, myotoxicity, hepatotoxicity, nephrotoxicity, and coagulation alterations were evaluated.RESULTS:The venom SDS-PAGE analyses found the presence of convulxin, gyroxin, crotoxin, and crotamine in Cdt and Cdcolli venoms. Crotamine was not present in the Cdcasc venom. Cdt, Cdcollli, and Cdcasc venoms had no proteolytic activity. Only Cdcasc and Cdt venoms had phospholipase activity. LAAO activity was observed in Cdcolli and Cdcasc venoms. Cdcolli and Cdcasc venoms caused 36.7% and 13.3% edema increases, respectively. Cdt venom caused a 10% edema induction compared to those by other venoms. All venoms increased TOTAL-CK, MB-CK, and LDH levels (indicating muscle injury) and ALT, AST, GGT, and ALP levels (markers of liver damage) and were able to induce a neuromuscular blockade. Urea and creatinine levels were also altered in both plasma and urine, indicating kidney damage. Only Cdcolli and Cdcasc venoms increased TAPP and TAP.CONCLUSIONS:Together, these results allow us to draw a distinction between local and systemic effects caused by Crotalus subspecies, highlighting the clinical and biochemical effects produced by their respective venoms.
id UNSP_77b03a11776bd483f6bac0fa9d86daf0
oai_identifier_str oai:repositorio.unesp.br:11449/183891
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss miceCrotalus durissusMyotoxicityNephrotoxicityHepatotoxicityNeurotoxicityEdemaAbstractINTRODUCTION: Crotalus envenomations cause serious complications and can be fatal without appropriate treatment. Venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. We comparatively evaluated the local and systemic effects caused by Crotalus durissus terrificus (Cdt), C.d. collilineatus (Cdcolli), and C.d. cascavella (Cdcasc) envenomation.METHODS:Venom chromatography was performed. Proteolytic, phospholipase, and LAAO activities were analyzed. Edema, myotoxicity, hepatotoxicity, nephrotoxicity, and coagulation alterations were evaluated.RESULTS:The venom SDS-PAGE analyses found the presence of convulxin, gyroxin, crotoxin, and crotamine in Cdt and Cdcolli venoms. Crotamine was not present in the Cdcasc venom. Cdt, Cdcollli, and Cdcasc venoms had no proteolytic activity. Only Cdcasc and Cdt venoms had phospholipase activity. LAAO activity was observed in Cdcolli and Cdcasc venoms. Cdcolli and Cdcasc venoms caused 36.7% and 13.3% edema increases, respectively. Cdt venom caused a 10% edema induction compared to those by other venoms. All venoms increased TOTAL-CK, MB-CK, and LDH levels (indicating muscle injury) and ALT, AST, GGT, and ALP levels (markers of liver damage) and were able to induce a neuromuscular blockade. Urea and creatinine levels were also altered in both plasma and urine, indicating kidney damage. Only Cdcolli and Cdcasc venoms increased TAPP and TAP.CONCLUSIONS:Together, these results allow us to draw a distinction between local and systemic effects caused by Crotalus subspecies, highlighting the clinical and biochemical effects produced by their respective venoms.Fundação Oswaldo Cruz Laboratório de Imunologia Celular Aplicada à SaúdeInstituto Federal de Educação, Ciência e Tecnologia de Mato Grosso Campus São Vicente Centro de Referência de JaciaraUniversidade Federal de Rondônia Departamento de Medicina Centro de Estudos de Biomoléculas Aplicadas à SaúdeCentro Universitário São LucasUniversidade Estadual de São Paulo Instituto de Biociências Departamento de Física e BiofísicaUniversidade Estadual de São Paulo Instituto de Biociências Departamento de FarmacologiaSociedade Brasileira de Medicina Tropical - SBMTFundação Oswaldo Cruz Laboratório de Imunologia Celular Aplicada à SaúdeInstituto Federal de Educação, Ciência e Tecnologia de Mato Grosso Campus São Vicente Centro de Referência de JaciaraUniversidade Federal de Rondônia Departamento de Medicina Centro de Estudos de Biomoléculas Aplicadas à SaúdeCentro Universitário São LucasUniversidade Estadual de São Paulo Instituto de Biociências Departamento de Física e BiofísicaUniversidade Estadual de São Paulo Instituto de Biociências Departamento de FarmacologiaCarvalho, Letícia Helena DeTeixeira, Leda FabiélenZaqueo, Kayena DelaixBastos, Jéssica FelixNery, Neriane MonteiroSetúbal, Sulamita SilvaPontes, Adriana SilvaButzke, DianaCavalcante, WalterGallacci, MarciaFernandes, Carla Freire CeledônioStabeli, Rodrigo GuerinoSoares, Andreimar MartinsZuliani, Juliana Pavan2019-10-03T17:32:08Z2019-10-03T17:32:08Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/0037-8682-0526-2018Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 52, p. -, 2019.0037-8682http://hdl.handle.net/11449/18389110.1590/0037-8682-0526-2018S0037-86822019000100328S0037-86822019000100328.pdf9353490382598257SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropicalinfo:eu-repo/semantics/openAccess2024-01-21T06:26:18Zoai:repositorio.unesp.br:11449/183891Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:36:42.776408Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
title Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
spellingShingle Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
Carvalho, Letícia Helena De
Crotalus durissus
Myotoxicity
Nephrotoxicity
Hepatotoxicity
Neurotoxicity
Edema
title_short Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
title_full Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
title_fullStr Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
title_full_unstemmed Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
title_sort Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
author Carvalho, Letícia Helena De
author_facet Carvalho, Letícia Helena De
Teixeira, Leda Fabiélen
Zaqueo, Kayena Delaix
Bastos, Jéssica Felix
Nery, Neriane Monteiro
Setúbal, Sulamita Silva
Pontes, Adriana Silva
Butzke, Diana
Cavalcante, Walter
Gallacci, Marcia
Fernandes, Carla Freire Celedônio
Stabeli, Rodrigo Guerino
Soares, Andreimar Martins
Zuliani, Juliana Pavan
author_role author
author2 Teixeira, Leda Fabiélen
Zaqueo, Kayena Delaix
Bastos, Jéssica Felix
Nery, Neriane Monteiro
Setúbal, Sulamita Silva
Pontes, Adriana Silva
Butzke, Diana
Cavalcante, Walter
Gallacci, Marcia
Fernandes, Carla Freire Celedônio
Stabeli, Rodrigo Guerino
Soares, Andreimar Martins
Zuliani, Juliana Pavan
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação Oswaldo Cruz Laboratório de Imunologia Celular Aplicada à Saúde
Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso Campus São Vicente Centro de Referência de Jaciara
Universidade Federal de Rondônia Departamento de Medicina Centro de Estudos de Biomoléculas Aplicadas à Saúde
Centro Universitário São Lucas
Universidade Estadual de São Paulo Instituto de Biociências Departamento de Física e Biofísica
Universidade Estadual de São Paulo Instituto de Biociências Departamento de Farmacologia
dc.contributor.author.fl_str_mv Carvalho, Letícia Helena De
Teixeira, Leda Fabiélen
Zaqueo, Kayena Delaix
Bastos, Jéssica Felix
Nery, Neriane Monteiro
Setúbal, Sulamita Silva
Pontes, Adriana Silva
Butzke, Diana
Cavalcante, Walter
Gallacci, Marcia
Fernandes, Carla Freire Celedônio
Stabeli, Rodrigo Guerino
Soares, Andreimar Martins
Zuliani, Juliana Pavan
dc.subject.por.fl_str_mv Crotalus durissus
Myotoxicity
Nephrotoxicity
Hepatotoxicity
Neurotoxicity
Edema
topic Crotalus durissus
Myotoxicity
Nephrotoxicity
Hepatotoxicity
Neurotoxicity
Edema
description AbstractINTRODUCTION: Crotalus envenomations cause serious complications and can be fatal without appropriate treatment. Venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. We comparatively evaluated the local and systemic effects caused by Crotalus durissus terrificus (Cdt), C.d. collilineatus (Cdcolli), and C.d. cascavella (Cdcasc) envenomation.METHODS:Venom chromatography was performed. Proteolytic, phospholipase, and LAAO activities were analyzed. Edema, myotoxicity, hepatotoxicity, nephrotoxicity, and coagulation alterations were evaluated.RESULTS:The venom SDS-PAGE analyses found the presence of convulxin, gyroxin, crotoxin, and crotamine in Cdt and Cdcolli venoms. Crotamine was not present in the Cdcasc venom. Cdt, Cdcollli, and Cdcasc venoms had no proteolytic activity. Only Cdcasc and Cdt venoms had phospholipase activity. LAAO activity was observed in Cdcolli and Cdcasc venoms. Cdcolli and Cdcasc venoms caused 36.7% and 13.3% edema increases, respectively. Cdt venom caused a 10% edema induction compared to those by other venoms. All venoms increased TOTAL-CK, MB-CK, and LDH levels (indicating muscle injury) and ALT, AST, GGT, and ALP levels (markers of liver damage) and were able to induce a neuromuscular blockade. Urea and creatinine levels were also altered in both plasma and urine, indicating kidney damage. Only Cdcolli and Cdcasc venoms increased TAPP and TAP.CONCLUSIONS:Together, these results allow us to draw a distinction between local and systemic effects caused by Crotalus subspecies, highlighting the clinical and biochemical effects produced by their respective venoms.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-03T17:32:08Z
2019-10-03T17:32:08Z
2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0037-8682-0526-2018
Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 52, p. -, 2019.
0037-8682
http://hdl.handle.net/11449/183891
10.1590/0037-8682-0526-2018
S0037-86822019000100328
S0037-86822019000100328.pdf
9353490382598257
url http://dx.doi.org/10.1590/0037-8682-0526-2018
http://hdl.handle.net/11449/183891
identifier_str_mv Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 52, p. -, 2019.
0037-8682
10.1590/0037-8682-0526-2018
S0037-86822019000100328
S0037-86822019000100328.pdf
9353490382598257
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129536711196672