Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1139/y00-010 http://hdl.handle.net/11449/224158 |
Resumo: | The effect of tetracaine on 45Ca efflux, cytoplasmic Ca2+ concentration [Ca2+](i), and insulin secretion in isolated pancreatic islets and β-cells was studied. In the absence of external Ca2+, tetracaine (0.1-2.0 mM) increased the 45Ca efflux from isolated islets in a dose-dependant manner. Tetracaine did not affect the increase in 45Ca efflux caused by 50 mM K+ or by the association of carbachol (0.2 mM) and 50 mM K+. Tetracaine permanently increased the [Ca2+](i) in isolated β-cells in Ca2+-free medium enriched with 2.8 mM glucose and 25 μM D-600 (methoxiverapamil). This effect was also observed in the presence of 10 mM caffeine or 1 μM thapsigargin. In the presence of 16.7 mM glucose, tetracaine transiently increased the insulin secretion from islets perfused in the absence and presence of external Ca2+. These data indicate that tetracaine mobilises Ca2+ from a thapsigargin-insensitive store and stimulates insulin secretion in the absence of extracellular Ca2+. The increase in 45Ca efflux caused by high concentrations of K+ and by carbachol indicates that tetracaine did not interfere with a cation or inositol triphosphate sensitive Ca2+ pool in β-cells. |
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Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cellsCa2+Insulin secretionTetracaineβ-cellsThe effect of tetracaine on 45Ca efflux, cytoplasmic Ca2+ concentration [Ca2+](i), and insulin secretion in isolated pancreatic islets and β-cells was studied. In the absence of external Ca2+, tetracaine (0.1-2.0 mM) increased the 45Ca efflux from isolated islets in a dose-dependant manner. Tetracaine did not affect the increase in 45Ca efflux caused by 50 mM K+ or by the association of carbachol (0.2 mM) and 50 mM K+. Tetracaine permanently increased the [Ca2+](i) in isolated β-cells in Ca2+-free medium enriched with 2.8 mM glucose and 25 μM D-600 (methoxiverapamil). This effect was also observed in the presence of 10 mM caffeine or 1 μM thapsigargin. In the presence of 16.7 mM glucose, tetracaine transiently increased the insulin secretion from islets perfused in the absence and presence of external Ca2+. These data indicate that tetracaine mobilises Ca2+ from a thapsigargin-insensitive store and stimulates insulin secretion in the absence of extracellular Ca2+. The increase in 45Ca efflux caused by high concentrations of K+ and by carbachol indicates that tetracaine did not interfere with a cation or inositol triphosphate sensitive Ca2+ pool in β-cells.Department of Biological Sciences UNESP, Bauru, SPDept. of Physiology and Biophysics UNICAMP, Campinas, 13083-970, SPDepartment of Biological Sciences UNESP, Bauru, SPUniversidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Bosqueiro, José Roberto [UNESP]Carneiro, Everardo MagalhãesBordin, SilvanaBoschero, Antonio Carlos2022-04-28T19:54:58Z2022-04-28T19:54:58Z2000-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article462-468http://dx.doi.org/10.1139/y00-010Canadian Journal of Physiology and Pharmacology, v. 78, n. 6, p. 462-468, 2000.0008-4212http://hdl.handle.net/11449/22415810.1139/y00-0102-s2.0-0033922419Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal of Physiology and Pharmacologyinfo:eu-repo/semantics/openAccess2024-04-23T15:23:16Zoai:repositorio.unesp.br:11449/224158Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:28:35.775113Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells |
title |
Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells |
spellingShingle |
Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells Bosqueiro, José Roberto [UNESP] Ca2+ Insulin secretion Tetracaine β-cells |
title_short |
Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells |
title_full |
Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells |
title_fullStr |
Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells |
title_full_unstemmed |
Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells |
title_sort |
Tetracaine stimulates insulin secretion through the mobilization of Ca2+ from thapsigargin- and IP3-insensitive Ca2+ reservoir in pancreatic β-cells |
author |
Bosqueiro, José Roberto [UNESP] |
author_facet |
Bosqueiro, José Roberto [UNESP] Carneiro, Everardo Magalhães Bordin, Silvana Boschero, Antonio Carlos |
author_role |
author |
author2 |
Carneiro, Everardo Magalhães Bordin, Silvana Boschero, Antonio Carlos |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Bosqueiro, José Roberto [UNESP] Carneiro, Everardo Magalhães Bordin, Silvana Boschero, Antonio Carlos |
dc.subject.por.fl_str_mv |
Ca2+ Insulin secretion Tetracaine β-cells |
topic |
Ca2+ Insulin secretion Tetracaine β-cells |
description |
The effect of tetracaine on 45Ca efflux, cytoplasmic Ca2+ concentration [Ca2+](i), and insulin secretion in isolated pancreatic islets and β-cells was studied. In the absence of external Ca2+, tetracaine (0.1-2.0 mM) increased the 45Ca efflux from isolated islets in a dose-dependant manner. Tetracaine did not affect the increase in 45Ca efflux caused by 50 mM K+ or by the association of carbachol (0.2 mM) and 50 mM K+. Tetracaine permanently increased the [Ca2+](i) in isolated β-cells in Ca2+-free medium enriched with 2.8 mM glucose and 25 μM D-600 (methoxiverapamil). This effect was also observed in the presence of 10 mM caffeine or 1 μM thapsigargin. In the presence of 16.7 mM glucose, tetracaine transiently increased the insulin secretion from islets perfused in the absence and presence of external Ca2+. These data indicate that tetracaine mobilises Ca2+ from a thapsigargin-insensitive store and stimulates insulin secretion in the absence of extracellular Ca2+. The increase in 45Ca efflux caused by high concentrations of K+ and by carbachol indicates that tetracaine did not interfere with a cation or inositol triphosphate sensitive Ca2+ pool in β-cells. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-01-01 2022-04-28T19:54:58Z 2022-04-28T19:54:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1139/y00-010 Canadian Journal of Physiology and Pharmacology, v. 78, n. 6, p. 462-468, 2000. 0008-4212 http://hdl.handle.net/11449/224158 10.1139/y00-010 2-s2.0-0033922419 |
url |
http://dx.doi.org/10.1139/y00-010 http://hdl.handle.net/11449/224158 |
identifier_str_mv |
Canadian Journal of Physiology and Pharmacology, v. 78, n. 6, p. 462-468, 2000. 0008-4212 10.1139/y00-010 2-s2.0-0033922419 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Canadian Journal of Physiology and Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
462-468 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128658924109824 |