From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/21505594.2017.1397871 http://hdl.handle.net/11449/180180 |
Resumo: | Galleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages of this model include the low cost of maintenance, the fast life cycle, the possibility of using a large number of caterpillars and the innate immune system, which is evolutionarily conserved relative to mammals. Because of these advantages, different research groups have been working to implement the rearing of G. mellonella in laboratory conditions. This protocol describes our experience in the rearing of G. mellonella caterpillars for experimental infection models and the influence of different artificial diets on developmental and physiological parameters. Here, we suggest a diet composition that benefits the life cycle of G. mellonella by accelerating the larval phase length and increasing the caterpillar weight. This diet also stimulated the immune system of G. mellonella by increasing the hemolymph volume and hemocyte concentration. In addition, our rearing protocol generated caterpillars that are more resistant to infection by Staphylococcus aureus, Escherichia coli and Candida albicans. A standard G. mellonella rearing protocol is fundamental to minimize external influences on the results, and this simple and easy protocol can support researchers starting to rear G. mellonella. |
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From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studiesexperimental modelGalleria mellonellain vivo studyrearingGalleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages of this model include the low cost of maintenance, the fast life cycle, the possibility of using a large number of caterpillars and the innate immune system, which is evolutionarily conserved relative to mammals. Because of these advantages, different research groups have been working to implement the rearing of G. mellonella in laboratory conditions. This protocol describes our experience in the rearing of G. mellonella caterpillars for experimental infection models and the influence of different artificial diets on developmental and physiological parameters. Here, we suggest a diet composition that benefits the life cycle of G. mellonella by accelerating the larval phase length and increasing the caterpillar weight. This diet also stimulated the immune system of G. mellonella by increasing the hemolymph volume and hemocyte concentration. In addition, our rearing protocol generated caterpillars that are more resistant to infection by Staphylococcus aureus, Escherichia coli and Candida albicans. A standard G. mellonella rearing protocol is fundamental to minimize external influences on the results, and this simple and easy protocol can support researchers starting to rear G. mellonella.Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Empresa Brasileira de Agropecuária (Embrapa Gado de Leite)Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Jorjão, Adeline L. [UNESP]Oliveira, Luciane D. [UNESP]Scorzoni, Liliana [UNESP]Figueiredo-Godoi, Lívia Mara A [UNESP]Cristina A Prata, MarciaJorge, Antonio Olavo C [UNESP]Junqueira, Juliana C. [UNESP]2018-12-11T17:38:30Z2018-12-11T17:38:30Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article383-389application/pdfhttp://dx.doi.org/10.1080/21505594.2017.1397871Virulence, v. 9, n. 1, p. 383-389, 2018.2150-5608http://hdl.handle.net/11449/18018010.1080/21505594.2017.13978712-s2.0-850533275602-s2.0-85053327560.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirulence1,635info:eu-repo/semantics/openAccess2023-11-10T06:12:15Zoai:repositorio.unesp.br:11449/180180Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:19:36.654278Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies |
title |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies |
spellingShingle |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies Jorjão, Adeline L. [UNESP] experimental model Galleria mellonella in vivo study rearing |
title_short |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies |
title_full |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies |
title_fullStr |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies |
title_full_unstemmed |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies |
title_sort |
From moths to caterpillars: Ideal conditions for Galleria mellonella rearing for in vivo microbiological studies |
author |
Jorjão, Adeline L. [UNESP] |
author_facet |
Jorjão, Adeline L. [UNESP] Oliveira, Luciane D. [UNESP] Scorzoni, Liliana [UNESP] Figueiredo-Godoi, Lívia Mara A [UNESP] Cristina A Prata, Marcia Jorge, Antonio Olavo C [UNESP] Junqueira, Juliana C. [UNESP] |
author_role |
author |
author2 |
Oliveira, Luciane D. [UNESP] Scorzoni, Liliana [UNESP] Figueiredo-Godoi, Lívia Mara A [UNESP] Cristina A Prata, Marcia Jorge, Antonio Olavo C [UNESP] Junqueira, Juliana C. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Jorjão, Adeline L. [UNESP] Oliveira, Luciane D. [UNESP] Scorzoni, Liliana [UNESP] Figueiredo-Godoi, Lívia Mara A [UNESP] Cristina A Prata, Marcia Jorge, Antonio Olavo C [UNESP] Junqueira, Juliana C. [UNESP] |
dc.subject.por.fl_str_mv |
experimental model Galleria mellonella in vivo study rearing |
topic |
experimental model Galleria mellonella in vivo study rearing |
description |
Galleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages of this model include the low cost of maintenance, the fast life cycle, the possibility of using a large number of caterpillars and the innate immune system, which is evolutionarily conserved relative to mammals. Because of these advantages, different research groups have been working to implement the rearing of G. mellonella in laboratory conditions. This protocol describes our experience in the rearing of G. mellonella caterpillars for experimental infection models and the influence of different artificial diets on developmental and physiological parameters. Here, we suggest a diet composition that benefits the life cycle of G. mellonella by accelerating the larval phase length and increasing the caterpillar weight. This diet also stimulated the immune system of G. mellonella by increasing the hemolymph volume and hemocyte concentration. In addition, our rearing protocol generated caterpillars that are more resistant to infection by Staphylococcus aureus, Escherichia coli and Candida albicans. A standard G. mellonella rearing protocol is fundamental to minimize external influences on the results, and this simple and easy protocol can support researchers starting to rear G. mellonella. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:38:30Z 2018-12-11T17:38:30Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/21505594.2017.1397871 Virulence, v. 9, n. 1, p. 383-389, 2018. 2150-5608 http://hdl.handle.net/11449/180180 10.1080/21505594.2017.1397871 2-s2.0-85053327560 2-s2.0-85053327560.pdf |
url |
http://dx.doi.org/10.1080/21505594.2017.1397871 http://hdl.handle.net/11449/180180 |
identifier_str_mv |
Virulence, v. 9, n. 1, p. 383-389, 2018. 2150-5608 10.1080/21505594.2017.1397871 2-s2.0-85053327560 2-s2.0-85053327560.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Virulence 1,635 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
383-389 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128790600089600 |