Exploring Folding Aspects of Monomeric Superoxide Dismutase
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acs.jpcb.9b09640 http://hdl.handle.net/11449/201509 |
Resumo: | Recent studies have associated the absence of bound metals (Apo protein) and mutations in Cu-Zn Human Superoxide Dismutase (SOD1) with amyotrophic lateral sclerosis (ALS) disease, suggesting mechanisms of SOD1 aggregation. Using a structure-based model and modifying the energy of interaction between amino acids in the metal-binding site, we detected differences between the folding of the apo and holo proteins. The presence of metal ions decreases the free-energy barrier and also suggests that the folding pathway may change to reach the native state. The kinetics of folding of the apo and holo forms also correlates with the amount of free-energy barrier in the folding process. Also, the stability of the native state is significantly affected by the absence of metal ions. Our results, obtained from a very simplified model, correlate with more detailed studies, which also have shown that the transition and the native states are affected by the absence of the metal ions, hindering the folding of SOD1 and decreasing the stability of the native state. Regarding the disulfide bond, the results show that its absence decreases the stability of the native structure but affects the transition state less, suggesting that it is possibly made late in the folding process. |
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Exploring Folding Aspects of Monomeric Superoxide DismutaseRecent studies have associated the absence of bound metals (Apo protein) and mutations in Cu-Zn Human Superoxide Dismutase (SOD1) with amyotrophic lateral sclerosis (ALS) disease, suggesting mechanisms of SOD1 aggregation. Using a structure-based model and modifying the energy of interaction between amino acids in the metal-binding site, we detected differences between the folding of the apo and holo proteins. The presence of metal ions decreases the free-energy barrier and also suggests that the folding pathway may change to reach the native state. The kinetics of folding of the apo and holo forms also correlates with the amount of free-energy barrier in the folding process. Also, the stability of the native state is significantly affected by the absence of metal ions. Our results, obtained from a very simplified model, correlate with more detailed studies, which also have shown that the transition and the native states are affected by the absence of the metal ions, hindering the folding of SOD1 and decreasing the stability of the native state. Regarding the disulfide bond, the results show that its absence decreases the stability of the native structure but affects the transition state less, suggesting that it is possibly made late in the folding process.São Paulo State University (UNESP) IBILCECenter for Theoretical Biological Physics Rice UniversitySão Paulo State University (UNESP) IBILCEUniversidade Estadual Paulista (Unesp)Rice UniversityMouro, Paulo R. [UNESP]Povinelli, Ana P. R. [UNESP]Leite, Vitor B. P. [UNESP]Chahine, Jorge [UNESP]2020-12-12T02:34:25Z2020-12-12T02:34:25Z2020-01-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article650-661http://dx.doi.org/10.1021/acs.jpcb.9b09640Journal of Physical Chemistry B, v. 124, n. 4, p. 650-661, 2020.1520-52071520-6106http://hdl.handle.net/11449/20150910.1021/acs.jpcb.9b096402-s2.0-85078693653Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Physical Chemistry Binfo:eu-repo/semantics/openAccess2021-10-22T20:04:27Zoai:repositorio.unesp.br:11449/201509Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:04:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exploring Folding Aspects of Monomeric Superoxide Dismutase |
title |
Exploring Folding Aspects of Monomeric Superoxide Dismutase |
spellingShingle |
Exploring Folding Aspects of Monomeric Superoxide Dismutase Mouro, Paulo R. [UNESP] |
title_short |
Exploring Folding Aspects of Monomeric Superoxide Dismutase |
title_full |
Exploring Folding Aspects of Monomeric Superoxide Dismutase |
title_fullStr |
Exploring Folding Aspects of Monomeric Superoxide Dismutase |
title_full_unstemmed |
Exploring Folding Aspects of Monomeric Superoxide Dismutase |
title_sort |
Exploring Folding Aspects of Monomeric Superoxide Dismutase |
author |
Mouro, Paulo R. [UNESP] |
author_facet |
Mouro, Paulo R. [UNESP] Povinelli, Ana P. R. [UNESP] Leite, Vitor B. P. [UNESP] Chahine, Jorge [UNESP] |
author_role |
author |
author2 |
Povinelli, Ana P. R. [UNESP] Leite, Vitor B. P. [UNESP] Chahine, Jorge [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Rice University |
dc.contributor.author.fl_str_mv |
Mouro, Paulo R. [UNESP] Povinelli, Ana P. R. [UNESP] Leite, Vitor B. P. [UNESP] Chahine, Jorge [UNESP] |
description |
Recent studies have associated the absence of bound metals (Apo protein) and mutations in Cu-Zn Human Superoxide Dismutase (SOD1) with amyotrophic lateral sclerosis (ALS) disease, suggesting mechanisms of SOD1 aggregation. Using a structure-based model and modifying the energy of interaction between amino acids in the metal-binding site, we detected differences between the folding of the apo and holo proteins. The presence of metal ions decreases the free-energy barrier and also suggests that the folding pathway may change to reach the native state. The kinetics of folding of the apo and holo forms also correlates with the amount of free-energy barrier in the folding process. Also, the stability of the native state is significantly affected by the absence of metal ions. Our results, obtained from a very simplified model, correlate with more detailed studies, which also have shown that the transition and the native states are affected by the absence of the metal ions, hindering the folding of SOD1 and decreasing the stability of the native state. Regarding the disulfide bond, the results show that its absence decreases the stability of the native structure but affects the transition state less, suggesting that it is possibly made late in the folding process. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:34:25Z 2020-12-12T02:34:25Z 2020-01-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acs.jpcb.9b09640 Journal of Physical Chemistry B, v. 124, n. 4, p. 650-661, 2020. 1520-5207 1520-6106 http://hdl.handle.net/11449/201509 10.1021/acs.jpcb.9b09640 2-s2.0-85078693653 |
url |
http://dx.doi.org/10.1021/acs.jpcb.9b09640 http://hdl.handle.net/11449/201509 |
identifier_str_mv |
Journal of Physical Chemistry B, v. 124, n. 4, p. 650-661, 2020. 1520-5207 1520-6106 10.1021/acs.jpcb.9b09640 2-s2.0-85078693653 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Physical Chemistry B |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
650-661 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046447022604288 |