Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/sj.thj.6200209 http://hdl.handle.net/11449/230932 |
Resumo: | Introduction: Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that nonspecific esterases from normal monocytes can be determined by a chemiluminescent method. In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories. Materials and Methods: Bone marrow samples were obtained from 66 patients with AML (M0, two cases; M1, 12 cases; M2, 13 cases; M3, 10 cases; M4, 11 cases; M5, 12 cases; M6, two cases; M7, four cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured for 10min. Two parameters were assessed, the peak (PLE) and the integrated light emission (ILE). Results: Both PLE and ILE were higher in FAB-M4 and FAB-M5 subtypes compared to other AML subtypes (P < 0.001). In addition, the classification of AML cases into FAB-M4, FAB-M5 and nonmonocytic subtypes based on ILE analysis was concordant with α-naphthyl acetate esterase (ANAE) in 97% of cases (kappa coefficient 0.94, P < 0.001). Conclusions: These findings indicate that this chemiluminescent assay was able to determine the monocytic component of FAB-M4 and FAB-M5 cells, and the classification of AML subtypes based on chemiluminescent analysis strongly agreed with the cytochemical ANAE-staining. In conclusion, this chemiluminescent assay is a simple, fast and objective method, which may be useful as an alternative tool in the differential diagnosis of AML subtypes. |
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Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assayAcute myeloid leukemiaChemiluminescenceDiagnosisEsteraseMonocyteIntroduction: Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that nonspecific esterases from normal monocytes can be determined by a chemiluminescent method. In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories. Materials and Methods: Bone marrow samples were obtained from 66 patients with AML (M0, two cases; M1, 12 cases; M2, 13 cases; M3, 10 cases; M4, 11 cases; M5, 12 cases; M6, two cases; M7, four cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured for 10min. Two parameters were assessed, the peak (PLE) and the integrated light emission (ILE). Results: Both PLE and ILE were higher in FAB-M4 and FAB-M5 subtypes compared to other AML subtypes (P < 0.001). In addition, the classification of AML cases into FAB-M4, FAB-M5 and nonmonocytic subtypes based on ILE analysis was concordant with α-naphthyl acetate esterase (ANAE) in 97% of cases (kappa coefficient 0.94, P < 0.001). Conclusions: These findings indicate that this chemiluminescent assay was able to determine the monocytic component of FAB-M4 and FAB-M5 cells, and the classification of AML subtypes based on chemiluminescent analysis strongly agreed with the cytochemical ANAE-staining. In conclusion, this chemiluminescent assay is a simple, fast and objective method, which may be useful as an alternative tool in the differential diagnosis of AML subtypes.Department of Clinical Analysis State Univ. of São Paulo School of Pharmaceutical Science, AraraquaraDepartment of Biochemistry Univ. of Sao Paulo Chem. Inst., São PauloCenter for Cell-based Therapy Department of Clinical Medicine Univ. S. Paulo at R. Preto Med. Sch., Av. Bandeirantes 3900, Ribeirão Preto, 14049-900 SPUniversidade de São Paulo (USP)Univ. S. Paulo at R. Preto Med. Sch.da Fonseca, Luiz M.Brunetti, Iguatemy L.Campa, AnaCatalani, Luiz H.Calado, Rodrigo T.Falcão, Roberto Passetto2022-04-29T08:42:48Z2022-04-29T08:42:48Z2003-08-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article26-30http://dx.doi.org/10.1038/sj.thj.6200209Hematology Journal, v. 4, n. 1, p. 26-30, 2003.1466-4860http://hdl.handle.net/11449/23093210.1038/sj.thj.62002092-s2.0-0038605532Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHematology Journalinfo:eu-repo/semantics/openAccess2024-06-21T15:18:58Zoai:repositorio.unesp.br:11449/230932Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:17:21.430293Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay |
title |
Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay |
spellingShingle |
Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay da Fonseca, Luiz M. Acute myeloid leukemia Chemiluminescence Diagnosis Esterase Monocyte |
title_short |
Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay |
title_full |
Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay |
title_fullStr |
Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay |
title_full_unstemmed |
Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay |
title_sort |
Assessment of monocytic component in acute myelomonocytic and monocytic/monoblastic leukemias by a chemiluminescent assay |
author |
da Fonseca, Luiz M. |
author_facet |
da Fonseca, Luiz M. Brunetti, Iguatemy L. Campa, Ana Catalani, Luiz H. Calado, Rodrigo T. Falcão, Roberto Passetto |
author_role |
author |
author2 |
Brunetti, Iguatemy L. Campa, Ana Catalani, Luiz H. Calado, Rodrigo T. Falcão, Roberto Passetto |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Univ. S. Paulo at R. Preto Med. Sch. |
dc.contributor.author.fl_str_mv |
da Fonseca, Luiz M. Brunetti, Iguatemy L. Campa, Ana Catalani, Luiz H. Calado, Rodrigo T. Falcão, Roberto Passetto |
dc.subject.por.fl_str_mv |
Acute myeloid leukemia Chemiluminescence Diagnosis Esterase Monocyte |
topic |
Acute myeloid leukemia Chemiluminescence Diagnosis Esterase Monocyte |
description |
Introduction: Classically, the monocytic component of acute myelomonocytic (FAB-M4) and acute monocytic/monoblastic (FAB-M5) leukemias is demonstrated by nonspecific esterase positivity in cytochemical stainings. We have previously demonstrated that nonspecific esterases from normal monocytes can be determined by a chemiluminescent method. In the present study, we investigated whether this assay can also determine the monocytic component of FAB-M4 and FAB-M5 and distinguish these acute myeloid leukemia (AML) categories. Materials and Methods: Bone marrow samples were obtained from 66 patients with AML (M0, two cases; M1, 12 cases; M2, 13 cases; M3, 10 cases; M4, 11 cases; M5, 12 cases; M6, two cases; M7, four cases). Cells were incubated with a standard reaction mixture and chemiluminescence was measured for 10min. Two parameters were assessed, the peak (PLE) and the integrated light emission (ILE). Results: Both PLE and ILE were higher in FAB-M4 and FAB-M5 subtypes compared to other AML subtypes (P < 0.001). In addition, the classification of AML cases into FAB-M4, FAB-M5 and nonmonocytic subtypes based on ILE analysis was concordant with α-naphthyl acetate esterase (ANAE) in 97% of cases (kappa coefficient 0.94, P < 0.001). Conclusions: These findings indicate that this chemiluminescent assay was able to determine the monocytic component of FAB-M4 and FAB-M5 cells, and the classification of AML subtypes based on chemiluminescent analysis strongly agreed with the cytochemical ANAE-staining. In conclusion, this chemiluminescent assay is a simple, fast and objective method, which may be useful as an alternative tool in the differential diagnosis of AML subtypes. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-08-11 2022-04-29T08:42:48Z 2022-04-29T08:42:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/sj.thj.6200209 Hematology Journal, v. 4, n. 1, p. 26-30, 2003. 1466-4860 http://hdl.handle.net/11449/230932 10.1038/sj.thj.6200209 2-s2.0-0038605532 |
url |
http://dx.doi.org/10.1038/sj.thj.6200209 http://hdl.handle.net/11449/230932 |
identifier_str_mv |
Hematology Journal, v. 4, n. 1, p. 26-30, 2003. 1466-4860 10.1038/sj.thj.6200209 2-s2.0-0038605532 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hematology Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
26-30 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129047243259904 |