Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bjoms.2016.02.018 http://hdl.handle.net/11449/173116 |
Resumo: | Immunohistochemical studies and molecular biology have enabled us to identify numerous proteins that are involved in the metabolism of bone, and their encoding genes. Among these is alkaline phosphatase (ALP), an enzyme that is responsible for the initiation of mineralisation of the extracellular matrix during alveolar bone repair. To evaluate the gene expression of ALP during this process, we studied nine healthy adult male rats, which had their maxillary central incisors extracted from the right side and were randomly divided into three groups. During three experimental periods, 7 days, 14 days, and 28 days, the alveoli were curetted, the rats killed, and samples analysed by real-time reverse transcription polymerase chain reaction (qRT-PCR). The RNAm that encodes the gene for the synthesis of ALP was expressed during the three periods analysed, but its concentration was significantly increased at 14 and 28 days compared with at 7 days. There was no significant difference between 14 and 28 days (p = 0.0005). We conclude that genes related to ALP are expressed throughout the healing process and more intensively during the later periods (14 and 28 days), which coincides with the increased formation of mineralised bone. |
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Repositório Institucional da UNESP |
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Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in ratsalkaline phosphatasealveolar bone repairbone biologygene expressionmineralizationtooth socketImmunohistochemical studies and molecular biology have enabled us to identify numerous proteins that are involved in the metabolism of bone, and their encoding genes. Among these is alkaline phosphatase (ALP), an enzyme that is responsible for the initiation of mineralisation of the extracellular matrix during alveolar bone repair. To evaluate the gene expression of ALP during this process, we studied nine healthy adult male rats, which had their maxillary central incisors extracted from the right side and were randomly divided into three groups. During three experimental periods, 7 days, 14 days, and 28 days, the alveoli were curetted, the rats killed, and samples analysed by real-time reverse transcription polymerase chain reaction (qRT-PCR). The RNAm that encodes the gene for the synthesis of ALP was expressed during the three periods analysed, but its concentration was significantly increased at 14 and 28 days compared with at 7 days. There was no significant difference between 14 and 28 days (p = 0.0005). We conclude that genes related to ALP are expressed throughout the healing process and more intensively during the later periods (14 and 28 days), which coincides with the increased formation of mineralised bone.Surgery and Integrated Clinic Department Araçatuba Dental School São Paulo State University Júlio de Mesquita Filho (UNESP), Rua José Bonifácio, 1193Basic Sciences Department Araçatuba Dental School São Paulo State University Júlio de Mesquita Filho (UNESP)Surgery and Integrated Clinic Department Araçatuba Dental School São Paulo State University Júlio de Mesquita Filho (UNESP), Rua José Bonifácio, 1193Basic Sciences Department Araçatuba Dental School São Paulo State University Júlio de Mesquita Filho (UNESP)Universidade Estadual Paulista (Unesp)Rodrigues, Willian Caetano [UNESP]Fabris, André Luís Da Silva [UNESP]Hassumi, Jaqueline Suemi [UNESP]Gonçalves, Alaíde [UNESP]Sonoda, Celso Koogi [UNESP]Okamoto, Roberta [UNESP]2018-12-11T17:03:42Z2018-12-11T17:03:42Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article531-535application/pdfhttp://dx.doi.org/10.1016/j.bjoms.2016.02.018British Journal of Oral and Maxillofacial Surgery, v. 54, n. 5, p. 531-535, 2016.1532-19400266-4356http://hdl.handle.net/11449/17311610.1016/j.bjoms.2016.02.0182-s2.0-849757066222-s2.0-84975706622.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBritish Journal of Oral and Maxillofacial Surgery0,687info:eu-repo/semantics/openAccess2023-11-29T06:16:46Zoai:repositorio.unesp.br:11449/173116Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:05:11.840834Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats |
title |
Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats |
spellingShingle |
Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats Rodrigues, Willian Caetano [UNESP] alkaline phosphatase alveolar bone repair bone biology gene expression mineralization tooth socket |
title_short |
Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats |
title_full |
Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats |
title_fullStr |
Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats |
title_full_unstemmed |
Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats |
title_sort |
Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats |
author |
Rodrigues, Willian Caetano [UNESP] |
author_facet |
Rodrigues, Willian Caetano [UNESP] Fabris, André Luís Da Silva [UNESP] Hassumi, Jaqueline Suemi [UNESP] Gonçalves, Alaíde [UNESP] Sonoda, Celso Koogi [UNESP] Okamoto, Roberta [UNESP] |
author_role |
author |
author2 |
Fabris, André Luís Da Silva [UNESP] Hassumi, Jaqueline Suemi [UNESP] Gonçalves, Alaíde [UNESP] Sonoda, Celso Koogi [UNESP] Okamoto, Roberta [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rodrigues, Willian Caetano [UNESP] Fabris, André Luís Da Silva [UNESP] Hassumi, Jaqueline Suemi [UNESP] Gonçalves, Alaíde [UNESP] Sonoda, Celso Koogi [UNESP] Okamoto, Roberta [UNESP] |
dc.subject.por.fl_str_mv |
alkaline phosphatase alveolar bone repair bone biology gene expression mineralization tooth socket |
topic |
alkaline phosphatase alveolar bone repair bone biology gene expression mineralization tooth socket |
description |
Immunohistochemical studies and molecular biology have enabled us to identify numerous proteins that are involved in the metabolism of bone, and their encoding genes. Among these is alkaline phosphatase (ALP), an enzyme that is responsible for the initiation of mineralisation of the extracellular matrix during alveolar bone repair. To evaluate the gene expression of ALP during this process, we studied nine healthy adult male rats, which had their maxillary central incisors extracted from the right side and were randomly divided into three groups. During three experimental periods, 7 days, 14 days, and 28 days, the alveoli were curetted, the rats killed, and samples analysed by real-time reverse transcription polymerase chain reaction (qRT-PCR). The RNAm that encodes the gene for the synthesis of ALP was expressed during the three periods analysed, but its concentration was significantly increased at 14 and 28 days compared with at 7 days. There was no significant difference between 14 and 28 days (p = 0.0005). We conclude that genes related to ALP are expressed throughout the healing process and more intensively during the later periods (14 and 28 days), which coincides with the increased formation of mineralised bone. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 2018-12-11T17:03:42Z 2018-12-11T17:03:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bjoms.2016.02.018 British Journal of Oral and Maxillofacial Surgery, v. 54, n. 5, p. 531-535, 2016. 1532-1940 0266-4356 http://hdl.handle.net/11449/173116 10.1016/j.bjoms.2016.02.018 2-s2.0-84975706622 2-s2.0-84975706622.pdf |
url |
http://dx.doi.org/10.1016/j.bjoms.2016.02.018 http://hdl.handle.net/11449/173116 |
identifier_str_mv |
British Journal of Oral and Maxillofacial Surgery, v. 54, n. 5, p. 531-535, 2016. 1532-1940 0266-4356 10.1016/j.bjoms.2016.02.018 2-s2.0-84975706622 2-s2.0-84975706622.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
British Journal of Oral and Maxillofacial Surgery 0,687 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
531-535 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129017685999616 |