Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12906-016-1375-7 http://hdl.handle.net/11449/169052 |
Resumo: | Background: Eugenia spp. are used in popular medicine in the treatment of pain, diabetes, intestinal disorders and cough. The aim of the work is to evaluate, ex vivo and in vivo, the anti-inflammatory activity of the hydroethanolic extracts of the leaves of Eugenia aurata (EA) and Eugenia punicifolia HBK (EP) upon neutrophils. Methods: Ex vivo, isolated human neutrophils were sensitized by Eugenia extracts (0.1-1000 μg/mL) and stimulated by PMA. In these conditions, different neutrophil activities related to inflammatory process were measured: adhesion, degranulation and NET release. Neutrophil viability and tumor line cells were monitored. In vivo, neutrophil influx was evaluated by peritonitis model performed in mice pretreated with different concentrations of Eugenia extracts. Phytochemical profile was assessed by mass spectrometry. Results: Ex vivo, EA and EP (1000 μg/mL) reduced cell adhesion and degranulation, respectively. NET release was inhibited by EA and EP. Anti-inflammatory activities occurred in the absence of cytotoxicity. In vivo, both EA as EP inhibited neutrophil migration. The phytochemical profile revealed that EA contains myricitrin, rutin, quinic acid and quercetin derivatives. EP presents gallic acid, quercetin derivatives, syringic acid, ellagic acid, monogalloyl-glucose, glycosyringic acid, mudanoside B, HHDP glucose isomer and digalloylglucose isomer. EA and EP inhibit neutrophil migration by different pathways. Conclusion: Different chemical compositions may explain the anti-inflammatory effects described herein for EA and EP. Both extracts inhibit NET release but only EA reduces cell adhesion whereas EP decreases elastase secretion. This work contributes to the elucidation of cellular mechanisms related to the anti-inflammatory activity for leaves of E. aurata and E. punicifolia HBK. |
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Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET releaseAdhesionElastaseEugenia aurataEugenia punicifolia (HBK)InflammationNeutrophilsBackground: Eugenia spp. are used in popular medicine in the treatment of pain, diabetes, intestinal disorders and cough. The aim of the work is to evaluate, ex vivo and in vivo, the anti-inflammatory activity of the hydroethanolic extracts of the leaves of Eugenia aurata (EA) and Eugenia punicifolia HBK (EP) upon neutrophils. Methods: Ex vivo, isolated human neutrophils were sensitized by Eugenia extracts (0.1-1000 μg/mL) and stimulated by PMA. In these conditions, different neutrophil activities related to inflammatory process were measured: adhesion, degranulation and NET release. Neutrophil viability and tumor line cells were monitored. In vivo, neutrophil influx was evaluated by peritonitis model performed in mice pretreated with different concentrations of Eugenia extracts. Phytochemical profile was assessed by mass spectrometry. Results: Ex vivo, EA and EP (1000 μg/mL) reduced cell adhesion and degranulation, respectively. NET release was inhibited by EA and EP. Anti-inflammatory activities occurred in the absence of cytotoxicity. In vivo, both EA as EP inhibited neutrophil migration. The phytochemical profile revealed that EA contains myricitrin, rutin, quinic acid and quercetin derivatives. EP presents gallic acid, quercetin derivatives, syringic acid, ellagic acid, monogalloyl-glucose, glycosyringic acid, mudanoside B, HHDP glucose isomer and digalloylglucose isomer. EA and EP inhibit neutrophil migration by different pathways. Conclusion: Different chemical compositions may explain the anti-inflammatory effects described herein for EA and EP. Both extracts inhibit NET release but only EA reduces cell adhesion whereas EP decreases elastase secretion. This work contributes to the elucidation of cellular mechanisms related to the anti-inflammatory activity for leaves of E. aurata and E. punicifolia HBK.Universidade Estadual Paulista -UNESP Departamento de Ci�ncias Biol�gicas Faculdade de Ci�ncias e Letras, Av Dom Ant�nio, 2100, Parque Universit�rioUniversidade Estadual de Campinas (UNICAMP) ThoMSon Laborat�rio de Espectrometria de Massas Instituto de Qu�micaBiol�gicas e Agr�colas UNICAMP Centro Pluridisciplinar de Pesquisas Qu�micasUniversidade Estadual de Campinas (UNICAMP) Faculdade de Ci�ncias Farmac�uticas, P.O. Box 859Universidade Estadual Paulista -UNESP Departamento de Ci�ncias Biol�gicas Faculdade de Ci�ncias e Letras, Av Dom Ant�nio, 2100, Parque Universit�rioUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Costa, M�rian Feliciano [UNESP]Jesus, Tais Iara [UNESP]Lopes, Bruno Rafael Pereira [UNESP]Angolini, C�lio Fernando FigueiredoMontagnolli, Abner [UNESP]Gomes, Lorraine de Paula [UNESP]Pereira, Gabriela Sterle [UNESP]Ruiz, Ana Lucia Tasca GoisCarvalho, Jo�o ErnestoEberlin, Marcos Nogueirados Santos, Catarina [UNESP]Toledo, Karina Alves [UNESP]2018-12-11T16:44:09Z2018-12-11T16:44:09Z2016-10-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12906-016-1375-7BMC Complementary and Alternative Medicine, v. 16, n. 1, 2016.1472-6882http://hdl.handle.net/11449/16905210.1186/s12906-016-1375-72-s2.0-849920627302-s2.0-84992062730.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary and Alternative Medicine0,858info:eu-repo/semantics/openAccess2023-12-11T06:12:31Zoai:repositorio.unesp.br:11449/169052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-11T06:12:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release |
title |
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release |
spellingShingle |
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release Costa, M�rian Feliciano [UNESP] Adhesion Elastase Eugenia aurata Eugenia punicifolia (HBK) Inflammation Neutrophils |
title_short |
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release |
title_full |
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release |
title_fullStr |
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release |
title_full_unstemmed |
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release |
title_sort |
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release |
author |
Costa, M�rian Feliciano [UNESP] |
author_facet |
Costa, M�rian Feliciano [UNESP] Jesus, Tais Iara [UNESP] Lopes, Bruno Rafael Pereira [UNESP] Angolini, C�lio Fernando Figueiredo Montagnolli, Abner [UNESP] Gomes, Lorraine de Paula [UNESP] Pereira, Gabriela Sterle [UNESP] Ruiz, Ana Lucia Tasca Gois Carvalho, Jo�o Ernesto Eberlin, Marcos Nogueira dos Santos, Catarina [UNESP] Toledo, Karina Alves [UNESP] |
author_role |
author |
author2 |
Jesus, Tais Iara [UNESP] Lopes, Bruno Rafael Pereira [UNESP] Angolini, C�lio Fernando Figueiredo Montagnolli, Abner [UNESP] Gomes, Lorraine de Paula [UNESP] Pereira, Gabriela Sterle [UNESP] Ruiz, Ana Lucia Tasca Gois Carvalho, Jo�o Ernesto Eberlin, Marcos Nogueira dos Santos, Catarina [UNESP] Toledo, Karina Alves [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Costa, M�rian Feliciano [UNESP] Jesus, Tais Iara [UNESP] Lopes, Bruno Rafael Pereira [UNESP] Angolini, C�lio Fernando Figueiredo Montagnolli, Abner [UNESP] Gomes, Lorraine de Paula [UNESP] Pereira, Gabriela Sterle [UNESP] Ruiz, Ana Lucia Tasca Gois Carvalho, Jo�o Ernesto Eberlin, Marcos Nogueira dos Santos, Catarina [UNESP] Toledo, Karina Alves [UNESP] |
dc.subject.por.fl_str_mv |
Adhesion Elastase Eugenia aurata Eugenia punicifolia (HBK) Inflammation Neutrophils |
topic |
Adhesion Elastase Eugenia aurata Eugenia punicifolia (HBK) Inflammation Neutrophils |
description |
Background: Eugenia spp. are used in popular medicine in the treatment of pain, diabetes, intestinal disorders and cough. The aim of the work is to evaluate, ex vivo and in vivo, the anti-inflammatory activity of the hydroethanolic extracts of the leaves of Eugenia aurata (EA) and Eugenia punicifolia HBK (EP) upon neutrophils. Methods: Ex vivo, isolated human neutrophils were sensitized by Eugenia extracts (0.1-1000 μg/mL) and stimulated by PMA. In these conditions, different neutrophil activities related to inflammatory process were measured: adhesion, degranulation and NET release. Neutrophil viability and tumor line cells were monitored. In vivo, neutrophil influx was evaluated by peritonitis model performed in mice pretreated with different concentrations of Eugenia extracts. Phytochemical profile was assessed by mass spectrometry. Results: Ex vivo, EA and EP (1000 μg/mL) reduced cell adhesion and degranulation, respectively. NET release was inhibited by EA and EP. Anti-inflammatory activities occurred in the absence of cytotoxicity. In vivo, both EA as EP inhibited neutrophil migration. The phytochemical profile revealed that EA contains myricitrin, rutin, quinic acid and quercetin derivatives. EP presents gallic acid, quercetin derivatives, syringic acid, ellagic acid, monogalloyl-glucose, glycosyringic acid, mudanoside B, HHDP glucose isomer and digalloylglucose isomer. EA and EP inhibit neutrophil migration by different pathways. Conclusion: Different chemical compositions may explain the anti-inflammatory effects described herein for EA and EP. Both extracts inhibit NET release but only EA reduces cell adhesion whereas EP decreases elastase secretion. This work contributes to the elucidation of cellular mechanisms related to the anti-inflammatory activity for leaves of E. aurata and E. punicifolia HBK. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-22 2018-12-11T16:44:09Z 2018-12-11T16:44:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12906-016-1375-7 BMC Complementary and Alternative Medicine, v. 16, n. 1, 2016. 1472-6882 http://hdl.handle.net/11449/169052 10.1186/s12906-016-1375-7 2-s2.0-84992062730 2-s2.0-84992062730.pdf |
url |
http://dx.doi.org/10.1186/s12906-016-1375-7 http://hdl.handle.net/11449/169052 |
identifier_str_mv |
BMC Complementary and Alternative Medicine, v. 16, n. 1, 2016. 1472-6882 10.1186/s12906-016-1375-7 2-s2.0-84992062730 2-s2.0-84992062730.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Complementary and Alternative Medicine 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965243496988672 |