Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

Detalhes bibliográficos
Autor(a) principal: Stafuzza, N. B. [UNESP]
Data de Publicação: 2012
Outros Autores: Abbey, C. A., Gill, C. A., Womack, J. E., Amaral, M. E. J. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4238/2012.May.22.6
http://hdl.handle.net/11449/117557
Resumo: River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 x coverage of the genome. This genomic resource for river buffalo will facilitate further studies in this economically important species allowing for instance, whole genome physical mapping and isolation of genes and gene clusters, contributing to the elucidation of gene organization and identification of regulatory elements.
id UNSP_799634de0bf2d23d3515000773b256e0
oai_identifier_str oai:repositorio.unesp.br:11449/117557
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Construction and preliminary characterization of a river buffalo bacterial artificial chromosome libraryBAC libraryBubalus bubalisCloningPulse-field gel electrophoresisRiver buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 x coverage of the genome. This genomic resource for river buffalo will facilitate further studies in this economically important species allowing for instance, whole genome physical mapping and isolation of genes and gene clusters, contributing to the elucidation of gene organization and identification of regulatory elements.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Sao Paulo Julio de Mesquita Filho, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP, BrazilTexas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USATexas A&M Univ, Dept Vet Pathobiol, College Stn, TX USAUniv Estadual Sao Paulo Julio de Mesquita Filho, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP, BrazilFAPESP: 08/10725-4FAPESP: 06/59606-1FAPESP: 11/02478-0Funpec-editoraUniversidade Estadual Paulista (Unesp)Texas A&M UnivStafuzza, N. B. [UNESP]Abbey, C. A.Gill, C. A.Womack, J. E.Amaral, M. E. J. [UNESP]2015-03-18T15:56:26Z2015-03-18T15:56:26Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3013-3019application/pdfhttp://dx.doi.org/10.4238/2012.May.22.6Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 3, p. 3013-3019, 2012.1676-5680http://hdl.handle.net/11449/11755710.4238/2012.May.22.6WOS:000308817800121WOS000308817800121.pdf0175083848055466Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Research0,439info:eu-repo/semantics/openAccess2023-10-16T06:05:48Zoai:repositorio.unesp.br:11449/117557Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:05:48.165925Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
title Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
spellingShingle Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
Stafuzza, N. B. [UNESP]
BAC library
Bubalus bubalis
Cloning
Pulse-field gel electrophoresis
title_short Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
title_full Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
title_fullStr Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
title_full_unstemmed Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
title_sort Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
author Stafuzza, N. B. [UNESP]
author_facet Stafuzza, N. B. [UNESP]
Abbey, C. A.
Gill, C. A.
Womack, J. E.
Amaral, M. E. J. [UNESP]
author_role author
author2 Abbey, C. A.
Gill, C. A.
Womack, J. E.
Amaral, M. E. J. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Texas A&M Univ
dc.contributor.author.fl_str_mv Stafuzza, N. B. [UNESP]
Abbey, C. A.
Gill, C. A.
Womack, J. E.
Amaral, M. E. J. [UNESP]
dc.subject.por.fl_str_mv BAC library
Bubalus bubalis
Cloning
Pulse-field gel electrophoresis
topic BAC library
Bubalus bubalis
Cloning
Pulse-field gel electrophoresis
description River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 x coverage of the genome. This genomic resource for river buffalo will facilitate further studies in this economically important species allowing for instance, whole genome physical mapping and isolation of genes and gene clusters, contributing to the elucidation of gene organization and identification of regulatory elements.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2015-03-18T15:56:26Z
2015-03-18T15:56:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4238/2012.May.22.6
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 3, p. 3013-3019, 2012.
1676-5680
http://hdl.handle.net/11449/117557
10.4238/2012.May.22.6
WOS:000308817800121
WOS000308817800121.pdf
0175083848055466
url http://dx.doi.org/10.4238/2012.May.22.6
http://hdl.handle.net/11449/117557
identifier_str_mv Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 3, p. 3013-3019, 2012.
1676-5680
10.4238/2012.May.22.6
WOS:000308817800121
WOS000308817800121.pdf
0175083848055466
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics And Molecular Research
0,439
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3013-3019
application/pdf
dc.publisher.none.fl_str_mv Funpec-editora
publisher.none.fl_str_mv Funpec-editora
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128459395825664