Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/2012.May.22.6 http://hdl.handle.net/11449/117557 |
Resumo: | River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 x coverage of the genome. This genomic resource for river buffalo will facilitate further studies in this economically important species allowing for instance, whole genome physical mapping and isolation of genes and gene clusters, contributing to the elucidation of gene organization and identification of regulatory elements. |
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Construction and preliminary characterization of a river buffalo bacterial artificial chromosome libraryBAC libraryBubalus bubalisCloningPulse-field gel electrophoresisRiver buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 x coverage of the genome. This genomic resource for river buffalo will facilitate further studies in this economically important species allowing for instance, whole genome physical mapping and isolation of genes and gene clusters, contributing to the elucidation of gene organization and identification of regulatory elements.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Sao Paulo Julio de Mesquita Filho, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP, BrazilTexas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USATexas A&M Univ, Dept Vet Pathobiol, College Stn, TX USAUniv Estadual Sao Paulo Julio de Mesquita Filho, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP, BrazilFAPESP: 08/10725-4FAPESP: 06/59606-1FAPESP: 11/02478-0Funpec-editoraUniversidade Estadual Paulista (Unesp)Texas A&M UnivStafuzza, N. B. [UNESP]Abbey, C. A.Gill, C. A.Womack, J. E.Amaral, M. E. J. [UNESP]2015-03-18T15:56:26Z2015-03-18T15:56:26Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3013-3019application/pdfhttp://dx.doi.org/10.4238/2012.May.22.6Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 3, p. 3013-3019, 2012.1676-5680http://hdl.handle.net/11449/11755710.4238/2012.May.22.6WOS:000308817800121WOS000308817800121.pdf0175083848055466Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Research0,439info:eu-repo/semantics/openAccess2023-10-16T06:05:48Zoai:repositorio.unesp.br:11449/117557Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:05:48.165925Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library |
title |
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library |
spellingShingle |
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library Stafuzza, N. B. [UNESP] BAC library Bubalus bubalis Cloning Pulse-field gel electrophoresis |
title_short |
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library |
title_full |
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library |
title_fullStr |
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library |
title_full_unstemmed |
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library |
title_sort |
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library |
author |
Stafuzza, N. B. [UNESP] |
author_facet |
Stafuzza, N. B. [UNESP] Abbey, C. A. Gill, C. A. Womack, J. E. Amaral, M. E. J. [UNESP] |
author_role |
author |
author2 |
Abbey, C. A. Gill, C. A. Womack, J. E. Amaral, M. E. J. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Texas A&M Univ |
dc.contributor.author.fl_str_mv |
Stafuzza, N. B. [UNESP] Abbey, C. A. Gill, C. A. Womack, J. E. Amaral, M. E. J. [UNESP] |
dc.subject.por.fl_str_mv |
BAC library Bubalus bubalis Cloning Pulse-field gel electrophoresis |
topic |
BAC library Bubalus bubalis Cloning Pulse-field gel electrophoresis |
description |
River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 x coverage of the genome. This genomic resource for river buffalo will facilitate further studies in this economically important species allowing for instance, whole genome physical mapping and isolation of genes and gene clusters, contributing to the elucidation of gene organization and identification of regulatory elements. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2015-03-18T15:56:26Z 2015-03-18T15:56:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/2012.May.22.6 Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 3, p. 3013-3019, 2012. 1676-5680 http://hdl.handle.net/11449/117557 10.4238/2012.May.22.6 WOS:000308817800121 WOS000308817800121.pdf 0175083848055466 |
url |
http://dx.doi.org/10.4238/2012.May.22.6 http://hdl.handle.net/11449/117557 |
identifier_str_mv |
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 11, n. 3, p. 3013-3019, 2012. 1676-5680 10.4238/2012.May.22.6 WOS:000308817800121 WOS000308817800121.pdf 0175083848055466 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics And Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3013-3019 application/pdf |
dc.publisher.none.fl_str_mv |
Funpec-editora |
publisher.none.fl_str_mv |
Funpec-editora |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128459395825664 |