Leptospira spp. infection in sheep herds in southeast Brazil

Detalhes bibliográficos
Autor(a) principal: Barbante, Priscila [UNESP]
Data de Publicação: 2014
Outros Autores: Shimabukuro, Fabio H., Langoni, Hélio [UNESP], Richini-Pereira, Virginia B., Lucheis, Simone Baldini [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/1678-9199-20-20
http://hdl.handle.net/11449/112347
Resumo: Background: With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, Sao Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results: MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative.Conclusions: PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis.
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spelling Leptospira spp. infection in sheep herds in southeast BrazilLeptospirosisOvineSerologyCulturePCRBackground: With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, Sao Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results: MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative.Conclusions: PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Regional Laboratory of Sorocaba - Adolfo Lutz InstituteUniv Estadual Paulista, Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Husb,Dept Vet Hyg & Publ Hlth, Botucatu, SP, BrazilAdolfo Lutz Inst, Sorocaba, SP, BrazilAdolfo Lutz Inst CLR II, Bauru, SP, BrazilPaulista Agcy Agribusiness Technol APTA SAA, BR-17030000 Bauru, SP, BrazilUniv Estadual Paulista, Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Husb,Dept Vet Hyg & Publ Hlth, Botucatu, SP, BrazilFAPESP: 08/52537-0FAPESP: 08/53714-2Biomed Central Ltd.Universidade Estadual Paulista (Unesp)Instituto Adolfo Lutz (IAL)Universidade de São Paulo (USP)Barbante, Priscila [UNESP]Shimabukuro, Fabio H.Langoni, Hélio [UNESP]Richini-Pereira, Virginia B.Lucheis, Simone Baldini [UNESP]2014-12-03T13:10:38Z2014-12-03T13:10:38Z2014-05-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1186/1678-9199-20-20Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Biomed Central Ltd, v. 20, 7 p., 2014.1678-9199http://hdl.handle.net/11449/11234710.1186/1678-9199-20-20WOS:000335792300002WOS000335792300002.pdf5326072118518067Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-12-25T06:19:31Zoai:repositorio.unesp.br:11449/112347Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:14:36.875956Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Leptospira spp. infection in sheep herds in southeast Brazil
title Leptospira spp. infection in sheep herds in southeast Brazil
spellingShingle Leptospira spp. infection in sheep herds in southeast Brazil
Barbante, Priscila [UNESP]
Leptospirosis
Ovine
Serology
Culture
PCR
title_short Leptospira spp. infection in sheep herds in southeast Brazil
title_full Leptospira spp. infection in sheep herds in southeast Brazil
title_fullStr Leptospira spp. infection in sheep herds in southeast Brazil
title_full_unstemmed Leptospira spp. infection in sheep herds in southeast Brazil
title_sort Leptospira spp. infection in sheep herds in southeast Brazil
author Barbante, Priscila [UNESP]
author_facet Barbante, Priscila [UNESP]
Shimabukuro, Fabio H.
Langoni, Hélio [UNESP]
Richini-Pereira, Virginia B.
Lucheis, Simone Baldini [UNESP]
author_role author
author2 Shimabukuro, Fabio H.
Langoni, Hélio [UNESP]
Richini-Pereira, Virginia B.
Lucheis, Simone Baldini [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto Adolfo Lutz (IAL)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Barbante, Priscila [UNESP]
Shimabukuro, Fabio H.
Langoni, Hélio [UNESP]
Richini-Pereira, Virginia B.
Lucheis, Simone Baldini [UNESP]
dc.subject.por.fl_str_mv Leptospirosis
Ovine
Serology
Culture
PCR
topic Leptospirosis
Ovine
Serology
Culture
PCR
description Background: With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, Sao Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results: MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative.Conclusions: PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-03T13:10:38Z
2014-12-03T13:10:38Z
2014-05-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1678-9199-20-20
Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Biomed Central Ltd, v. 20, 7 p., 2014.
1678-9199
http://hdl.handle.net/11449/112347
10.1186/1678-9199-20-20
WOS:000335792300002
WOS000335792300002.pdf
5326072118518067
url http://dx.doi.org/10.1186/1678-9199-20-20
http://hdl.handle.net/11449/112347
identifier_str_mv Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Biomed Central Ltd, v. 20, 7 p., 2014.
1678-9199
10.1186/1678-9199-20-20
WOS:000335792300002
WOS000335792300002.pdf
5326072118518067
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
1.782
0,573
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd.
publisher.none.fl_str_mv Biomed Central Ltd.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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