Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bios.2016.07.096 http://hdl.handle.net/11449/173750 |
Resumo: | Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets. |
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Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomoleculesbiotinylated biomoleculesimmunomagnetic separationmagnetic molecularly-imprinted polymermagnetic particlesmagneto-actuated immunoassayMagnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Ministerio de Economía y CompetitividadGrup de Sensors i Biosensors Departament de Química Universitat Autònoma de BarcelonaUNESP – Univ Estadual Paulista Institute of Chemistry Department of Analytical ChemistryUNESP – Univ Estadual Paulista Institute of Chemistry Department of Analytical ChemistryCNPq: 151525/2013-7CNPq: 303979/2012-7CNPq: 4004759/2012-4Ministerio de Economía y Competitividad: BIO2013-41242-RUniversitat Autònoma de BarcelonaUniversidade Estadual Paulista (Unesp)Ben Aissa, A.Herrera-Chacon, A.Pupin, R. R. [UNESP]Sotomayor, M. D.P.T. [UNESP]Pividori, M. I.2018-12-11T17:07:34Z2018-12-11T17:07:34Z2017-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article101-108application/pdfhttp://dx.doi.org/10.1016/j.bios.2016.07.096Biosensors and Bioelectronics, v. 88, p. 101-108.1873-42350956-5663http://hdl.handle.net/11449/17375010.1016/j.bios.2016.07.0962-s2.0-849945835022-s2.0-84994583502.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiosensors and Bioelectronics2,373info:eu-repo/semantics/openAccess2023-12-05T06:19:35Zoai:repositorio.unesp.br:11449/173750Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:34:03.223241Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules |
title |
Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules |
spellingShingle |
Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules Ben Aissa, A. biotinylated biomolecules immunomagnetic separation magnetic molecularly-imprinted polymer magnetic particles magneto-actuated immunoassay |
title_short |
Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules |
title_full |
Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules |
title_fullStr |
Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules |
title_full_unstemmed |
Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules |
title_sort |
Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules |
author |
Ben Aissa, A. |
author_facet |
Ben Aissa, A. Herrera-Chacon, A. Pupin, R. R. [UNESP] Sotomayor, M. D.P.T. [UNESP] Pividori, M. I. |
author_role |
author |
author2 |
Herrera-Chacon, A. Pupin, R. R. [UNESP] Sotomayor, M. D.P.T. [UNESP] Pividori, M. I. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ben Aissa, A. Herrera-Chacon, A. Pupin, R. R. [UNESP] Sotomayor, M. D.P.T. [UNESP] Pividori, M. I. |
dc.subject.por.fl_str_mv |
biotinylated biomolecules immunomagnetic separation magnetic molecularly-imprinted polymer magnetic particles magneto-actuated immunoassay |
topic |
biotinylated biomolecules immunomagnetic separation magnetic molecularly-imprinted polymer magnetic particles magneto-actuated immunoassay |
description |
Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-15 2018-12-11T17:07:34Z 2018-12-11T17:07:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bios.2016.07.096 Biosensors and Bioelectronics, v. 88, p. 101-108. 1873-4235 0956-5663 http://hdl.handle.net/11449/173750 10.1016/j.bios.2016.07.096 2-s2.0-84994583502 2-s2.0-84994583502.pdf |
url |
http://dx.doi.org/10.1016/j.bios.2016.07.096 http://hdl.handle.net/11449/173750 |
identifier_str_mv |
Biosensors and Bioelectronics, v. 88, p. 101-108. 1873-4235 0956-5663 10.1016/j.bios.2016.07.096 2-s2.0-84994583502 2-s2.0-84994583502.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biosensors and Bioelectronics 2,373 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
101-108 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129087018893312 |