Development and characterization of cefoxitin loaded D,L-PLA nanoparticles

Detalhes bibliográficos
Autor(a) principal: Machado, Silvia Regina Pengo
Data de Publicação: 2010
Outros Autores: Evangelista, Raul Cesar [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/977
http://hdl.handle.net/11449/133735
Resumo: Nanoparticles containing cefoxitin (CEF) made of D,L-PLA (PLA) were designed by a multiple emulsion/solvent evaporation method. The particles were extensively evaluated in relation to morphology, encapsulation efficiency, drug-polymer interactions as well as in vitro drug release kinetics. Nanoparticles were spherical in shape and isolated, with a mean diameter of about 600 nm. The thermal behaviour (DSC) of CEF-containing nanoparticles suggested that the drug was dispersed at a molecular level within the system. The drug encapsulation efficiency in the system for a CEF concentration of 30 mg/mL was 5.5%, as assessed after the drug extraction, by a validated HPLC method. This low encapsulation efficiency is understandable, since CEF is highly hydrophilic. The in vitro assays showed a strong sustained drug release profile from the nanoparticles with kinetics following pure Fickian diffusion.
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spelling Development and characterization of cefoxitin loaded D,L-PLA nanoparticlesDesenvolvimento e caracterização de nanopartículas de D,L-PLA contendo cefoxitinaNanoparticlesControlled releaseD,L-PLACefoxitinIn vitro releaseNanopartículasLiberação controladaD,L-PLACefoxitinaLiberação In vitroNanoparticles containing cefoxitin (CEF) made of D,L-PLA (PLA) were designed by a multiple emulsion/solvent evaporation method. The particles were extensively evaluated in relation to morphology, encapsulation efficiency, drug-polymer interactions as well as in vitro drug release kinetics. Nanoparticles were spherical in shape and isolated, with a mean diameter of about 600 nm. The thermal behaviour (DSC) of CEF-containing nanoparticles suggested that the drug was dispersed at a molecular level within the system. The drug encapsulation efficiency in the system for a CEF concentration of 30 mg/mL was 5.5%, as assessed after the drug extraction, by a validated HPLC method. This low encapsulation efficiency is understandable, since CEF is highly hydrophilic. The in vitro assays showed a strong sustained drug release profile from the nanoparticles with kinetics following pure Fickian diffusion.As nanopartículas de D, L-PLA (PLA) contendo cefoxitina (CEF) foram preparadas pelo método de emulsão múltipla / evaporação do solvente. As partículas foram avaliadas em relação à morfologia, à eficiência de encapsulação, às interações polímero-fármaco, bem como à cinética de liberação do fármaco in vitro . As nanopartículas são esféricas e isoladas, com um diâmetro médio de cerca de 600 nm. O comportamento térmico (DSC) das nanopartículas contendo CEF sugeriu que o fármaco está disperso em um nível molecular dentro do sistema. A eficiência de encapsulação do fármaco no sistema quando a concentração de CEF é 30 mg / mL foi de 5,5%, determinada após a extração de fármaco, através de um método de HPLC validado. Esta baixa eficiência de encapsulação é compreensível, uma vez que a CEF é altamente hidrofílica. Os ensaios in vitro mostraram um perfil de liberação do fármaco a partir das nanopartículas fortemente sustentado e com uma cinética de difusão Fickiana pura.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista, Departamento de Fármacos e Medicamentos, Faculdade de Ciências Farmacêuticas de AraraquaraUniversidade Metodista de Piracicaba (UNIMEP)Universidade Estadual Paulista (Unesp)Machado, Silvia Regina PengoEvangelista, Raul Cesar [UNESP]2016-01-28T16:56:23Z2016-01-28T16:56:23Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article193-202application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/977Revista de Ciências Farmacêuticas Básica e Aplicada, v. 31, n. 3, p. 193-202, 2010.1808-4532http://hdl.handle.net/11449/133735ISSN1808-4532-2010-31-03-193-202.pdf40522082516073185361569184579557Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2024-06-24T13:46:24Zoai:repositorio.unesp.br:11449/133735Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:42:51.589701Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development and characterization of cefoxitin loaded D,L-PLA nanoparticles
Desenvolvimento e caracterização de nanopartículas de D,L-PLA contendo cefoxitina
title Development and characterization of cefoxitin loaded D,L-PLA nanoparticles
spellingShingle Development and characterization of cefoxitin loaded D,L-PLA nanoparticles
Machado, Silvia Regina Pengo
Nanoparticles
Controlled release
D,L-PLA
Cefoxitin
In vitro release
Nanopartículas
Liberação controlada
D,L-PLA
Cefoxitina
Liberação In vitro
title_short Development and characterization of cefoxitin loaded D,L-PLA nanoparticles
title_full Development and characterization of cefoxitin loaded D,L-PLA nanoparticles
title_fullStr Development and characterization of cefoxitin loaded D,L-PLA nanoparticles
title_full_unstemmed Development and characterization of cefoxitin loaded D,L-PLA nanoparticles
title_sort Development and characterization of cefoxitin loaded D,L-PLA nanoparticles
author Machado, Silvia Regina Pengo
author_facet Machado, Silvia Regina Pengo
Evangelista, Raul Cesar [UNESP]
author_role author
author2 Evangelista, Raul Cesar [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Metodista de Piracicaba (UNIMEP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Machado, Silvia Regina Pengo
Evangelista, Raul Cesar [UNESP]
dc.subject.por.fl_str_mv Nanoparticles
Controlled release
D,L-PLA
Cefoxitin
In vitro release
Nanopartículas
Liberação controlada
D,L-PLA
Cefoxitina
Liberação In vitro
topic Nanoparticles
Controlled release
D,L-PLA
Cefoxitin
In vitro release
Nanopartículas
Liberação controlada
D,L-PLA
Cefoxitina
Liberação In vitro
description Nanoparticles containing cefoxitin (CEF) made of D,L-PLA (PLA) were designed by a multiple emulsion/solvent evaporation method. The particles were extensively evaluated in relation to morphology, encapsulation efficiency, drug-polymer interactions as well as in vitro drug release kinetics. Nanoparticles were spherical in shape and isolated, with a mean diameter of about 600 nm. The thermal behaviour (DSC) of CEF-containing nanoparticles suggested that the drug was dispersed at a molecular level within the system. The drug encapsulation efficiency in the system for a CEF concentration of 30 mg/mL was 5.5%, as assessed after the drug extraction, by a validated HPLC method. This low encapsulation efficiency is understandable, since CEF is highly hydrophilic. The in vitro assays showed a strong sustained drug release profile from the nanoparticles with kinetics following pure Fickian diffusion.
publishDate 2010
dc.date.none.fl_str_mv 2010
2016-01-28T16:56:23Z
2016-01-28T16:56:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/977
Revista de Ciências Farmacêuticas Básica e Aplicada, v. 31, n. 3, p. 193-202, 2010.
1808-4532
http://hdl.handle.net/11449/133735
ISSN1808-4532-2010-31-03-193-202.pdf
4052208251607318
5361569184579557
url http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/977
http://hdl.handle.net/11449/133735
identifier_str_mv Revista de Ciências Farmacêuticas Básica e Aplicada, v. 31, n. 3, p. 193-202, 2010.
1808-4532
ISSN1808-4532-2010-31-03-193-202.pdf
4052208251607318
5361569184579557
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista de Ciências Farmacêuticas Básica e Aplicada
0,131
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 193-202
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129453330530304

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