Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)

Detalhes bibliográficos
Autor(a) principal: Zampieri, Bruna Del Busso [UNESP]
Data de Publicação: 2022
Outros Autores: Nogueira, Elis Watanabe, de Oliveira, Ana Julia Fernandes Cardoso [UNESP], Sánchez-Andrea, Irene, Brucha, Gunther
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s11356-021-15418-9
http://hdl.handle.net/11449/229266
Resumo: Sulfate-reducing bacteria (SRB) can be used to remove metals from wastewater, sewage, and contaminated areas. However, metals can be toxic to this group of bacteria. Sediments from port areas present abundance of SRB and also metal contamination. Their microbial community has been exposed to metals and can be a good inoculum for isolation of metal-resistant SRB. The objective of the study was to analyze how metals influence activity and composition of sulfate-reducing bacteria. Enrichment cultures were prepared with a different metal (Zn, Cr, Cu, and Cd) range concentration tracking activity of SRB and 16S rRNA sequencing in order to access the community. The SRB activity decreased when there was an increase in the concentration of the metals tested. The highest concentration of metals precipitated were 0.2 mM of Cd, 5.4 mM of Zn, 4.5 mM of Cu, and 9.6 mM of Cr. The more toxic metals were Cd and Cu and had a greater community similarity with less SRB and more fermenters (e.g., Citrobacter and Clostridium). Meanwhile, the enrichments with less toxic metals (Cr and Zn) had more sequences affiliated to SRB genera (mainly Desulfovibrio). A new Desulfovibrio species was isolated. This type of study can be useful to understand the effects of metals in SRB communities and help to optimize wastewater treatment processes contaminated by metals. The new Desulfovibrio species may be important in future studies on bioremediation of neutral pH effluents contaminated by metals.
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spelling Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)BioremediationDesulfovibrioMetal removalMetal resistanceMetalsSulfate-reducing bacteriaSulfate-reducing bacteria (SRB) can be used to remove metals from wastewater, sewage, and contaminated areas. However, metals can be toxic to this group of bacteria. Sediments from port areas present abundance of SRB and also metal contamination. Their microbial community has been exposed to metals and can be a good inoculum for isolation of metal-resistant SRB. The objective of the study was to analyze how metals influence activity and composition of sulfate-reducing bacteria. Enrichment cultures were prepared with a different metal (Zn, Cr, Cu, and Cd) range concentration tracking activity of SRB and 16S rRNA sequencing in order to access the community. The SRB activity decreased when there was an increase in the concentration of the metals tested. The highest concentration of metals precipitated were 0.2 mM of Cd, 5.4 mM of Zn, 4.5 mM of Cu, and 9.6 mM of Cr. The more toxic metals were Cd and Cu and had a greater community similarity with less SRB and more fermenters (e.g., Citrobacter and Clostridium). Meanwhile, the enrichments with less toxic metals (Cr and Zn) had more sequences affiliated to SRB genera (mainly Desulfovibrio). A new Desulfovibrio species was isolated. This type of study can be useful to understand the effects of metals in SRB communities and help to optimize wastewater treatment processes contaminated by metals. The new Desulfovibrio species may be important in future studies on bioremediation of neutral pH effluents contaminated by metals.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Biochemistry and Microbiology Biosciences Institute São Paulo State University – Rio Claro Campus (UNESP IB/RC)), Av. 24 A, 1515, Jardim Vila Bela, Rio ClaroBiological Processes Laboratory São Carlos School of Engineering University of São Paulo, Av. João Dagnone, 1100, Santa Angelina, São CarlosBiosciences Institute São Paulo State University – São Paulo State´s Coast Campus (UNESP IB/CLP), Praça Infante Dom Henrique, s/n - Parque BitaruDepartment of Agrotechnology and Food Sciences Wageningen University and Research, Stippeneng 4, 6708WESchool of Technological Sciences Federal University of Alfenas (UNIFAL-MG) Minas Gerais, Rodovia Aurélio Vilela, n 11.999 Cidade Universitária, Minas GeraisDepartment of Biochemistry and Microbiology Biosciences Institute São Paulo State University – Rio Claro Campus (UNESP IB/RC)), Av. 24 A, 1515, Jardim Vila Bela, Rio ClaroBiosciences Institute São Paulo State University – São Paulo State´s Coast Campus (UNESP IB/CLP), Praça Infante Dom Henrique, s/n - Parque BitaruCAPES: 88882.434109/2019-01Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Wageningen University and ResearchMinas GeraisZampieri, Bruna Del Busso [UNESP]Nogueira, Elis Watanabede Oliveira, Ana Julia Fernandes Cardoso [UNESP]Sánchez-Andrea, IreneBrucha, Gunther2022-04-29T08:31:29Z2022-04-29T08:31:29Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article922-935http://dx.doi.org/10.1007/s11356-021-15418-9Environmental Science and Pollution Research, v. 29, n. 1, p. 922-935, 2022.1614-74990944-1344http://hdl.handle.net/11449/22926610.1007/s11356-021-15418-92-s2.0-85111844219Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Researchinfo:eu-repo/semantics/openAccess2022-04-29T08:31:29Zoai:repositorio.unesp.br:11449/229266Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:24:14.358334Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)
title Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)
spellingShingle Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)
Zampieri, Bruna Del Busso [UNESP]
Bioremediation
Desulfovibrio
Metal removal
Metal resistance
Metals
Sulfate-reducing bacteria
title_short Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)
title_full Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)
title_fullStr Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)
title_full_unstemmed Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)
title_sort Effects of metals on activity and community of sulfate-reducing bacterial enrichments and the discovery of a new heavy metal-resistant SRB from Santos Port sediment (São Paulo, Brazil)
author Zampieri, Bruna Del Busso [UNESP]
author_facet Zampieri, Bruna Del Busso [UNESP]
Nogueira, Elis Watanabe
de Oliveira, Ana Julia Fernandes Cardoso [UNESP]
Sánchez-Andrea, Irene
Brucha, Gunther
author_role author
author2 Nogueira, Elis Watanabe
de Oliveira, Ana Julia Fernandes Cardoso [UNESP]
Sánchez-Andrea, Irene
Brucha, Gunther
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
Wageningen University and Research
Minas Gerais
dc.contributor.author.fl_str_mv Zampieri, Bruna Del Busso [UNESP]
Nogueira, Elis Watanabe
de Oliveira, Ana Julia Fernandes Cardoso [UNESP]
Sánchez-Andrea, Irene
Brucha, Gunther
dc.subject.por.fl_str_mv Bioremediation
Desulfovibrio
Metal removal
Metal resistance
Metals
Sulfate-reducing bacteria
topic Bioremediation
Desulfovibrio
Metal removal
Metal resistance
Metals
Sulfate-reducing bacteria
description Sulfate-reducing bacteria (SRB) can be used to remove metals from wastewater, sewage, and contaminated areas. However, metals can be toxic to this group of bacteria. Sediments from port areas present abundance of SRB and also metal contamination. Their microbial community has been exposed to metals and can be a good inoculum for isolation of metal-resistant SRB. The objective of the study was to analyze how metals influence activity and composition of sulfate-reducing bacteria. Enrichment cultures were prepared with a different metal (Zn, Cr, Cu, and Cd) range concentration tracking activity of SRB and 16S rRNA sequencing in order to access the community. The SRB activity decreased when there was an increase in the concentration of the metals tested. The highest concentration of metals precipitated were 0.2 mM of Cd, 5.4 mM of Zn, 4.5 mM of Cu, and 9.6 mM of Cr. The more toxic metals were Cd and Cu and had a greater community similarity with less SRB and more fermenters (e.g., Citrobacter and Clostridium). Meanwhile, the enrichments with less toxic metals (Cr and Zn) had more sequences affiliated to SRB genera (mainly Desulfovibrio). A new Desulfovibrio species was isolated. This type of study can be useful to understand the effects of metals in SRB communities and help to optimize wastewater treatment processes contaminated by metals. The new Desulfovibrio species may be important in future studies on bioremediation of neutral pH effluents contaminated by metals.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:31:29Z
2022-04-29T08:31:29Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11356-021-15418-9
Environmental Science and Pollution Research, v. 29, n. 1, p. 922-935, 2022.
1614-7499
0944-1344
http://hdl.handle.net/11449/229266
10.1007/s11356-021-15418-9
2-s2.0-85111844219
url http://dx.doi.org/10.1007/s11356-021-15418-9
http://hdl.handle.net/11449/229266
identifier_str_mv Environmental Science and Pollution Research, v. 29, n. 1, p. 922-935, 2022.
1614-7499
0944-1344
10.1007/s11356-021-15418-9
2-s2.0-85111844219
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Environmental Science and Pollution Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 922-935
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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