Detecção, clonagem e análise in silico do gene parasporina 4 em linhagens de Bacillus thuringiensis

• Autor(a) principal: Santos, Thais Nayara Ferreira dos
• Data de Publicação: 2021
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/214266
• topic: Bacillus thuringiensis; Parasporina; Câncer; Citotoxicidade; Genética molecular

Bacillus thuringiensis (Bt) is a gram-positive bacterium that has its strains studied mainly for the control of insect pests, due to the insecticidal capacity of most of its proteins (Cry and Vip). However, recent studies indicate the presence of other proteins with little known insecticidal action, but of great importance in research involving this microorganism. These proteins called Parasporins (PS) have cytotoxic activity and have six subfamilies, being PS1, PS2, PS3, PS4, PS5 and PS6, considered potential drugs in the treatment of cancer. Given the high incidence of cancer cases in the world and the need for new treatment alternatives, this study aimed to characterize B. thuringiensis strains, belonging to the collection of the Laboratório de Genética de Bactérias e Biotecnologia Aplicada at FCAV - UNESP, as potential sources of the PS4 protein, being the least studied as a subfamily. The selection of strains was performed using the PCR (Polymerase Chain Reaction) technique with specific primers for the ps4 gene, followed by cloning and sequencing of the amplicons of the positive responses, in order to establish a sequence analysis in silico. compared to existing locations in the GenBank database. The present study appears to be the selection of a strain carrying the ps4 gene, called B. thuringiensis coreanensis, whose partial sequence of the same was carried out for identity analysis with the database sequences and the observation that the ps4 gene is located on a plasmid. Therefore, this strain can be a source of the gene for future expression experiments and characterization of the cytotoxic effects of Parasporin 4 on different types of tumors.