Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0001-3765202120201583 http://hdl.handle.net/11449/230166 |
Resumo: | Animals corpses in teaching and research institutions could be sources of infection for students and teachers when applied for dissection and surgical practice. This research aimed to evaluate cats’ corpses’ conservation using a new anatomic technique and vacuum package for seven days, aiming surgical practicing. A 150 mL/ kg of alcohol with 5% glycerin and 120 mL/kg of a 20% sodium chloride, 1% nitrite, and 1% sodium nitrate solution was injected on corpses sealed in vacuum packages and put on 0 to 4°C. Skin and jejunum were collected on day 0 (fresh samples/control), and traction analysis was performed for seven consecutive days. On the last day, the liquid in the plastic bags was microbiologically analyzed. There was no statistical difference between control and conservation moments (D1 and D2) in maximal rupture force of the skin, and jejunum was similar to control in D2, D4, and D6. The microbial population did not exceed 6.0x104CFU/mL in total aerobics and 4.8x104CFU/mL in total anaerobes. Biomechanics was not significantly affected, and the microbiological count was low during conservation, demonstrating the possible effectiveness of this anatomical technique for surgery training. |
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Biomechanical and microbiological analysis of embalmed cats – acute effect of conservationAcute effectAnatomyCuring saltMicroorganismsSurgeryAnimals corpses in teaching and research institutions could be sources of infection for students and teachers when applied for dissection and surgical practice. This research aimed to evaluate cats’ corpses’ conservation using a new anatomic technique and vacuum package for seven days, aiming surgical practicing. A 150 mL/ kg of alcohol with 5% glycerin and 120 mL/kg of a 20% sodium chloride, 1% nitrite, and 1% sodium nitrate solution was injected on corpses sealed in vacuum packages and put on 0 to 4°C. Skin and jejunum were collected on day 0 (fresh samples/control), and traction analysis was performed for seven consecutive days. On the last day, the liquid in the plastic bags was microbiologically analyzed. There was no statistical difference between control and conservation moments (D1 and D2) in maximal rupture force of the skin, and jejunum was similar to control in D2, D4, and D6. The microbial population did not exceed 6.0x104CFU/mL in total aerobics and 4.8x104CFU/mL in total anaerobes. Biomechanics was not significantly affected, and the microbiological count was low during conservation, demonstrating the possible effectiveness of this anatomical technique for surgery training.Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Fisiologia e Morfologia Animal, Via de Acesso Paulo Donato Castelane, s/n, Campus Jaboticabal SPUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia Veterinária, Via de Acesso Paulo Donato Castelane, s/n, Campus Jaboticabal SPUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Fisiologia e Morfologia Animal, Via de Acesso Paulo Donato Castelane, s/n, Campus Jaboticabal SPUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Patologia Veterinária, Via de Acesso Paulo Donato Castelane, s/n, Campus Jaboticabal SPUniversidade Estadual Paulista (UNESP)Queiroz, Andréa B. P. S. [UNESP]Rodrigues, Alessandra [UNESP]Cardozo, Marita V. [UNESP]Costa, Natália T. B. [UNESP]Soares, Laura G. [UNESP]Fechis, Alisson D. S. [UNESP]Oliveira, Fabrício S. [UNESP]2022-04-29T08:38:13Z2022-04-29T08:38:13Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/0001-3765202120201583Anais da Academia Brasileira de Ciencias, v. 94, n. 1, 2022.1678-26900001-3765http://hdl.handle.net/11449/23016610.1590/0001-37652021202015832-s2.0-85122270356Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais da Academia Brasileira de Cienciasinfo:eu-repo/semantics/openAccess2024-06-07T13:02:27Zoai:repositorio.unesp.br:11449/230166Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:14:59.055918Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation |
title |
Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation |
spellingShingle |
Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation Queiroz, Andréa B. P. S. [UNESP] Acute effect Anatomy Curing salt Microorganisms Surgery |
title_short |
Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation |
title_full |
Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation |
title_fullStr |
Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation |
title_full_unstemmed |
Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation |
title_sort |
Biomechanical and microbiological analysis of embalmed cats – acute effect of conservation |
author |
Queiroz, Andréa B. P. S. [UNESP] |
author_facet |
Queiroz, Andréa B. P. S. [UNESP] Rodrigues, Alessandra [UNESP] Cardozo, Marita V. [UNESP] Costa, Natália T. B. [UNESP] Soares, Laura G. [UNESP] Fechis, Alisson D. S. [UNESP] Oliveira, Fabrício S. [UNESP] |
author_role |
author |
author2 |
Rodrigues, Alessandra [UNESP] Cardozo, Marita V. [UNESP] Costa, Natália T. B. [UNESP] Soares, Laura G. [UNESP] Fechis, Alisson D. S. [UNESP] Oliveira, Fabrício S. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Queiroz, Andréa B. P. S. [UNESP] Rodrigues, Alessandra [UNESP] Cardozo, Marita V. [UNESP] Costa, Natália T. B. [UNESP] Soares, Laura G. [UNESP] Fechis, Alisson D. S. [UNESP] Oliveira, Fabrício S. [UNESP] |
dc.subject.por.fl_str_mv |
Acute effect Anatomy Curing salt Microorganisms Surgery |
topic |
Acute effect Anatomy Curing salt Microorganisms Surgery |
description |
Animals corpses in teaching and research institutions could be sources of infection for students and teachers when applied for dissection and surgical practice. This research aimed to evaluate cats’ corpses’ conservation using a new anatomic technique and vacuum package for seven days, aiming surgical practicing. A 150 mL/ kg of alcohol with 5% glycerin and 120 mL/kg of a 20% sodium chloride, 1% nitrite, and 1% sodium nitrate solution was injected on corpses sealed in vacuum packages and put on 0 to 4°C. Skin and jejunum were collected on day 0 (fresh samples/control), and traction analysis was performed for seven consecutive days. On the last day, the liquid in the plastic bags was microbiologically analyzed. There was no statistical difference between control and conservation moments (D1 and D2) in maximal rupture force of the skin, and jejunum was similar to control in D2, D4, and D6. The microbial population did not exceed 6.0x104CFU/mL in total aerobics and 4.8x104CFU/mL in total anaerobes. Biomechanics was not significantly affected, and the microbiological count was low during conservation, demonstrating the possible effectiveness of this anatomical technique for surgery training. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:38:13Z 2022-04-29T08:38:13Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0001-3765202120201583 Anais da Academia Brasileira de Ciencias, v. 94, n. 1, 2022. 1678-2690 0001-3765 http://hdl.handle.net/11449/230166 10.1590/0001-3765202120201583 2-s2.0-85122270356 |
url |
http://dx.doi.org/10.1590/0001-3765202120201583 http://hdl.handle.net/11449/230166 |
identifier_str_mv |
Anais da Academia Brasileira de Ciencias, v. 94, n. 1, 2022. 1678-2690 0001-3765 10.1590/0001-3765202120201583 2-s2.0-85122270356 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anais da Academia Brasileira de Ciencias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129042145083392 |