Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00284-018-1459-3 http://hdl.handle.net/11449/175908 |
Resumo: | Camponotus is a hyper-diverse ant genus that is associated with the obligate endosymbiont Blochmannia, and often also with Wolbachia, but morphological studies on the location of these bacteria in the queen’s ovaries during oogenesis remain limited. In the present study, we used the Neotropical weaver ant Camponotus textor to characterize the ovary using histology (HE) techniques, and to document the location of Blochmannia and Wolbachia during oogenesis through fluorescence in situ hybridization (FISH). This is the first morphological report of these two bacteria in the same host with polytrophic meroistic ovaries and reveals that Blochmannia is found inside late-stage oocytes and Wolbachia is associated with the nuclei of the nurse cells. Our results provide insights into the developmental sequence of when these bacteria reach the egg, with Blochmannia establishing itself in the egg first, and Wolbachia only reaching the egg shortly before completing egg development. Studies such as this provide understanding about the mechanisms and timing of the establishment of these endosymbionts in the host. |
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spelling |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae)Camponotus is a hyper-diverse ant genus that is associated with the obligate endosymbiont Blochmannia, and often also with Wolbachia, but morphological studies on the location of these bacteria in the queen’s ovaries during oogenesis remain limited. In the present study, we used the Neotropical weaver ant Camponotus textor to characterize the ovary using histology (HE) techniques, and to document the location of Blochmannia and Wolbachia during oogenesis through fluorescence in situ hybridization (FISH). This is the first morphological report of these two bacteria in the same host with polytrophic meroistic ovaries and reveals that Blochmannia is found inside late-stage oocytes and Wolbachia is associated with the nuclei of the nurse cells. Our results provide insights into the developmental sequence of when these bacteria reach the egg, with Blochmannia establishing itself in the egg first, and Wolbachia only reaching the egg shortly before completing egg development. Studies such as this provide understanding about the mechanisms and timing of the establishment of these endosymbionts in the host.Barcapel FoundationConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departament of Biology e Center for Studies on Social Insects Biosciense Institute São Paulo State University (UNESP), Campus Rio Claro, Avenida 24A, 1515, Bela VistaField Museum of Natural History Department of Science and Education Integrative Research Center, 1400 South Lake Shore DriveDepartament of Biology e Center for Studies on Social Insects Biosciense Institute São Paulo State University (UNESP), Campus Rio Claro, Avenida 24A, 1515, Bela VistaBarcapel Foundation: 007343/2014-00CNPq: 157837/2015-7Universidade Estadual Paulista (Unesp)Integrative Research CenterRamalho, Manuela Oliveira [UNESP]Vieira, Alexsandro Santana [UNESP]Pereira, Mayara Cristina [UNESP]Moreau, Corrie SauxBueno, Odair Correa [UNESP]2018-12-11T17:18:05Z2018-12-11T17:18:05Z2018-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article866-873application/pdfhttp://dx.doi.org/10.1007/s00284-018-1459-3Current Microbiology, v. 75, n. 7, p. 866-873, 2018.1432-09910343-8651http://hdl.handle.net/11449/17590810.1007/s00284-018-1459-32-s2.0-850422228962-s2.0-85042222896.pdf10507090557764280000-0002-3586-6192Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Microbiology0,5620,562info:eu-repo/semantics/openAccess2023-11-03T06:13:44Zoai:repositorio.unesp.br:11449/175908Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-03T06:13:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae) |
title |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae) |
spellingShingle |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae) Ramalho, Manuela Oliveira [UNESP] |
title_short |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae) |
title_full |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae) |
title_fullStr |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae) |
title_full_unstemmed |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae) |
title_sort |
Transovarian Transmission of Blochmannia and Wolbachia Endosymbionts in the Neotropical Weaver Ant Camponotus textor (Hymenoptera, Formicidae) |
author |
Ramalho, Manuela Oliveira [UNESP] |
author_facet |
Ramalho, Manuela Oliveira [UNESP] Vieira, Alexsandro Santana [UNESP] Pereira, Mayara Cristina [UNESP] Moreau, Corrie Saux Bueno, Odair Correa [UNESP] |
author_role |
author |
author2 |
Vieira, Alexsandro Santana [UNESP] Pereira, Mayara Cristina [UNESP] Moreau, Corrie Saux Bueno, Odair Correa [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Integrative Research Center |
dc.contributor.author.fl_str_mv |
Ramalho, Manuela Oliveira [UNESP] Vieira, Alexsandro Santana [UNESP] Pereira, Mayara Cristina [UNESP] Moreau, Corrie Saux Bueno, Odair Correa [UNESP] |
description |
Camponotus is a hyper-diverse ant genus that is associated with the obligate endosymbiont Blochmannia, and often also with Wolbachia, but morphological studies on the location of these bacteria in the queen’s ovaries during oogenesis remain limited. In the present study, we used the Neotropical weaver ant Camponotus textor to characterize the ovary using histology (HE) techniques, and to document the location of Blochmannia and Wolbachia during oogenesis through fluorescence in situ hybridization (FISH). This is the first morphological report of these two bacteria in the same host with polytrophic meroistic ovaries and reveals that Blochmannia is found inside late-stage oocytes and Wolbachia is associated with the nuclei of the nurse cells. Our results provide insights into the developmental sequence of when these bacteria reach the egg, with Blochmannia establishing itself in the egg first, and Wolbachia only reaching the egg shortly before completing egg development. Studies such as this provide understanding about the mechanisms and timing of the establishment of these endosymbionts in the host. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:05Z 2018-12-11T17:18:05Z 2018-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00284-018-1459-3 Current Microbiology, v. 75, n. 7, p. 866-873, 2018. 1432-0991 0343-8651 http://hdl.handle.net/11449/175908 10.1007/s00284-018-1459-3 2-s2.0-85042222896 2-s2.0-85042222896.pdf 1050709055776428 0000-0002-3586-6192 |
url |
http://dx.doi.org/10.1007/s00284-018-1459-3 http://hdl.handle.net/11449/175908 |
identifier_str_mv |
Current Microbiology, v. 75, n. 7, p. 866-873, 2018. 1432-0991 0343-8651 10.1007/s00284-018-1459-3 2-s2.0-85042222896 2-s2.0-85042222896.pdf 1050709055776428 0000-0002-3586-6192 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Current Microbiology 0,562 0,562 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
866-873 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964809167372288 |