Os efeitos da alta temperatura e do myleran (busulfan) na espermatogênese de Devario aequipinnatus (teleostei, cyprinidae)

• Autor(a) principal: Chagas, Jumma Miranda Araújo [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/126296; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/12-08-2015/000843900.pdf
• topic: Espermatogenese em animais; Celulas germinativas; Peixe - Pesquisa; Testiculos; Spermatogenesis in animals

In teleosts, the use of spermatogonial stem transplant is creating a new and promising scenario in the area of biotechnology and aquaculture production, enabling the preservation of species in danger of extinction. This technique involves the removal of spermatogonial stem cells of the testis of a donor animal and its transfer to the testicle of a receiver. To ensure the efficiency of this technique the receiver shall have its endogenous spermatogenesis deleted because the transplant efficiency depends on the success in the competition between transplanted germ cells and endogenous germ cells in the seminiferous tubules. This study aimed to experimentally promote depletion of endogenous spermatogenesis Devario aequipinnatus through the chemotherapy drug Myleran (Busulfan) and its association with the temperature 30 ° C. Maturation of four phases have been defined for testicular cycle D. aequipinnatus: Maturation Home, Intermediate maturation, maturation and Final Regression, these definitions were based on the changes of the testicular germinal epithelium associated with the stages of germ cells present. For depletion two doses were tested in the T15 / 18 were applied two injections via intracelomic with an interval of 10 days between them, the first dose of 15 mg kg-1 Myleran (Busulfan) and second with 18 mg kg- 1 Myleran. T40 was applied only in an injection containing 40 mg kg-1 Myleran (Busulfan). The control group was used an experimental unit without the inclusion of any type of drug, where the animals were kept under the same conditions of temperature, photoperiod and food offered to the animals used treatments. The T15 / 18 and T40 showed a significant reduction in the number of spermatocytes and consequently an increase in the amount tubular lumen in the testes of the animals examined. Three days after application of Busulfan for both single dose and double dose of Busulfan for an increase in pyknosis in spermatogonia ...