Anti-inflammatory intestinal activity of Combretum duarteanum Cambess. in trinitrobenzene sulfonic acid colitis model

• Autor(a) principal: Morais Lima, Gedson Rodrigues de
• Data de Publicação: 2017
• Outros Autores: Frota Machado, Flavia Danniele, Lucena Perico, Larissa [UNESP], Meira de Faria, Felipe, Luiz-Ferreira, Anderson, Souza Brito, Alba Regina Monteiro, Helena Pellizzon, Cludia [UNESP], Akiko Hiruma-Lima, Claudia [UNESP], Fechine Tavares, Josean, Barbosa Filho, Jose Maria, Maria Batista, Leonia
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.3748/wjg.v23.i8.1353; http://hdl.handle.net/11449/159419
• topic: Combretum duarteanum; Medicinal plants; Combretaceae; Inflammatory bowel disease; Anti-inflammatory intestinal activity

AIM To evaluate the anti-inflammatory intestinal effect of the ethanolic extract (EtOHE) and hexane phase (HexP) obtained from the leaves of Combretum duarteanum (Cd). METHODS Inflammatory bowel disease was induced using trinitrobenzenesulfonic acid in acute and relapsed ulcerative colitis in rat models. Damage scores, and biochemical, histological and immunohistochemical parameters were evaluated. RESULTS Both Cd-EtOHE and Cd-HexP caused significant reductions in macroscopic lesion scores and ulcerative lesion areas. The vegetable samples inhibited myeloperoxidase increase, as well as pro-inflammatory cytokines TNF-alpha and IL-1 beta. Anti-inflammatory cytokine IL-10 also increased in animals treated with the tested plant samples. The anti-inflammatory intestinal effect is related to decreased expression of cyclooxygenase-2, proliferating cell nuclear antigen, and an increase in superoxide dismutase. CONCLUSION The data indicate anti-inflammatory intestinal activity. The effects may also involve participation of the antioxidant system and principal cytokines relating to inflammatory bowel disease.