Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.foodres.2022.111955 http://hdl.handle.net/11449/247710 |
Resumo: | Foodborne infections caused by Salmonella have been linked to a variety of poultry products. The aim of this study was to compare the molecular profile of virulence genes considering different serotypes of Salmonella, isolates were from chicken breast sampled during the last two decades (1999 to 2010 and 2011 to 2018). The resistance to antimicrobials was also evaluated, establishing a comparative epidemiological parameter on the pathogenic potential on this bacterium over time. We tested 238 Salmonella isolates, and 18 different serotypes were observed. These being S. Enteritidis (42.3%, 58/137) and S. Ohio (28.3%, 36/137), the most frequent in the first decade; and S. Heidelberg (25.7%, 26/101) and S. Typhimurium (21.8%, 22/101), in the second. We found four (1.68%) multidrug resistant isolates from the first decade and 28 (11.76%) in the second. All extended spectrum beta-lactamase (ESBL) positive isolates belonged to the S. Heidelberg serotype, and were also detected in the second decade. Considering the nine different antimicrobial classes tested, an increase in the number of resistant isolates was observed over time: from five classes with resistant isolates in the first decade to eight classes in the second, with cefotaxime being the antimicrobial with the highest number of resistant isolates in both decades. All isolates (100%) presented the invA, sitC and tolC genes. In sequence, the most frequent genes were flgL (99.6%), sopB (98.3%), flgK (97.9%), fljB (96.6%), sipA (94.9%), sipB (88.6%), sifA (86.4%), sipD (66.1%), ssaR (51.3%), sopD (37.3%) and spvB (34.3%) was the least frequent; and 13 isolates showing all 14 virulence genes investigated. The ability of these isolates to resist certain antimicrobials, and to express genes encoding virulence factors, reinforce their marked pathogenic potential; while the possibility to trigger diseases in humans through the food chain is a serious public health threat through. |
id |
UNSP_7af1871988daabd2b353b6517cd277fb |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/247710 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in BrazilESBLFoodborne diseaseMultidrug resistancePublic healthS. HeidelbergVirulenceFoodborne infections caused by Salmonella have been linked to a variety of poultry products. The aim of this study was to compare the molecular profile of virulence genes considering different serotypes of Salmonella, isolates were from chicken breast sampled during the last two decades (1999 to 2010 and 2011 to 2018). The resistance to antimicrobials was also evaluated, establishing a comparative epidemiological parameter on the pathogenic potential on this bacterium over time. We tested 238 Salmonella isolates, and 18 different serotypes were observed. These being S. Enteritidis (42.3%, 58/137) and S. Ohio (28.3%, 36/137), the most frequent in the first decade; and S. Heidelberg (25.7%, 26/101) and S. Typhimurium (21.8%, 22/101), in the second. We found four (1.68%) multidrug resistant isolates from the first decade and 28 (11.76%) in the second. All extended spectrum beta-lactamase (ESBL) positive isolates belonged to the S. Heidelberg serotype, and were also detected in the second decade. Considering the nine different antimicrobial classes tested, an increase in the number of resistant isolates was observed over time: from five classes with resistant isolates in the first decade to eight classes in the second, with cefotaxime being the antimicrobial with the highest number of resistant isolates in both decades. All isolates (100%) presented the invA, sitC and tolC genes. In sequence, the most frequent genes were flgL (99.6%), sopB (98.3%), flgK (97.9%), fljB (96.6%), sipA (94.9%), sipB (88.6%), sifA (86.4%), sipD (66.1%), ssaR (51.3%), sopD (37.3%) and spvB (34.3%) was the least frequent; and 13 isolates showing all 14 virulence genes investigated. The ability of these isolates to resist certain antimicrobials, and to express genes encoding virulence factors, reinforce their marked pathogenic potential; while the possibility to trigger diseases in humans through the food chain is a serious public health threat through.Department of Food Sciences and Nutrition Faculty of Food Engineering State University of Campinas (UNICAMP)Sector of Microbiology and Immunology Department of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University (UNESP)Enteric Diseases and Infections by Special Pathogens Nucleus Bacteriology Center Adolfo Lutz InstituteDepartment of Animal Production and Preventive Veterinary Medicine São Paulo State UniversitySector of Microbiology and Immunology Department of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Animal Production and Preventive Veterinary Medicine São Paulo State UniversityUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Adolfo Lutz InstituteMorasi, Rafaela Martinsda Silva, André Zimbardi [UNESP]Nuñez, Karen Vanessa MuniveDantas, Stéfani Thais Alves [UNESP]Faganello, Caroline [UNESP]Juliano, Lara Cristina Bastos [UNESP]Tiba-Casas, Monique RibeiroPantoja, José C.F. [UNESP]Amarante, Ariadne FerreiraJúnior, Ary Fernandes [UNESP]Rall, Vera Lúcia Mores [UNESP]Silva, Nathália Cristina Cirone2023-07-29T13:23:44Z2023-07-29T13:23:44Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.foodres.2022.111955Food Research International, v. 162.1873-71450963-9969http://hdl.handle.net/11449/24771010.1016/j.foodres.2022.1119552-s2.0-85139291262Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Research Internationalinfo:eu-repo/semantics/openAccess2023-07-29T13:23:44Zoai:repositorio.unesp.br:11449/247710Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:47:00.980468Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil |
title |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil |
spellingShingle |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil Morasi, Rafaela Martins ESBL Foodborne disease Multidrug resistance Public health S. Heidelberg Virulence |
title_short |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil |
title_full |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil |
title_fullStr |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil |
title_full_unstemmed |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil |
title_sort |
Overview of antimicrobial resistance and virulence factors in Salmonella spp. isolated in the last two decades from chicken in Brazil |
author |
Morasi, Rafaela Martins |
author_facet |
Morasi, Rafaela Martins da Silva, André Zimbardi [UNESP] Nuñez, Karen Vanessa Munive Dantas, Stéfani Thais Alves [UNESP] Faganello, Caroline [UNESP] Juliano, Lara Cristina Bastos [UNESP] Tiba-Casas, Monique Ribeiro Pantoja, José C.F. [UNESP] Amarante, Ariadne Ferreira Júnior, Ary Fernandes [UNESP] Rall, Vera Lúcia Mores [UNESP] Silva, Nathália Cristina Cirone |
author_role |
author |
author2 |
da Silva, André Zimbardi [UNESP] Nuñez, Karen Vanessa Munive Dantas, Stéfani Thais Alves [UNESP] Faganello, Caroline [UNESP] Juliano, Lara Cristina Bastos [UNESP] Tiba-Casas, Monique Ribeiro Pantoja, José C.F. [UNESP] Amarante, Ariadne Ferreira Júnior, Ary Fernandes [UNESP] Rall, Vera Lúcia Mores [UNESP] Silva, Nathália Cristina Cirone |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) Adolfo Lutz Institute |
dc.contributor.author.fl_str_mv |
Morasi, Rafaela Martins da Silva, André Zimbardi [UNESP] Nuñez, Karen Vanessa Munive Dantas, Stéfani Thais Alves [UNESP] Faganello, Caroline [UNESP] Juliano, Lara Cristina Bastos [UNESP] Tiba-Casas, Monique Ribeiro Pantoja, José C.F. [UNESP] Amarante, Ariadne Ferreira Júnior, Ary Fernandes [UNESP] Rall, Vera Lúcia Mores [UNESP] Silva, Nathália Cristina Cirone |
dc.subject.por.fl_str_mv |
ESBL Foodborne disease Multidrug resistance Public health S. Heidelberg Virulence |
topic |
ESBL Foodborne disease Multidrug resistance Public health S. Heidelberg Virulence |
description |
Foodborne infections caused by Salmonella have been linked to a variety of poultry products. The aim of this study was to compare the molecular profile of virulence genes considering different serotypes of Salmonella, isolates were from chicken breast sampled during the last two decades (1999 to 2010 and 2011 to 2018). The resistance to antimicrobials was also evaluated, establishing a comparative epidemiological parameter on the pathogenic potential on this bacterium over time. We tested 238 Salmonella isolates, and 18 different serotypes were observed. These being S. Enteritidis (42.3%, 58/137) and S. Ohio (28.3%, 36/137), the most frequent in the first decade; and S. Heidelberg (25.7%, 26/101) and S. Typhimurium (21.8%, 22/101), in the second. We found four (1.68%) multidrug resistant isolates from the first decade and 28 (11.76%) in the second. All extended spectrum beta-lactamase (ESBL) positive isolates belonged to the S. Heidelberg serotype, and were also detected in the second decade. Considering the nine different antimicrobial classes tested, an increase in the number of resistant isolates was observed over time: from five classes with resistant isolates in the first decade to eight classes in the second, with cefotaxime being the antimicrobial with the highest number of resistant isolates in both decades. All isolates (100%) presented the invA, sitC and tolC genes. In sequence, the most frequent genes were flgL (99.6%), sopB (98.3%), flgK (97.9%), fljB (96.6%), sipA (94.9%), sipB (88.6%), sifA (86.4%), sipD (66.1%), ssaR (51.3%), sopD (37.3%) and spvB (34.3%) was the least frequent; and 13 isolates showing all 14 virulence genes investigated. The ability of these isolates to resist certain antimicrobials, and to express genes encoding virulence factors, reinforce their marked pathogenic potential; while the possibility to trigger diseases in humans through the food chain is a serious public health threat through. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T13:23:44Z 2023-07-29T13:23:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.foodres.2022.111955 Food Research International, v. 162. 1873-7145 0963-9969 http://hdl.handle.net/11449/247710 10.1016/j.foodres.2022.111955 2-s2.0-85139291262 |
url |
http://dx.doi.org/10.1016/j.foodres.2022.111955 http://hdl.handle.net/11449/247710 |
identifier_str_mv |
Food Research International, v. 162. 1873-7145 0963-9969 10.1016/j.foodres.2022.111955 2-s2.0-85139291262 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Research International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128275409534976 |