Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.snr.2022.100085 http://hdl.handle.net/11449/223481 |
Resumo: | Dopamine (DA) is a neurotransmitter with multiple functions in the central nervous system, and whether found in low levels, activation of biological defense system and physiological activity is influenced. DA at abnormal levels in the body may cause neurodegenerative illnesses such as Parkinson's disease or schizophrenia, so monitoring DA level and intervening as soon as possible is highly desirable. In this work, an electrochemical biosensor was produced on a gold substrate using self-assembled monolayer of 11 mercaptoundecanoic acid (11-Mua), followed by Layer by layer (LbL) technique. For this, a layer of cationic polyelectrolyte poly(ethyleneimine) (PEI), and finally a layer of gold nanoparticles stabilized with glutathione and functionalized with flavin adenine dinucleotide (FAD). FAD is a cofactor present in active site of monoamine oxidase enzyme and confers selectivity to the biosensor. Electrochemical biosensor was characterized by UV-Vis and FTIR spectroscopies, atomic force microscopy (AFM), and electrochemical techniques. Electrochemical detection was performed by differential pulse voltammetry (DPV) in PBS buffer showed a linear range of detection from 0.8 to 8.0 µmol L−1, sensitivity was 1.25 µA/µmol L−1 cm−2, limits of detection and quantification calculated were respectively 0.525 µmol L−1 and 1.75 µmol L−1. Biosensor showed recovery values between 85 and 90% in the study with human urine. According to sensor performance, gold nanoparticles and FAD could be used in electrochemical detection due to their high sensitivity and low detection limit. |
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Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamineDopamineElectrochemical biosensorFAD-gold nanoparticlesLayer-by-layer filmDopamine (DA) is a neurotransmitter with multiple functions in the central nervous system, and whether found in low levels, activation of biological defense system and physiological activity is influenced. DA at abnormal levels in the body may cause neurodegenerative illnesses such as Parkinson's disease or schizophrenia, so monitoring DA level and intervening as soon as possible is highly desirable. In this work, an electrochemical biosensor was produced on a gold substrate using self-assembled monolayer of 11 mercaptoundecanoic acid (11-Mua), followed by Layer by layer (LbL) technique. For this, a layer of cationic polyelectrolyte poly(ethyleneimine) (PEI), and finally a layer of gold nanoparticles stabilized with glutathione and functionalized with flavin adenine dinucleotide (FAD). FAD is a cofactor present in active site of monoamine oxidase enzyme and confers selectivity to the biosensor. Electrochemical biosensor was characterized by UV-Vis and FTIR spectroscopies, atomic force microscopy (AFM), and electrochemical techniques. Electrochemical detection was performed by differential pulse voltammetry (DPV) in PBS buffer showed a linear range of detection from 0.8 to 8.0 µmol L−1, sensitivity was 1.25 µA/µmol L−1 cm−2, limits of detection and quantification calculated were respectively 0.525 µmol L−1 and 1.75 µmol L−1. Biosensor showed recovery values between 85 and 90% in the study with human urine. According to sensor performance, gold nanoparticles and FAD could be used in electrochemical detection due to their high sensitivity and low detection limit.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Center of Science and Technology for Sustainability Federal University of São Carlos (UFSCar), SPInstitute of Science and Technology São Paulo State University (UNESP), SPInstitute of Science and Technology São Paulo State University (UNESP), SPFAPESP: 2015/05283-6FAPESP: 2016/19387-0Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Medrades, Jennifer de PontesMaciel, Cristiane C. [UNESP]Moraes, Ariana de SouzaLeite, Fábio de LimaFerreira, Marystela [UNESP]2022-04-28T19:50:53Z2022-04-28T19:50:53Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.snr.2022.100085Sensors and Actuators Reports, v. 4.2666-0539http://hdl.handle.net/11449/22348110.1016/j.snr.2022.1000852-s2.0-85124793427Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSensors and Actuators Reportsinfo:eu-repo/semantics/openAccess2022-04-28T19:50:53Zoai:repositorio.unesp.br:11449/223481Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:50:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine |
title |
Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine |
spellingShingle |
Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine Medrades, Jennifer de Pontes Dopamine Electrochemical biosensor FAD-gold nanoparticles Layer-by-layer film |
title_short |
Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine |
title_full |
Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine |
title_fullStr |
Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine |
title_full_unstemmed |
Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine |
title_sort |
Flavin adenine dinucleotide functionalized gold nanoparticles for the electrochemical detection of dopamine |
author |
Medrades, Jennifer de Pontes |
author_facet |
Medrades, Jennifer de Pontes Maciel, Cristiane C. [UNESP] Moraes, Ariana de Souza Leite, Fábio de Lima Ferreira, Marystela [UNESP] |
author_role |
author |
author2 |
Maciel, Cristiane C. [UNESP] Moraes, Ariana de Souza Leite, Fábio de Lima Ferreira, Marystela [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Medrades, Jennifer de Pontes Maciel, Cristiane C. [UNESP] Moraes, Ariana de Souza Leite, Fábio de Lima Ferreira, Marystela [UNESP] |
dc.subject.por.fl_str_mv |
Dopamine Electrochemical biosensor FAD-gold nanoparticles Layer-by-layer film |
topic |
Dopamine Electrochemical biosensor FAD-gold nanoparticles Layer-by-layer film |
description |
Dopamine (DA) is a neurotransmitter with multiple functions in the central nervous system, and whether found in low levels, activation of biological defense system and physiological activity is influenced. DA at abnormal levels in the body may cause neurodegenerative illnesses such as Parkinson's disease or schizophrenia, so monitoring DA level and intervening as soon as possible is highly desirable. In this work, an electrochemical biosensor was produced on a gold substrate using self-assembled monolayer of 11 mercaptoundecanoic acid (11-Mua), followed by Layer by layer (LbL) technique. For this, a layer of cationic polyelectrolyte poly(ethyleneimine) (PEI), and finally a layer of gold nanoparticles stabilized with glutathione and functionalized with flavin adenine dinucleotide (FAD). FAD is a cofactor present in active site of monoamine oxidase enzyme and confers selectivity to the biosensor. Electrochemical biosensor was characterized by UV-Vis and FTIR spectroscopies, atomic force microscopy (AFM), and electrochemical techniques. Electrochemical detection was performed by differential pulse voltammetry (DPV) in PBS buffer showed a linear range of detection from 0.8 to 8.0 µmol L−1, sensitivity was 1.25 µA/µmol L−1 cm−2, limits of detection and quantification calculated were respectively 0.525 µmol L−1 and 1.75 µmol L−1. Biosensor showed recovery values between 85 and 90% in the study with human urine. According to sensor performance, gold nanoparticles and FAD could be used in electrochemical detection due to their high sensitivity and low detection limit. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:50:53Z 2022-04-28T19:50:53Z 2022-11-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.snr.2022.100085 Sensors and Actuators Reports, v. 4. 2666-0539 http://hdl.handle.net/11449/223481 10.1016/j.snr.2022.100085 2-s2.0-85124793427 |
url |
http://dx.doi.org/10.1016/j.snr.2022.100085 http://hdl.handle.net/11449/223481 |
identifier_str_mv |
Sensors and Actuators Reports, v. 4. 2666-0539 10.1016/j.snr.2022.100085 2-s2.0-85124793427 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sensors and Actuators Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965749110898688 |