Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1751-0147-54-1 http://hdl.handle.net/11449/14808 |
Resumo: | Background: Proximal cytoplasmic droplets (PCDs), a remnant of germ cell cytoplasm, are common non-specific morphological defects in bovine semen. This study evaluated the effect of higher percentages of PCDs on the quality of frozen-thawed bovine semen, embryo production and early embryo development.Methods: Three ejaculates from each of five (group 1: PCD <= 1%, control) and eight adult Bos indicus bulls (group 2: PCD >= 24%) were analysed. Semen samples were examined for: post-thaw motility, vigour of movement, concentration, sperm morphology, slow thermoresistance test (STT), membrane integrity, acrosome status, mitochondrial function using fluorescent probes association (FITC-PSA, PI and JC-1) and sperm chromatin integrity using acridine orange assay. Two bulls from group 2, with 28.5% and 48.5% PCD, respectively, and three bulls from the control group, each with 0% PCD, were selected for IVF (in vitro fertilisation).Results: Semen analyses revealed a significant correlation (P < 0.01) between increased rates of PCD and sperm quality traits. Nevertheless, no differences were observed in sperm motility and vigour either before or after the STT or in the percentage of intact acrosomes (analysed by differential interference contrast microscopy (DIC) after STT), but membrane integrity, acrosome status (evaluated with FITC-PSA staining method after thawing) and mitochondrial function were reduced, when compared with group 1 (P < 0.05). The higher incidence of PCD was positively correlated to chromatin damage, especially after three hours of incubation at 37 degrees C. IVF showed similar results for bull C2 (group 1, control) and bull P2 (group 2, group with higher PCDs).Conclusion: Higher PCD levels influenced spermatozoa quality traits. IVF and embryo development data showed that cleavage, blastocyst formation and blastocyst hatching may have been influenced by the interaction of morphology traits and individual bull effects. |
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Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoasperm morphologyproximal cytoplasmic dropletsIVFfluorescent probesBackground: Proximal cytoplasmic droplets (PCDs), a remnant of germ cell cytoplasm, are common non-specific morphological defects in bovine semen. This study evaluated the effect of higher percentages of PCDs on the quality of frozen-thawed bovine semen, embryo production and early embryo development.Methods: Three ejaculates from each of five (group 1: PCD <= 1%, control) and eight adult Bos indicus bulls (group 2: PCD >= 24%) were analysed. Semen samples were examined for: post-thaw motility, vigour of movement, concentration, sperm morphology, slow thermoresistance test (STT), membrane integrity, acrosome status, mitochondrial function using fluorescent probes association (FITC-PSA, PI and JC-1) and sperm chromatin integrity using acridine orange assay. Two bulls from group 2, with 28.5% and 48.5% PCD, respectively, and three bulls from the control group, each with 0% PCD, were selected for IVF (in vitro fertilisation).Results: Semen analyses revealed a significant correlation (P < 0.01) between increased rates of PCD and sperm quality traits. Nevertheless, no differences were observed in sperm motility and vigour either before or after the STT or in the percentage of intact acrosomes (analysed by differential interference contrast microscopy (DIC) after STT), but membrane integrity, acrosome status (evaluated with FITC-PSA staining method after thawing) and mitochondrial function were reduced, when compared with group 1 (P < 0.05). The higher incidence of PCD was positively correlated to chromatin damage, especially after three hours of incubation at 37 degrees C. IVF showed similar results for bull C2 (group 1, control) and bull P2 (group 2, group with higher PCDs).Conclusion: Higher PCD levels influenced spermatozoa quality traits. IVF and embryo development data showed that cleavage, blastocyst formation and blastocyst hatching may have been influenced by the interaction of morphology traits and individual bull effects.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista UNESP, FMVA, Fac Vet Med, Dept Clin Surg & Anim Reprod, Aracatuba, BrazilUniv Estadual Paulista UNESP, Dept Support Prod & Anim Hlth, Aracatuba, BrazilCRVLagoa, Sertaozinho, BrazilUniv Estadual Paulista UNESP, FMVA, Fac Vet Med, Dept Clin Surg & Anim Reprod, Aracatuba, BrazilUniv Estadual Paulista UNESP, Dept Support Prod & Anim Hlth, Aracatuba, BrazilFAPESP: 06/03537-1Biomed Central Ltd.Universidade Estadual Paulista (Unesp)CRVLagoaCarreira, Janaina T. [UNESP]Mingoti, Gisele Zoccal [UNESP]Rodrigues, Lucia H.Silva, CarlosPerri, Silvia Helena Venturoli [UNESP]Koivisto, Marion B. [UNESP]2013-09-30T18:25:10Z2014-05-20T13:42:35Z2013-09-30T18:25:10Z2014-05-20T13:42:35Z2012-01-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1186/1751-0147-54-1Acta Veterinaria Scandinavica. London: Biomed Central Ltd., v. 54, p. 7, 2012.0044-605Xhttp://hdl.handle.net/11449/1480810.1186/1751-0147-54-1WOS:000300973900001WOS000300973900001.pdf419195176433476608108375982026340000-0002-3059-4458Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Veterinaria Scandinavica1.497info:eu-repo/semantics/openAccess2024-09-04T19:15:26Zoai:repositorio.unesp.br:11449/14808Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa |
title |
Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa |
spellingShingle |
Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa Carreira, Janaina T. [UNESP] sperm morphology proximal cytoplasmic droplets IVF fluorescent probes |
title_short |
Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa |
title_full |
Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa |
title_fullStr |
Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa |
title_full_unstemmed |
Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa |
title_sort |
Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa |
author |
Carreira, Janaina T. [UNESP] |
author_facet |
Carreira, Janaina T. [UNESP] Mingoti, Gisele Zoccal [UNESP] Rodrigues, Lucia H. Silva, Carlos Perri, Silvia Helena Venturoli [UNESP] Koivisto, Marion B. [UNESP] |
author_role |
author |
author2 |
Mingoti, Gisele Zoccal [UNESP] Rodrigues, Lucia H. Silva, Carlos Perri, Silvia Helena Venturoli [UNESP] Koivisto, Marion B. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) CRVLagoa |
dc.contributor.author.fl_str_mv |
Carreira, Janaina T. [UNESP] Mingoti, Gisele Zoccal [UNESP] Rodrigues, Lucia H. Silva, Carlos Perri, Silvia Helena Venturoli [UNESP] Koivisto, Marion B. [UNESP] |
dc.subject.por.fl_str_mv |
sperm morphology proximal cytoplasmic droplets IVF fluorescent probes |
topic |
sperm morphology proximal cytoplasmic droplets IVF fluorescent probes |
description |
Background: Proximal cytoplasmic droplets (PCDs), a remnant of germ cell cytoplasm, are common non-specific morphological defects in bovine semen. This study evaluated the effect of higher percentages of PCDs on the quality of frozen-thawed bovine semen, embryo production and early embryo development.Methods: Three ejaculates from each of five (group 1: PCD <= 1%, control) and eight adult Bos indicus bulls (group 2: PCD >= 24%) were analysed. Semen samples were examined for: post-thaw motility, vigour of movement, concentration, sperm morphology, slow thermoresistance test (STT), membrane integrity, acrosome status, mitochondrial function using fluorescent probes association (FITC-PSA, PI and JC-1) and sperm chromatin integrity using acridine orange assay. Two bulls from group 2, with 28.5% and 48.5% PCD, respectively, and three bulls from the control group, each with 0% PCD, were selected for IVF (in vitro fertilisation).Results: Semen analyses revealed a significant correlation (P < 0.01) between increased rates of PCD and sperm quality traits. Nevertheless, no differences were observed in sperm motility and vigour either before or after the STT or in the percentage of intact acrosomes (analysed by differential interference contrast microscopy (DIC) after STT), but membrane integrity, acrosome status (evaluated with FITC-PSA staining method after thawing) and mitochondrial function were reduced, when compared with group 1 (P < 0.05). The higher incidence of PCD was positively correlated to chromatin damage, especially after three hours of incubation at 37 degrees C. IVF showed similar results for bull C2 (group 1, control) and bull P2 (group 2, group with higher PCDs).Conclusion: Higher PCD levels influenced spermatozoa quality traits. IVF and embryo development data showed that cleavage, blastocyst formation and blastocyst hatching may have been influenced by the interaction of morphology traits and individual bull effects. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-12 2013-09-30T18:25:10Z 2013-09-30T18:25:10Z 2014-05-20T13:42:35Z 2014-05-20T13:42:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1751-0147-54-1 Acta Veterinaria Scandinavica. London: Biomed Central Ltd., v. 54, p. 7, 2012. 0044-605X http://hdl.handle.net/11449/14808 10.1186/1751-0147-54-1 WOS:000300973900001 WOS000300973900001.pdf 4191951764334766 0810837598202634 0000-0002-3059-4458 |
url |
http://dx.doi.org/10.1186/1751-0147-54-1 http://hdl.handle.net/11449/14808 |
identifier_str_mv |
Acta Veterinaria Scandinavica. London: Biomed Central Ltd., v. 54, p. 7, 2012. 0044-605X 10.1186/1751-0147-54-1 WOS:000300973900001 WOS000300973900001.pdf 4191951764334766 0810837598202634 0000-0002-3059-4458 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Veterinaria Scandinavica 1.497 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021383567048704 |