Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus

Detalhes bibliográficos
Autor(a) principal: Espinha, Luciana M. [UNESP]
Data de Publicação: 1999
Outros Autores: Gaspar, José O. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/224137
Resumo: Electron microscopy and immunolabelling with antiserum specific to cucumber mosaic virus coat protein were used to examine tobacco leaf cells infected by cucumber mosaic virus isolated from Catharanthus roseus (CMV-Cr). Crystalline and amorphous inclusions in the vacuoles were the most obvious cytological modifications seen. Immunogold labelling indicated that the crystalline inclusion was made up of virus particles and amorphous inclusions contained coat protein. Rows of CMV-Cr particles were found between membranes of dictyosomes, but membranous bodies and tonoplast-associated vesicles were not evident. Virus particles and/or free coat protein were easily detected in the cytoplasm by immunolabelling. No gold labelling was found within nuclei, chloroplasts and mitochondria.
id UNSP_7b03a479df9fec0bf01868348989ae2d
oai_identifier_str oai:repositorio.unesp.br:11449/224137
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseusCapsid proteinCucumovirusElectron microscopyImmunolabellingVinca roseaElectron microscopy and immunolabelling with antiserum specific to cucumber mosaic virus coat protein were used to examine tobacco leaf cells infected by cucumber mosaic virus isolated from Catharanthus roseus (CMV-Cr). Crystalline and amorphous inclusions in the vacuoles were the most obvious cytological modifications seen. Immunogold labelling indicated that the crystalline inclusion was made up of virus particles and amorphous inclusions contained coat protein. Rows of CMV-Cr particles were found between membranes of dictyosomes, but membranous bodies and tonoplast-associated vesicles were not evident. Virus particles and/or free coat protein were easily detected in the cytoplasm by immunolabelling. No gold labelling was found within nuclei, chloroplasts and mitochondria.Departamento de Botânica IBILCE/UNESP, CP 136, 15054-000, Sao Jose do Rio Preto, SPDepartamento de Botânica IBILCE/UNESP, CP 136, 15054-000, Sao Jose do Rio Preto, SPUniversidade Estadual Paulista (UNESP)Espinha, Luciana M. [UNESP]Gaspar, José O. [UNESP]2022-04-28T19:54:54Z2022-04-28T19:54:54Z1999-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article119-126Cytobios, v. 1999, n. 394, p. 119-126, 1999.0011-4529http://hdl.handle.net/11449/2241372-s2.0-0033254896Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytobiosinfo:eu-repo/semantics/openAccess2022-04-28T19:54:54Zoai:repositorio.unesp.br:11449/224137Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:45:44.872753Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
title Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
spellingShingle Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
Espinha, Luciana M. [UNESP]
Capsid protein
Cucumovirus
Electron microscopy
Immunolabelling
Vinca rosea
title_short Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
title_full Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
title_fullStr Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
title_full_unstemmed Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
title_sort Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
author Espinha, Luciana M. [UNESP]
author_facet Espinha, Luciana M. [UNESP]
Gaspar, José O. [UNESP]
author_role author
author2 Gaspar, José O. [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Espinha, Luciana M. [UNESP]
Gaspar, José O. [UNESP]
dc.subject.por.fl_str_mv Capsid protein
Cucumovirus
Electron microscopy
Immunolabelling
Vinca rosea
topic Capsid protein
Cucumovirus
Electron microscopy
Immunolabelling
Vinca rosea
description Electron microscopy and immunolabelling with antiserum specific to cucumber mosaic virus coat protein were used to examine tobacco leaf cells infected by cucumber mosaic virus isolated from Catharanthus roseus (CMV-Cr). Crystalline and amorphous inclusions in the vacuoles were the most obvious cytological modifications seen. Immunogold labelling indicated that the crystalline inclusion was made up of virus particles and amorphous inclusions contained coat protein. Rows of CMV-Cr particles were found between membranes of dictyosomes, but membranous bodies and tonoplast-associated vesicles were not evident. Virus particles and/or free coat protein were easily detected in the cytoplasm by immunolabelling. No gold labelling was found within nuclei, chloroplasts and mitochondria.
publishDate 1999
dc.date.none.fl_str_mv 1999-12-01
2022-04-28T19:54:54Z
2022-04-28T19:54:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Cytobios, v. 1999, n. 394, p. 119-126, 1999.
0011-4529
http://hdl.handle.net/11449/224137
2-s2.0-0033254896
identifier_str_mv Cytobios, v. 1999, n. 394, p. 119-126, 1999.
0011-4529
2-s2.0-0033254896
url http://hdl.handle.net/11449/224137
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cytobios
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 119-126
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128975513321472

Registros relacionados