Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/jac/dky335 http://hdl.handle.net/11449/186323 |
Resumo: | Objectives: Brazil is the greatest exporter of chicken meat (CM) in the world. It is of utmost importance to monitor resistance to extended-spectrum cephalosporins (ESCs) in this sector because resistance to ESCs in Escherichia coli isolated from food-producing animals may contaminate humans through the food chain. Thus, the aim of this study was to characterize and compare ESC-resistant E. coli isolated from chickens and retail CM produced in south-eastern Brazil. Methods: Five CM samples and 117 chicken cloacal swabs (CCSs) were inoculated on MacConkey agar supplemented with cefotaxime. Presumptive E. coli colonies were identified and antimicrobial susceptibility was tested. Virulence and acquired bla(ESBL) and bla(AmpC) genes were sought and genetic environments characterized. Isolates were typed by phylogenetic grouping, XbaI-PFGE and MLST. Results: All five CM samples and 36 CCSs (30.8%) were positive for the presence of ESC-resistant E. coli, leading to the selection of 58 resistant isolates. ESC resistance was mostly due to the presence of the chromosome-encoded bla(CTX-M-2) gene, but plasmid-mediated bla(CTX-M-2), bla(CTX-M-8), bla(CTX-M-15), bla(CTX-M-55) and bla(CMY-2) were also detected. Multireplicon plasmids were sporadically identified, such as IncHI2/P-bla(CTX-M-2) and IncFII/N-bla(CTX-M-55). Phylogroup D predominated, while PFGE and MLST revealed a high genetic diversity. Conclusions: Live Brazilian chickens and CM act as reservoirs of ESC-resistant E. coli and resistance genes are located on highly diverse genetic determinants. Potentially pathogenic strains, which may represent a threat to human health and a source of environmental contamination, were also identified. Active surveillance is therefore essential in Brazil's chicken production line. |
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Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineagesObjectives: Brazil is the greatest exporter of chicken meat (CM) in the world. It is of utmost importance to monitor resistance to extended-spectrum cephalosporins (ESCs) in this sector because resistance to ESCs in Escherichia coli isolated from food-producing animals may contaminate humans through the food chain. Thus, the aim of this study was to characterize and compare ESC-resistant E. coli isolated from chickens and retail CM produced in south-eastern Brazil. Methods: Five CM samples and 117 chicken cloacal swabs (CCSs) were inoculated on MacConkey agar supplemented with cefotaxime. Presumptive E. coli colonies were identified and antimicrobial susceptibility was tested. Virulence and acquired bla(ESBL) and bla(AmpC) genes were sought and genetic environments characterized. Isolates were typed by phylogenetic grouping, XbaI-PFGE and MLST. Results: All five CM samples and 36 CCSs (30.8%) were positive for the presence of ESC-resistant E. coli, leading to the selection of 58 resistant isolates. ESC resistance was mostly due to the presence of the chromosome-encoded bla(CTX-M-2) gene, but plasmid-mediated bla(CTX-M-2), bla(CTX-M-8), bla(CTX-M-15), bla(CTX-M-55) and bla(CMY-2) were also detected. Multireplicon plasmids were sporadically identified, such as IncHI2/P-bla(CTX-M-2) and IncFII/N-bla(CTX-M-55). Phylogroup D predominated, while PFGE and MLST revealed a high genetic diversity. Conclusions: Live Brazilian chickens and CM act as reservoirs of ESC-resistant E. coli and resistance genes are located on highly diverse genetic determinants. Potentially pathogenic strains, which may represent a threat to human health and a source of environmental contamination, were also identified. Active surveillance is therefore essential in Brazil's chicken production line.French Agency for Food, Environmental and Occupational Health Safety (ANSES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, BrazilUniv Claude Bernard Lyon 1, ANSES, Unite Antibioresistance & Virulence Bacteriennes, Lyon, FranceFac Med Sao Jose Do Rio Preto, Sao Jose Do Rio Preto, BrazilHosp Base Sao Jose Do Rio Preto, Sao Jose Do Rio Preto, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, BrazilFAPESP: 2014/17184-0CAPES: 99999.009932/2014-03Oxford Univ PressUniversidade Estadual Paulista (Unesp)Univ Claude Bernard Lyon 1Universidade de São Paulo (USP)Hosp Base Sao Jose Do Rio PretoCasella, Tiago [UNESP]Haenni, MarisaMadela, Naiady KonnoAndrade, Leticia Kellen dePradela, Leticia KalirAndrade, Leonardo Neves deCosta Darini, Ana Lucia daMadec, Jean-YvesLelles Nogueira, Mara Correa2019-10-04T18:37:12Z2019-10-04T18:37:12Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3293-3297http://dx.doi.org/10.1093/jac/dky335Journal Of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 73, n. 12, p. 3293-3297, 2018.0305-7453http://hdl.handle.net/11449/18632310.1093/jac/dky335WOS:000452916600010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Antimicrobial Chemotherapyinfo:eu-repo/semantics/openAccess2021-10-23T05:33:01Zoai:repositorio.unesp.br:11449/186323Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T05:33:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages |
title |
Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages |
spellingShingle |
Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages Casella, Tiago [UNESP] |
title_short |
Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages |
title_full |
Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages |
title_fullStr |
Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages |
title_full_unstemmed |
Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages |
title_sort |
Extended-spectrum cephalosporin-resistant Escherichia coli isolated from chickens and chicken meat in Brazil is associated with rare and complex resistance plasmids and pandemic ST lineages |
author |
Casella, Tiago [UNESP] |
author_facet |
Casella, Tiago [UNESP] Haenni, Marisa Madela, Naiady Konno Andrade, Leticia Kellen de Pradela, Leticia Kalir Andrade, Leonardo Neves de Costa Darini, Ana Lucia da Madec, Jean-Yves Lelles Nogueira, Mara Correa |
author_role |
author |
author2 |
Haenni, Marisa Madela, Naiady Konno Andrade, Leticia Kellen de Pradela, Leticia Kalir Andrade, Leonardo Neves de Costa Darini, Ana Lucia da Madec, Jean-Yves Lelles Nogueira, Mara Correa |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Claude Bernard Lyon 1 Universidade de São Paulo (USP) Hosp Base Sao Jose Do Rio Preto |
dc.contributor.author.fl_str_mv |
Casella, Tiago [UNESP] Haenni, Marisa Madela, Naiady Konno Andrade, Leticia Kellen de Pradela, Leticia Kalir Andrade, Leonardo Neves de Costa Darini, Ana Lucia da Madec, Jean-Yves Lelles Nogueira, Mara Correa |
description |
Objectives: Brazil is the greatest exporter of chicken meat (CM) in the world. It is of utmost importance to monitor resistance to extended-spectrum cephalosporins (ESCs) in this sector because resistance to ESCs in Escherichia coli isolated from food-producing animals may contaminate humans through the food chain. Thus, the aim of this study was to characterize and compare ESC-resistant E. coli isolated from chickens and retail CM produced in south-eastern Brazil. Methods: Five CM samples and 117 chicken cloacal swabs (CCSs) were inoculated on MacConkey agar supplemented with cefotaxime. Presumptive E. coli colonies were identified and antimicrobial susceptibility was tested. Virulence and acquired bla(ESBL) and bla(AmpC) genes were sought and genetic environments characterized. Isolates were typed by phylogenetic grouping, XbaI-PFGE and MLST. Results: All five CM samples and 36 CCSs (30.8%) were positive for the presence of ESC-resistant E. coli, leading to the selection of 58 resistant isolates. ESC resistance was mostly due to the presence of the chromosome-encoded bla(CTX-M-2) gene, but plasmid-mediated bla(CTX-M-2), bla(CTX-M-8), bla(CTX-M-15), bla(CTX-M-55) and bla(CMY-2) were also detected. Multireplicon plasmids were sporadically identified, such as IncHI2/P-bla(CTX-M-2) and IncFII/N-bla(CTX-M-55). Phylogroup D predominated, while PFGE and MLST revealed a high genetic diversity. Conclusions: Live Brazilian chickens and CM act as reservoirs of ESC-resistant E. coli and resistance genes are located on highly diverse genetic determinants. Potentially pathogenic strains, which may represent a threat to human health and a source of environmental contamination, were also identified. Active surveillance is therefore essential in Brazil's chicken production line. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2019-10-04T18:37:12Z 2019-10-04T18:37:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/jac/dky335 Journal Of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 73, n. 12, p. 3293-3297, 2018. 0305-7453 http://hdl.handle.net/11449/186323 10.1093/jac/dky335 WOS:000452916600010 |
url |
http://dx.doi.org/10.1093/jac/dky335 http://hdl.handle.net/11449/186323 |
identifier_str_mv |
Journal Of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 73, n. 12, p. 3293-3297, 2018. 0305-7453 10.1093/jac/dky335 WOS:000452916600010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Antimicrobial Chemotherapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3293-3297 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803649411261136896 |