Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/2211-5463.12124 http://hdl.handle.net/11449/173600 |
Resumo: | Different isoforms of ataxin-2 are predicted in Drosophila and may underlie different cellular processes. Here, we validated the isoforms B and C of Drosophila ataxin-2 locus (dAtx2), which we found to be expressed in various tissues and at different levels during development. dAtx2-B mRNA was detected at low amounts during all developmental stages, whereas dAtx2-C mRNA levels increase by eightfold from L3 to pupal–adult stages. Higher amounts of dAtx2-B protein were detected in embryos, while dAtx2-C protein was also expressed in higher levels in pupal–adult stages, indicating post-transcriptional control for isoform B and transcription induction for isoform C, respectively. Moreover, in the fat body of L3 larvae dAtx2-C, but not dAtx2-B, accumulates in cytoplasmic foci that colocalize with sec23, a marker of endoplasmic reticulum exit sites (ERES). Interestingly, animals subjected to selective knockdown of dAtx2 in the larval fat body do not complete metamorphosis and die at the third larval stage or early puparium. Additionally, larvae knocked down for dAtx2, grown at 29 °C, are significantly smaller than control animals due to reduction in DNA replication and cell growth, which are consistent with the decreased levels of phosphorylated-AKT observed in the fat body. Based on the localization of ataxin-2 (dAtx2-C) in ERESs, and on the phenotypes observed by dAtx2 knockdown in the larval fat body, we speculate a possible role for this protein in processes that regulate ERES formation. These data provide new insights into the biological function of ataxin-2 with potential relevance to neurodegenerative diseases. |
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Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissuesdAtx2 knockdowndevelopmental regulationDrosophila ataxin-2 isoformslarval fat bodysubcellular localizationDifferent isoforms of ataxin-2 are predicted in Drosophila and may underlie different cellular processes. Here, we validated the isoforms B and C of Drosophila ataxin-2 locus (dAtx2), which we found to be expressed in various tissues and at different levels during development. dAtx2-B mRNA was detected at low amounts during all developmental stages, whereas dAtx2-C mRNA levels increase by eightfold from L3 to pupal–adult stages. Higher amounts of dAtx2-B protein were detected in embryos, while dAtx2-C protein was also expressed in higher levels in pupal–adult stages, indicating post-transcriptional control for isoform B and transcription induction for isoform C, respectively. Moreover, in the fat body of L3 larvae dAtx2-C, but not dAtx2-B, accumulates in cytoplasmic foci that colocalize with sec23, a marker of endoplasmic reticulum exit sites (ERES). Interestingly, animals subjected to selective knockdown of dAtx2 in the larval fat body do not complete metamorphosis and die at the third larval stage or early puparium. Additionally, larvae knocked down for dAtx2, grown at 29 °C, are significantly smaller than control animals due to reduction in DNA replication and cell growth, which are consistent with the decreased levels of phosphorylated-AKT observed in the fat body. Based on the localization of ataxin-2 (dAtx2-C) in ERESs, and on the phenotypes observed by dAtx2 knockdown in the larval fat body, we speculate a possible role for this protein in processes that regulate ERES formation. These data provide new insights into the biological function of ataxin-2 with potential relevance to neurodegenerative diseases.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Cellular and Molecular Biology Ribeirão Preto School of Medicine University of São PauloCenter of Biological Sciences State University of Londrina Campus Universitário LondrinaDepartment of Clinical Analysis Faculty of Pharmaceutical Sciences of Araraquara University of São Paulo State (UNESP), R. Expedicionários do Brasil, 1628Department of Clinical Analysis Faculty of Pharmaceutical Sciences of Araraquara University of São Paulo State (UNESP), R. Expedicionários do Brasil, 1628FAPESP: 2011/51630-9Universidade de São Paulo (USP)Universidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Vianna, Murilo Carlos BizamPoleto, Deise CristinaGomes, Paula FernandaValente, Valéria [UNESP]Paçó-Larson, Maria Luisa2018-12-11T17:06:44Z2018-12-11T17:06:44Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1040-1053application/pdfhttp://dx.doi.org/10.1002/2211-5463.12124FEBS Open Bio, v. 6, n. 11, p. 1040-1053, 2016.2211-5463http://hdl.handle.net/11449/17360010.1002/2211-5463.121242-s2.0-849911070942-s2.0-84991107094.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFEBS Open Bio0,884info:eu-repo/semantics/openAccess2024-06-21T15:19:32Zoai:repositorio.unesp.br:11449/173600Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:23:54.569642Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues |
title |
Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues |
spellingShingle |
Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues Vianna, Murilo Carlos Bizam dAtx2 knockdown developmental regulation Drosophila ataxin-2 isoforms larval fat body subcellular localization |
title_short |
Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues |
title_full |
Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues |
title_fullStr |
Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues |
title_full_unstemmed |
Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues |
title_sort |
Drosophila ataxin-2 gene encodes two differentially expressed isoforms and its function in larval fat body is crucial for development of peripheral tissues |
author |
Vianna, Murilo Carlos Bizam |
author_facet |
Vianna, Murilo Carlos Bizam Poleto, Deise Cristina Gomes, Paula Fernanda Valente, Valéria [UNESP] Paçó-Larson, Maria Luisa |
author_role |
author |
author2 |
Poleto, Deise Cristina Gomes, Paula Fernanda Valente, Valéria [UNESP] Paçó-Larson, Maria Luisa |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vianna, Murilo Carlos Bizam Poleto, Deise Cristina Gomes, Paula Fernanda Valente, Valéria [UNESP] Paçó-Larson, Maria Luisa |
dc.subject.por.fl_str_mv |
dAtx2 knockdown developmental regulation Drosophila ataxin-2 isoforms larval fat body subcellular localization |
topic |
dAtx2 knockdown developmental regulation Drosophila ataxin-2 isoforms larval fat body subcellular localization |
description |
Different isoforms of ataxin-2 are predicted in Drosophila and may underlie different cellular processes. Here, we validated the isoforms B and C of Drosophila ataxin-2 locus (dAtx2), which we found to be expressed in various tissues and at different levels during development. dAtx2-B mRNA was detected at low amounts during all developmental stages, whereas dAtx2-C mRNA levels increase by eightfold from L3 to pupal–adult stages. Higher amounts of dAtx2-B protein were detected in embryos, while dAtx2-C protein was also expressed in higher levels in pupal–adult stages, indicating post-transcriptional control for isoform B and transcription induction for isoform C, respectively. Moreover, in the fat body of L3 larvae dAtx2-C, but not dAtx2-B, accumulates in cytoplasmic foci that colocalize with sec23, a marker of endoplasmic reticulum exit sites (ERES). Interestingly, animals subjected to selective knockdown of dAtx2 in the larval fat body do not complete metamorphosis and die at the third larval stage or early puparium. Additionally, larvae knocked down for dAtx2, grown at 29 °C, are significantly smaller than control animals due to reduction in DNA replication and cell growth, which are consistent with the decreased levels of phosphorylated-AKT observed in the fat body. Based on the localization of ataxin-2 (dAtx2-C) in ERESs, and on the phenotypes observed by dAtx2 knockdown in the larval fat body, we speculate a possible role for this protein in processes that regulate ERES formation. These data provide new insights into the biological function of ataxin-2 with potential relevance to neurodegenerative diseases. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-12-11T17:06:44Z 2018-12-11T17:06:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/2211-5463.12124 FEBS Open Bio, v. 6, n. 11, p. 1040-1053, 2016. 2211-5463 http://hdl.handle.net/11449/173600 10.1002/2211-5463.12124 2-s2.0-84991107094 2-s2.0-84991107094.pdf |
url |
http://dx.doi.org/10.1002/2211-5463.12124 http://hdl.handle.net/11449/173600 |
identifier_str_mv |
FEBS Open Bio, v. 6, n. 11, p. 1040-1053, 2016. 2211-5463 10.1002/2211-5463.12124 2-s2.0-84991107094 2-s2.0-84991107094.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FEBS Open Bio 0,884 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1040-1053 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129516648792064 |