Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules18078095 http://hdl.handle.net/11449/75864 |
Resumo: | In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. © 2013 by the authors. |
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Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided studyAntifungal activityCandidaCell wallEllagitanninPlinia cauliflora (Myrtaceae)In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. © 2013 by the authors.Department of Drugs and Medicines School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista, Rodovia Araraquara-Jau, km 1, Araraquara, 14801-902, São PauloDepartment of Microbiology Institute of Medical Sciences University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZDDepartment of Organic Chemistry Institute of Chemistry UNESP-Univ Estadual Paulista, R. Francisco Degni s/n, Araraquara, 14800-900, São PauloDepartment of Pharmacy and Nutrition Centro de Ciências Agrárias Alto Universitário, Guararema, 29500-000, Alegre, ESDepartment of Drugs and Medicines School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista, Rodovia Araraquara-Jau, km 1, Araraquara, 14801-902, São PauloDepartment of Organic Chemistry Institute of Chemistry UNESP-Univ Estadual Paulista, R. Francisco Degni s/n, Araraquara, 14800-900, São PauloUniversidade Estadual Paulista (Unesp)University of AberdeenAlto UniversitárioSouza-Moreira, Tatiana M. [UNESP]Severi, Juliana A. [UNESP]Lee, KeunsookPreechasuth, KanyaSantos, Emerson [UNESP]Gow, Neil A.R.Munro, Carol A.Vilegas, Wagner [UNESP]Pietro, Rosemeire C.L.R. [UNESP]2014-05-27T11:29:53Z2014-05-27T11:29:53Z2013-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8095-8108application/pdfhttp://dx.doi.org/10.3390/molecules18078095Molecules, v. 18, n. 7, p. 8095-8108, 2013.1420-3049http://hdl.handle.net/11449/7586410.3390/molecules18078095WOS:0003303009000482-s2.0-848807958712-s2.0-84880795871.pdf0000-0003-3032-2556Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules3.0980,855info:eu-repo/semantics/openAccess2024-06-24T13:45:19Zoai:repositorio.unesp.br:11449/75864Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:52:04.597639Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study |
title |
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study |
spellingShingle |
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study Souza-Moreira, Tatiana M. [UNESP] Antifungal activity Candida Cell wall Ellagitannin Plinia cauliflora (Myrtaceae) |
title_short |
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study |
title_full |
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study |
title_fullStr |
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study |
title_full_unstemmed |
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study |
title_sort |
Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study |
author |
Souza-Moreira, Tatiana M. [UNESP] |
author_facet |
Souza-Moreira, Tatiana M. [UNESP] Severi, Juliana A. [UNESP] Lee, Keunsook Preechasuth, Kanya Santos, Emerson [UNESP] Gow, Neil A.R. Munro, Carol A. Vilegas, Wagner [UNESP] Pietro, Rosemeire C.L.R. [UNESP] |
author_role |
author |
author2 |
Severi, Juliana A. [UNESP] Lee, Keunsook Preechasuth, Kanya Santos, Emerson [UNESP] Gow, Neil A.R. Munro, Carol A. Vilegas, Wagner [UNESP] Pietro, Rosemeire C.L.R. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Aberdeen Alto Universitário |
dc.contributor.author.fl_str_mv |
Souza-Moreira, Tatiana M. [UNESP] Severi, Juliana A. [UNESP] Lee, Keunsook Preechasuth, Kanya Santos, Emerson [UNESP] Gow, Neil A.R. Munro, Carol A. Vilegas, Wagner [UNESP] Pietro, Rosemeire C.L.R. [UNESP] |
dc.subject.por.fl_str_mv |
Antifungal activity Candida Cell wall Ellagitannin Plinia cauliflora (Myrtaceae) |
topic |
Antifungal activity Candida Cell wall Ellagitannin Plinia cauliflora (Myrtaceae) |
description |
In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. © 2013 by the authors. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-01 2014-05-27T11:29:53Z 2014-05-27T11:29:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules18078095 Molecules, v. 18, n. 7, p. 8095-8108, 2013. 1420-3049 http://hdl.handle.net/11449/75864 10.3390/molecules18078095 WOS:000330300900048 2-s2.0-84880795871 2-s2.0-84880795871.pdf 0000-0003-3032-2556 |
url |
http://dx.doi.org/10.3390/molecules18078095 http://hdl.handle.net/11449/75864 |
identifier_str_mv |
Molecules, v. 18, n. 7, p. 8095-8108, 2013. 1420-3049 10.3390/molecules18078095 WOS:000330300900048 2-s2.0-84880795871 2-s2.0-84880795871.pdf 0000-0003-3032-2556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules 3.098 0,855 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8095-8108 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128574964629504 |