Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5433/1679-0359.2021v42n1p209 http://hdl.handle.net/11449/208228 |
Resumo: | This study aimed to evaluate the hematologic response and the serum and peritoneal fluid (PF) proteinogram of cattle affected by digestive diseases. Twenty-seven animals were distributed in two groups: GI (intestinal diseases) and GII (traumatic reticuloperitonitis, TRP). The animals were previously submitted to a physical exam. Subsequently, blood samples were collected to perform the complete blood count, determine the plasma protein and fibrinogen, and obtain the serum for proteinogram in polyacrylamide gel (SDS-PAGE). Simultaneously, PF was collected to perform physical and chemical evaluation and the electrophoretic profile (SDS-PAGE). ANOVA at the 5% probability level was used to compare the groups. The animals showed signs of apathy, dehydration, and gastrointestinal hypomotility in both groups. However, GI animals showed more significant clinical changes. The blood count of both groups (P > 0.05) showed leukocytosis due to neutrophilia and a regenerative left shift with hyperfibrinogenemia. The proteinogram of both body fluids allowed the identification of proteins albumin (ALB), transferrin (TRF), ceruloplasmin, haptoglobin, α1-acid glycoprotein (α1-AGP), MW 23000 Da, α1-antitrypsin, IgA, and IgG. The [PT] PF/[PT] blood serum ratio of each of the identified proteins increased, showing statistical differences between groups (P < 0.05) regarding PT, ALB, TRF, α1-AGP, and IgG values, with GI animals showing the highest ratio. Intestinal diseases and TRP triggered a systemic and local response characterized by clinical, hematological, and serum and PF proteinogram alterations. The proteins α1-GPA, haptoglobin, and TRF measured in PF were good inflammation biomarkers and useful as an auxiliary tool for the diagnosis and prognosis of digestive diseases in cattle. |
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Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitisResposta hematológica e proteinograma sérico e do líquido peritoneal de bovinos acometidos com enfermidades intestinais e reticuloperitonite traumáticaAbdominal fluidAcute-phase proteinsCowsDigestive diseasesSDS-PAGEThis study aimed to evaluate the hematologic response and the serum and peritoneal fluid (PF) proteinogram of cattle affected by digestive diseases. Twenty-seven animals were distributed in two groups: GI (intestinal diseases) and GII (traumatic reticuloperitonitis, TRP). The animals were previously submitted to a physical exam. Subsequently, blood samples were collected to perform the complete blood count, determine the plasma protein and fibrinogen, and obtain the serum for proteinogram in polyacrylamide gel (SDS-PAGE). Simultaneously, PF was collected to perform physical and chemical evaluation and the electrophoretic profile (SDS-PAGE). ANOVA at the 5% probability level was used to compare the groups. The animals showed signs of apathy, dehydration, and gastrointestinal hypomotility in both groups. However, GI animals showed more significant clinical changes. The blood count of both groups (P > 0.05) showed leukocytosis due to neutrophilia and a regenerative left shift with hyperfibrinogenemia. The proteinogram of both body fluids allowed the identification of proteins albumin (ALB), transferrin (TRF), ceruloplasmin, haptoglobin, α1-acid glycoprotein (α1-AGP), MW 23000 Da, α1-antitrypsin, IgA, and IgG. The [PT] PF/[PT] blood serum ratio of each of the identified proteins increased, showing statistical differences between groups (P < 0.05) regarding PT, ALB, TRF, α1-AGP, and IgG values, with GI animals showing the highest ratio. Intestinal diseases and TRP triggered a systemic and local response characterized by clinical, hematological, and serum and PF proteinogram alterations. The proteins α1-GPA, haptoglobin, and TRF measured in PF were good inflammation biomarkers and useful as an auxiliary tool for the diagnosis and prognosis of digestive diseases in cattle.Instituto Federal de Educação Ciência e Tecnologia do Amazonas Campus Manaus Zona Leste IFAM CMZLUniversidade Federal do Espírito Santo Campus Alegre UFESClínica de Bovinos de Garanhuns Campus Garanhuns Universidade Federal Rural de Pernambuco CBG UFRPEFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho UNESP Campus de JaboticabalFaculdade de Ciências Agrárias e Veterinárias UNESP Campus de JaboticabalDepartamento de Medicina Veterinária DMV UFRPEFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho UNESP Campus de JaboticabalFaculdade de Ciências Agrárias e Veterinárias UNESP Campus de JaboticabalCMZLUniversidade Federal do Espírito Santo (UFES)UFRPEUniversidade Estadual Paulista (Unesp)dos Santos, Jomel Franciscodo Rego, Rafael OtavianoBastos Afonso, José AugustoFagliari, José Jurandir [UNESP]Silva, Paulo Cesar [UNESP]Soares, Pierre Castrode Mendonça, Carla Lopes2021-06-25T11:08:37Z2021-06-25T11:08:37Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article209-228http://dx.doi.org/10.5433/1679-0359.2021v42n1p209Semina:Ciencias Agrarias, v. 42, n. 1, p. 209-228, 2021.1679-03591676-546Xhttp://hdl.handle.net/11449/20822810.5433/1679-0359.2021v42n1p2092-s2.0-85097490613Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina:Ciencias Agrariasinfo:eu-repo/semantics/openAccess2024-06-06T14:10:47Zoai:repositorio.unesp.br:11449/208228Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:32:58.352124Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis Resposta hematológica e proteinograma sérico e do líquido peritoneal de bovinos acometidos com enfermidades intestinais e reticuloperitonite traumática |
title |
Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis |
spellingShingle |
Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis dos Santos, Jomel Francisco Abdominal fluid Acute-phase proteins Cows Digestive diseases SDS-PAGE |
title_short |
Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis |
title_full |
Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis |
title_fullStr |
Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis |
title_full_unstemmed |
Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis |
title_sort |
Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis |
author |
dos Santos, Jomel Francisco |
author_facet |
dos Santos, Jomel Francisco do Rego, Rafael Otaviano Bastos Afonso, José Augusto Fagliari, José Jurandir [UNESP] Silva, Paulo Cesar [UNESP] Soares, Pierre Castro de Mendonça, Carla Lopes |
author_role |
author |
author2 |
do Rego, Rafael Otaviano Bastos Afonso, José Augusto Fagliari, José Jurandir [UNESP] Silva, Paulo Cesar [UNESP] Soares, Pierre Castro de Mendonça, Carla Lopes |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
CMZL Universidade Federal do Espírito Santo (UFES) UFRPE Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
dos Santos, Jomel Francisco do Rego, Rafael Otaviano Bastos Afonso, José Augusto Fagliari, José Jurandir [UNESP] Silva, Paulo Cesar [UNESP] Soares, Pierre Castro de Mendonça, Carla Lopes |
dc.subject.por.fl_str_mv |
Abdominal fluid Acute-phase proteins Cows Digestive diseases SDS-PAGE |
topic |
Abdominal fluid Acute-phase proteins Cows Digestive diseases SDS-PAGE |
description |
This study aimed to evaluate the hematologic response and the serum and peritoneal fluid (PF) proteinogram of cattle affected by digestive diseases. Twenty-seven animals were distributed in two groups: GI (intestinal diseases) and GII (traumatic reticuloperitonitis, TRP). The animals were previously submitted to a physical exam. Subsequently, blood samples were collected to perform the complete blood count, determine the plasma protein and fibrinogen, and obtain the serum for proteinogram in polyacrylamide gel (SDS-PAGE). Simultaneously, PF was collected to perform physical and chemical evaluation and the electrophoretic profile (SDS-PAGE). ANOVA at the 5% probability level was used to compare the groups. The animals showed signs of apathy, dehydration, and gastrointestinal hypomotility in both groups. However, GI animals showed more significant clinical changes. The blood count of both groups (P > 0.05) showed leukocytosis due to neutrophilia and a regenerative left shift with hyperfibrinogenemia. The proteinogram of both body fluids allowed the identification of proteins albumin (ALB), transferrin (TRF), ceruloplasmin, haptoglobin, α1-acid glycoprotein (α1-AGP), MW 23000 Da, α1-antitrypsin, IgA, and IgG. The [PT] PF/[PT] blood serum ratio of each of the identified proteins increased, showing statistical differences between groups (P < 0.05) regarding PT, ALB, TRF, α1-AGP, and IgG values, with GI animals showing the highest ratio. Intestinal diseases and TRP triggered a systemic and local response characterized by clinical, hematological, and serum and PF proteinogram alterations. The proteins α1-GPA, haptoglobin, and TRF measured in PF were good inflammation biomarkers and useful as an auxiliary tool for the diagnosis and prognosis of digestive diseases in cattle. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:08:37Z 2021-06-25T11:08:37Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5433/1679-0359.2021v42n1p209 Semina:Ciencias Agrarias, v. 42, n. 1, p. 209-228, 2021. 1679-0359 1676-546X http://hdl.handle.net/11449/208228 10.5433/1679-0359.2021v42n1p209 2-s2.0-85097490613 |
url |
http://dx.doi.org/10.5433/1679-0359.2021v42n1p209 http://hdl.handle.net/11449/208228 |
identifier_str_mv |
Semina:Ciencias Agrarias, v. 42, n. 1, p. 209-228, 2021. 1679-0359 1676-546X 10.5433/1679-0359.2021v42n1p209 2-s2.0-85097490613 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Semina:Ciencias Agrarias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
209-228 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129333435301888 |