The Influence of Cooper and Chromium Ions on the Production of Exopolysaccharide and Polyhydroxybutyrate by Rhizobium tropici LBMP-C01

• Autor(a) principal: Leonel, Tatiane Fernanda [UNESP]
• Data de Publicação: 2019
• Outros Autores: Moretto, Cristiane [UNESP], Castellane, Tereza Cristina Luque [UNESP], da Costa, Paulo Inácio [UNESP], Lemos, Eliana Gertrudes de Macedo [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1007/s10924-018-1359-4; http://hdl.handle.net/11449/188565
• topic: Exopolysaccharide; Heavy metals; Polyhydroxybutyrate; Rhizobia

The influence of bivalent copper (Cu 2+ ) and hexavalent chromium (Cr 6+ ) ions on exopolysaccharide (EPS) and polyhydroxybutyrate (PHB) production by the bacterium Rhizobium tropici LBMP-C01 was investigated. The partial structure of both biopolymers produced by rhizobia were characterized, and the quantification of exoR and exoZ gene expression by real-time polymerase chain reaction (Real-Time PCR) was also investigated. Results showed that the supplementation with Cu 2+ for 144 h induced an increase in the production of EPS by 48% and PHB by 46.66% compared to the control. Expression studies revealed that the expressed exoR and exoZ genes were affected by metal ion treatment. It can be stated that this particular rhizobial strain is a future potential candidates to produce super adsorbent quite useful for soil bioremediation.