Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba

Detalhes bibliográficos
Autor(a) principal: Obregon, Dasiel
Data de Publicação: 2018
Outros Autores: Corona, Belkis G., la Fuente, Jose de, Cabezas-Cruz, Alejandro, Goncalves, Luiz Ricardo [UNESP], Matos, Carlos Antonio [UNESP], Armas, Yasmani, Hinojosa, Yoandri, Alfonso, Pastor, Oliveira, Marcia C. S., Machado, Rosangela Z. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vprsr.2018.06.007
http://hdl.handle.net/11449/210554
Resumo: Water buffalo (Bubalus bubalis) is a potential reservoir for Anaplasma marginale in livestock ecosystems of tropical countries. However, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. In the present study, the reservoir competence of water buffalo for A. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of A. marginale strains from cattle and buffalo. Eight groups of cattle and water buffaloes were randomly selected from cohabiting herds in four livestock ecosystems of Cuba, together with two control groups from unrelated cattle and buffalo herds. A total of 180 adult animals (88 water buffalo and 92 cattle) were sampled. Rickettsemia in carrier animals was determined by quantitative real-time PCR. The rickettsemia (parasitemia) levels in cattle were higher than in buffaloes, however the rickettsemia in buffalo may be enough to infect R. microplus ticks. The genetic diversity of A. marginale was assessed by strain characterization and phylogenetic analysis of 27 mspl a gene sequences. The results showed genetic similarity among strains from cattle and water buffalo, suggesting the occurrence of cross-species transmission.
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spelling Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in CubaWater buffaloCattleA. marginaleRickettsemiaGenetic diversityCross-transmissionWater buffalo (Bubalus bubalis) is a potential reservoir for Anaplasma marginale in livestock ecosystems of tropical countries. However, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. In the present study, the reservoir competence of water buffalo for A. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of A. marginale strains from cattle and buffalo. Eight groups of cattle and water buffaloes were randomly selected from cohabiting herds in four livestock ecosystems of Cuba, together with two control groups from unrelated cattle and buffalo herds. A total of 180 adult animals (88 water buffalo and 92 cattle) were sampled. Rickettsemia in carrier animals was determined by quantitative real-time PCR. The rickettsemia (parasitemia) levels in cattle were higher than in buffaloes, however the rickettsemia in buffalo may be enough to infect R. microplus ticks. The genetic diversity of A. marginale was assessed by strain characterization and phylogenetic analysis of 27 mspl a gene sequences. The results showed genetic similarity among strains from cattle and water buffalo, suggesting the occurrence of cross-species transmission.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Priority Program for Animal and Plant Health, Ministry of Agriculture (MINAGRI), CubaConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Agr La Habana, Carretera Tapaste & Autopista Nacl, San Jose De Las Lajas 32700, Mayabeque, CubaCtr Nacl Sanidad Agr, Carretera Jamaica & Autopista Nacl, San Jose De Las Lajas 32700, Mayabeque, CubaInst Invest Recursos Cineget IREC CSIC UCLM JCCM, SaBio, Ciudad Real 13005, SpainOklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USAUniv Paris Est, ANSES, INRA, UMR BIPAR,Ecole Natl Vet Alfort, F-94700 Maisons Alfort, FranceUniv Estadual Paulista, Campus Jaboticabal, BR-14884900 Jaboticabal, SP, BrazilEmbrapa Pecuaria Sudeste, Rodovia Washington Luiz,Km 234, BR-13560970 Sao Carlos, SP, BrazilUniv Estadual Paulista, Campus Jaboticabal, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2012/21371-4National Priority Program for Animal and Plant Health, Ministry of Agriculture (MINAGRI), Cuba: P131LH003007CNPq: 302999/2013-2Elsevier B.V.Univ Agr La HabanaCtr Nacl Sanidad AgrInst Invest Recursos Cineget IREC CSIC UCLM JCCMOklahoma State UnivUniv Paris EstUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Obregon, DasielCorona, Belkis G.la Fuente, Jose deCabezas-Cruz, AlejandroGoncalves, Luiz Ricardo [UNESP]Matos, Carlos Antonio [UNESP]Armas, YasmaniHinojosa, YoandriAlfonso, PastorOliveira, Marcia C. S.Machado, Rosangela Z. [UNESP]2021-06-25T21:07:58Z2021-06-25T21:07:58Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article180-187http://dx.doi.org/10.1016/j.vprsr.2018.06.007Veterinary Parasitology- Regional Studies And Reports. Amsterdam: Elsevier, v. 13, p. 180-187, 2018.2405-9390http://hdl.handle.net/11449/21055410.1016/j.vprsr.2018.06.007WOS:000592468400034Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology- Regional Studies And Reportsinfo:eu-repo/semantics/openAccess2024-06-07T13:02:06Zoai:repositorio.unesp.br:11449/210554Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:35:03.679705Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
title Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
spellingShingle Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
Obregon, Dasiel
Water buffalo
Cattle
A. marginale
Rickettsemia
Genetic diversity
Cross-transmission
title_short Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
title_full Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
title_fullStr Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
title_full_unstemmed Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
title_sort Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
author Obregon, Dasiel
author_facet Obregon, Dasiel
Corona, Belkis G.
la Fuente, Jose de
Cabezas-Cruz, Alejandro
Goncalves, Luiz Ricardo [UNESP]
Matos, Carlos Antonio [UNESP]
Armas, Yasmani
Hinojosa, Yoandri
Alfonso, Pastor
Oliveira, Marcia C. S.
Machado, Rosangela Z. [UNESP]
author_role author
author2 Corona, Belkis G.
la Fuente, Jose de
Cabezas-Cruz, Alejandro
Goncalves, Luiz Ricardo [UNESP]
Matos, Carlos Antonio [UNESP]
Armas, Yasmani
Hinojosa, Yoandri
Alfonso, Pastor
Oliveira, Marcia C. S.
Machado, Rosangela Z. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Agr La Habana
Ctr Nacl Sanidad Agr
Inst Invest Recursos Cineget IREC CSIC UCLM JCCM
Oklahoma State Univ
Univ Paris Est
Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Obregon, Dasiel
Corona, Belkis G.
la Fuente, Jose de
Cabezas-Cruz, Alejandro
Goncalves, Luiz Ricardo [UNESP]
Matos, Carlos Antonio [UNESP]
Armas, Yasmani
Hinojosa, Yoandri
Alfonso, Pastor
Oliveira, Marcia C. S.
Machado, Rosangela Z. [UNESP]
dc.subject.por.fl_str_mv Water buffalo
Cattle
A. marginale
Rickettsemia
Genetic diversity
Cross-transmission
topic Water buffalo
Cattle
A. marginale
Rickettsemia
Genetic diversity
Cross-transmission
description Water buffalo (Bubalus bubalis) is a potential reservoir for Anaplasma marginale in livestock ecosystems of tropical countries. However, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. In the present study, the reservoir competence of water buffalo for A. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of A. marginale strains from cattle and buffalo. Eight groups of cattle and water buffaloes were randomly selected from cohabiting herds in four livestock ecosystems of Cuba, together with two control groups from unrelated cattle and buffalo herds. A total of 180 adult animals (88 water buffalo and 92 cattle) were sampled. Rickettsemia in carrier animals was determined by quantitative real-time PCR. The rickettsemia (parasitemia) levels in cattle were higher than in buffaloes, however the rickettsemia in buffalo may be enough to infect R. microplus ticks. The genetic diversity of A. marginale was assessed by strain characterization and phylogenetic analysis of 27 mspl a gene sequences. The results showed genetic similarity among strains from cattle and water buffalo, suggesting the occurrence of cross-species transmission.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-01
2021-06-25T21:07:58Z
2021-06-25T21:07:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vprsr.2018.06.007
Veterinary Parasitology- Regional Studies And Reports. Amsterdam: Elsevier, v. 13, p. 180-187, 2018.
2405-9390
http://hdl.handle.net/11449/210554
10.1016/j.vprsr.2018.06.007
WOS:000592468400034
url http://dx.doi.org/10.1016/j.vprsr.2018.06.007
http://hdl.handle.net/11449/210554
identifier_str_mv Veterinary Parasitology- Regional Studies And Reports. Amsterdam: Elsevier, v. 13, p. 180-187, 2018.
2405-9390
10.1016/j.vprsr.2018.06.007
WOS:000592468400034
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Parasitology- Regional Studies And Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 180-187
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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