Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vprsr.2018.06.007 http://hdl.handle.net/11449/210554 |
Resumo: | Water buffalo (Bubalus bubalis) is a potential reservoir for Anaplasma marginale in livestock ecosystems of tropical countries. However, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. In the present study, the reservoir competence of water buffalo for A. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of A. marginale strains from cattle and buffalo. Eight groups of cattle and water buffaloes were randomly selected from cohabiting herds in four livestock ecosystems of Cuba, together with two control groups from unrelated cattle and buffalo herds. A total of 180 adult animals (88 water buffalo and 92 cattle) were sampled. Rickettsemia in carrier animals was determined by quantitative real-time PCR. The rickettsemia (parasitemia) levels in cattle were higher than in buffaloes, however the rickettsemia in buffalo may be enough to infect R. microplus ticks. The genetic diversity of A. marginale was assessed by strain characterization and phylogenetic analysis of 27 mspl a gene sequences. The results showed genetic similarity among strains from cattle and water buffalo, suggesting the occurrence of cross-species transmission. |
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Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in CubaWater buffaloCattleA. marginaleRickettsemiaGenetic diversityCross-transmissionWater buffalo (Bubalus bubalis) is a potential reservoir for Anaplasma marginale in livestock ecosystems of tropical countries. However, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. In the present study, the reservoir competence of water buffalo for A. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of A. marginale strains from cattle and buffalo. Eight groups of cattle and water buffaloes were randomly selected from cohabiting herds in four livestock ecosystems of Cuba, together with two control groups from unrelated cattle and buffalo herds. A total of 180 adult animals (88 water buffalo and 92 cattle) were sampled. Rickettsemia in carrier animals was determined by quantitative real-time PCR. The rickettsemia (parasitemia) levels in cattle were higher than in buffaloes, however the rickettsemia in buffalo may be enough to infect R. microplus ticks. The genetic diversity of A. marginale was assessed by strain characterization and phylogenetic analysis of 27 mspl a gene sequences. The results showed genetic similarity among strains from cattle and water buffalo, suggesting the occurrence of cross-species transmission.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Priority Program for Animal and Plant Health, Ministry of Agriculture (MINAGRI), CubaConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Agr La Habana, Carretera Tapaste & Autopista Nacl, San Jose De Las Lajas 32700, Mayabeque, CubaCtr Nacl Sanidad Agr, Carretera Jamaica & Autopista Nacl, San Jose De Las Lajas 32700, Mayabeque, CubaInst Invest Recursos Cineget IREC CSIC UCLM JCCM, SaBio, Ciudad Real 13005, SpainOklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USAUniv Paris Est, ANSES, INRA, UMR BIPAR,Ecole Natl Vet Alfort, F-94700 Maisons Alfort, FranceUniv Estadual Paulista, Campus Jaboticabal, BR-14884900 Jaboticabal, SP, BrazilEmbrapa Pecuaria Sudeste, Rodovia Washington Luiz,Km 234, BR-13560970 Sao Carlos, SP, BrazilUniv Estadual Paulista, Campus Jaboticabal, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2012/21371-4National Priority Program for Animal and Plant Health, Ministry of Agriculture (MINAGRI), Cuba: P131LH003007CNPq: 302999/2013-2Elsevier B.V.Univ Agr La HabanaCtr Nacl Sanidad AgrInst Invest Recursos Cineget IREC CSIC UCLM JCCMOklahoma State UnivUniv Paris EstUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Obregon, DasielCorona, Belkis G.la Fuente, Jose deCabezas-Cruz, AlejandroGoncalves, Luiz Ricardo [UNESP]Matos, Carlos Antonio [UNESP]Armas, YasmaniHinojosa, YoandriAlfonso, PastorOliveira, Marcia C. S.Machado, Rosangela Z. [UNESP]2021-06-25T21:07:58Z2021-06-25T21:07:58Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article180-187http://dx.doi.org/10.1016/j.vprsr.2018.06.007Veterinary Parasitology- Regional Studies And Reports. Amsterdam: Elsevier, v. 13, p. 180-187, 2018.2405-9390http://hdl.handle.net/11449/21055410.1016/j.vprsr.2018.06.007WOS:000592468400034Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology- Regional Studies And Reportsinfo:eu-repo/semantics/openAccess2024-06-07T13:02:06Zoai:repositorio.unesp.br:11449/210554Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:35:03.679705Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba |
title |
Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba |
spellingShingle |
Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba Obregon, Dasiel Water buffalo Cattle A. marginale Rickettsemia Genetic diversity Cross-transmission |
title_short |
Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba |
title_full |
Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba |
title_fullStr |
Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba |
title_full_unstemmed |
Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba |
title_sort |
Molecular evidence of the reservoir competence of water buffalo (Bubalus bubalis) for Anaplasma marginale in Cuba |
author |
Obregon, Dasiel |
author_facet |
Obregon, Dasiel Corona, Belkis G. la Fuente, Jose de Cabezas-Cruz, Alejandro Goncalves, Luiz Ricardo [UNESP] Matos, Carlos Antonio [UNESP] Armas, Yasmani Hinojosa, Yoandri Alfonso, Pastor Oliveira, Marcia C. S. Machado, Rosangela Z. [UNESP] |
author_role |
author |
author2 |
Corona, Belkis G. la Fuente, Jose de Cabezas-Cruz, Alejandro Goncalves, Luiz Ricardo [UNESP] Matos, Carlos Antonio [UNESP] Armas, Yasmani Hinojosa, Yoandri Alfonso, Pastor Oliveira, Marcia C. S. Machado, Rosangela Z. [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Agr La Habana Ctr Nacl Sanidad Agr Inst Invest Recursos Cineget IREC CSIC UCLM JCCM Oklahoma State Univ Univ Paris Est Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Obregon, Dasiel Corona, Belkis G. la Fuente, Jose de Cabezas-Cruz, Alejandro Goncalves, Luiz Ricardo [UNESP] Matos, Carlos Antonio [UNESP] Armas, Yasmani Hinojosa, Yoandri Alfonso, Pastor Oliveira, Marcia C. S. Machado, Rosangela Z. [UNESP] |
dc.subject.por.fl_str_mv |
Water buffalo Cattle A. marginale Rickettsemia Genetic diversity Cross-transmission |
topic |
Water buffalo Cattle A. marginale Rickettsemia Genetic diversity Cross-transmission |
description |
Water buffalo (Bubalus bubalis) is a potential reservoir for Anaplasma marginale in livestock ecosystems of tropical countries. However, their participation in the epidemiological process of bovine anaplasmosis in endemic areas remains unclear. In the present study, the reservoir competence of water buffalo for A. marginale was explored by focusing on the analysis of rickettsemia levels in carrier animals, and the genetic characterization of A. marginale strains from cattle and buffalo. Eight groups of cattle and water buffaloes were randomly selected from cohabiting herds in four livestock ecosystems of Cuba, together with two control groups from unrelated cattle and buffalo herds. A total of 180 adult animals (88 water buffalo and 92 cattle) were sampled. Rickettsemia in carrier animals was determined by quantitative real-time PCR. The rickettsemia (parasitemia) levels in cattle were higher than in buffaloes, however the rickettsemia in buffalo may be enough to infect R. microplus ticks. The genetic diversity of A. marginale was assessed by strain characterization and phylogenetic analysis of 27 mspl a gene sequences. The results showed genetic similarity among strains from cattle and water buffalo, suggesting the occurrence of cross-species transmission. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-01 2021-06-25T21:07:58Z 2021-06-25T21:07:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vprsr.2018.06.007 Veterinary Parasitology- Regional Studies And Reports. Amsterdam: Elsevier, v. 13, p. 180-187, 2018. 2405-9390 http://hdl.handle.net/11449/210554 10.1016/j.vprsr.2018.06.007 WOS:000592468400034 |
url |
http://dx.doi.org/10.1016/j.vprsr.2018.06.007 http://hdl.handle.net/11449/210554 |
identifier_str_mv |
Veterinary Parasitology- Regional Studies And Reports. Amsterdam: Elsevier, v. 13, p. 180-187, 2018. 2405-9390 10.1016/j.vprsr.2018.06.007 WOS:000592468400034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Parasitology- Regional Studies And Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
180-187 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128829173006336 |