An easy way to detect dengue virus using nanoparticle-antibody conjugates

Detalhes bibliográficos
Autor(a) principal: Basso, Caroline R. [UNESP]
Data de Publicação: 2018
Outros Autores: Tozato, Claudia C. [UNESP], Crulhas, Bruno P. [UNESP], Castro, Gustavo R. [UNESP], Junior, João Pessoa A. [UNESP], Pedrosa, Valber A. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.virol.2017.10.001
http://hdl.handle.net/11449/175336
Resumo: The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID50 107 demonstrating a linear correlation between viral concentration and number of cells with an r2 value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits.
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spelling An easy way to detect dengue virus using nanoparticle-antibody conjugatesBiosensorFast detectionInfectious diagnosticsThe aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID50 107 demonstrating a linear correlation between viral concentration and number of cells with an r2 value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Chemistry and Biochemistry Institute of Bioscience UNESP-BotucatuDepartment of Microbiology and Immunology Institute of Bioscience UNESP-BotucatuDepartment of Chemistry and Biochemistry Institute of Bioscience UNESP-BotucatuDepartment of Microbiology and Immunology Institute of Bioscience UNESP-BotucatuFAPESP: 2016/159191-8CNPq: 304581/2016-0Universidade Estadual Paulista (Unesp)Basso, Caroline R. [UNESP]Tozato, Claudia C. [UNESP]Crulhas, Bruno P. [UNESP]Castro, Gustavo R. [UNESP]Junior, João Pessoa A. [UNESP]Pedrosa, Valber A. [UNESP]2018-12-11T17:15:21Z2018-12-11T17:15:21Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article85-90application/pdfhttp://dx.doi.org/10.1016/j.virol.2017.10.001Virology, v. 513, p. 85-90.1096-03410042-6822http://hdl.handle.net/11449/17533610.1016/j.virol.2017.10.0012-s2.0-850312946702-s2.0-85031294670.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirology1,728info:eu-repo/semantics/openAccess2023-10-14T06:03:40Zoai:repositorio.unesp.br:11449/175336Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:51:49.418287Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv An easy way to detect dengue virus using nanoparticle-antibody conjugates
title An easy way to detect dengue virus using nanoparticle-antibody conjugates
spellingShingle An easy way to detect dengue virus using nanoparticle-antibody conjugates
Basso, Caroline R. [UNESP]
Biosensor
Fast detection
Infectious diagnostics
title_short An easy way to detect dengue virus using nanoparticle-antibody conjugates
title_full An easy way to detect dengue virus using nanoparticle-antibody conjugates
title_fullStr An easy way to detect dengue virus using nanoparticle-antibody conjugates
title_full_unstemmed An easy way to detect dengue virus using nanoparticle-antibody conjugates
title_sort An easy way to detect dengue virus using nanoparticle-antibody conjugates
author Basso, Caroline R. [UNESP]
author_facet Basso, Caroline R. [UNESP]
Tozato, Claudia C. [UNESP]
Crulhas, Bruno P. [UNESP]
Castro, Gustavo R. [UNESP]
Junior, João Pessoa A. [UNESP]
Pedrosa, Valber A. [UNESP]
author_role author
author2 Tozato, Claudia C. [UNESP]
Crulhas, Bruno P. [UNESP]
Castro, Gustavo R. [UNESP]
Junior, João Pessoa A. [UNESP]
Pedrosa, Valber A. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Basso, Caroline R. [UNESP]
Tozato, Claudia C. [UNESP]
Crulhas, Bruno P. [UNESP]
Castro, Gustavo R. [UNESP]
Junior, João Pessoa A. [UNESP]
Pedrosa, Valber A. [UNESP]
dc.subject.por.fl_str_mv Biosensor
Fast detection
Infectious diagnostics
topic Biosensor
Fast detection
Infectious diagnostics
description The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID50 107 demonstrating a linear correlation between viral concentration and number of cells with an r2 value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:15:21Z
2018-12-11T17:15:21Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.virol.2017.10.001
Virology, v. 513, p. 85-90.
1096-0341
0042-6822
http://hdl.handle.net/11449/175336
10.1016/j.virol.2017.10.001
2-s2.0-85031294670
2-s2.0-85031294670.pdf
url http://dx.doi.org/10.1016/j.virol.2017.10.001
http://hdl.handle.net/11449/175336
identifier_str_mv Virology, v. 513, p. 85-90.
1096-0341
0042-6822
10.1016/j.virol.2017.10.001
2-s2.0-85031294670
2-s2.0-85031294670.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Virology
1,728
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 85-90
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128426617339904

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