Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.oooo.2012.02.002 http://hdl.handle.net/11449/22719 |
Resumo: | Objective. This study evaluated the effects of photodynamic therapy (PDT) on buccal candidiasis in mice and on the adherence of yeast to buccal epithelial cells (BECs) in vitro.Study Design. A total of 56 immunosuppressed mice with buccal candidiasis were subjected to PDT, consisting of treatment with erythrosine (400 mu mol/L) followed by exposure to a green LED (14.34 J cm(-2)). After treatment, the yeasts recovered from the mice were quantified (CFU/mL) and analyzed for the effects of PDT on their adherence to BECs. The data were analyzed using ANOVA, the Tukey test, Kruskal-Wallis test and Student t test.Results. PDT significantly reduced the amount of yeast present in the lesions by 0.73 log(10) (P = .018) and reduced C. albicans adherence to BECs by 35% without damaging adjacent tissues (P = .045).Conclusions. Photodynamic therapy exhibited antifungal effects against C. albicans biofilms formed in vivo and reduced the capacity of C. albicans to adhere to BECs in vitro. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114:67-74) |
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spelling |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cellsObjective. This study evaluated the effects of photodynamic therapy (PDT) on buccal candidiasis in mice and on the adherence of yeast to buccal epithelial cells (BECs) in vitro.Study Design. A total of 56 immunosuppressed mice with buccal candidiasis were subjected to PDT, consisting of treatment with erythrosine (400 mu mol/L) followed by exposure to a green LED (14.34 J cm(-2)). After treatment, the yeasts recovered from the mice were quantified (CFU/mL) and analyzed for the effects of PDT on their adherence to BECs. The data were analyzed using ANOVA, the Tukey test, Kruskal-Wallis test and Student t test.Results. PDT significantly reduced the amount of yeast present in the lesions by 0.73 log(10) (P = .018) and reduced C. albicans adherence to BECs by 35% without damaging adjacent tissues (P = .045).Conclusions. Photodynamic therapy exhibited antifungal effects against C. albicans biofilms formed in vivo and reduced the capacity of C. albicans to adhere to BECs in vitro. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114:67-74)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, Dept Biosci & Oral Diag, Sch Dent Sao Jose dos Campos, Sao Jose Dos Campos, SP, BrazilUNESP Univ Estadual Paulista, Dept Biosci & Oral Diag, Sch Dent Sao Jose dos Campos, Sao Jose Dos Campos, SP, BrazilFAPESP: 09/52048-1FAPESP: 10/18753-7FAPESP: 09/12005-1FAPESP: 10/01988-1FAPESP: 10/00879-4Elsevier B.V.Universidade Estadual Paulista (Unesp)Borges Pereira Costa, Anna Carolina [UNESP]Campos Rasteiro, Vanessa Maria [UNESP]Hashimoto, Emily Setsuko Halter da Silva [UNESP]Araujo, Cassia Fernandes [UNESP]Pereira, Cristiane Aparecida [UNESP]Junqueira, Juliana Campos [UNESP]Cardoso Jorge, Antonio Olavo [UNESP]2014-05-20T14:04:48Z2014-05-20T14:04:48Z2012-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article67-74application/pdfhttp://dx.doi.org/10.1016/j.oooo.2012.02.002Oral Surgery Oral Medicine Oral Pathology Oral Radiology. New York: Elsevier B.V., v. 114, n. 1, p. 67-74, 2012.2212-4403http://hdl.handle.net/11449/2271910.1016/j.oooo.2012.02.002WOS:000306286300025WOS000306286300025.pdf03220205410559000053567153623569Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOral Surgery Oral Medicine Oral Pathology Oral Radiology1.7180,720info:eu-repo/semantics/openAccess2023-11-06T06:13:58Zoai:repositorio.unesp.br:11449/22719Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:03:18.520766Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells |
title |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells |
spellingShingle |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells Borges Pereira Costa, Anna Carolina [UNESP] |
title_short |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells |
title_full |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells |
title_fullStr |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells |
title_full_unstemmed |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells |
title_sort |
Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells |
author |
Borges Pereira Costa, Anna Carolina [UNESP] |
author_facet |
Borges Pereira Costa, Anna Carolina [UNESP] Campos Rasteiro, Vanessa Maria [UNESP] Hashimoto, Emily Setsuko Halter da Silva [UNESP] Araujo, Cassia Fernandes [UNESP] Pereira, Cristiane Aparecida [UNESP] Junqueira, Juliana Campos [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] |
author_role |
author |
author2 |
Campos Rasteiro, Vanessa Maria [UNESP] Hashimoto, Emily Setsuko Halter da Silva [UNESP] Araujo, Cassia Fernandes [UNESP] Pereira, Cristiane Aparecida [UNESP] Junqueira, Juliana Campos [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Borges Pereira Costa, Anna Carolina [UNESP] Campos Rasteiro, Vanessa Maria [UNESP] Hashimoto, Emily Setsuko Halter da Silva [UNESP] Araujo, Cassia Fernandes [UNESP] Pereira, Cristiane Aparecida [UNESP] Junqueira, Juliana Campos [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] |
description |
Objective. This study evaluated the effects of photodynamic therapy (PDT) on buccal candidiasis in mice and on the adherence of yeast to buccal epithelial cells (BECs) in vitro.Study Design. A total of 56 immunosuppressed mice with buccal candidiasis were subjected to PDT, consisting of treatment with erythrosine (400 mu mol/L) followed by exposure to a green LED (14.34 J cm(-2)). After treatment, the yeasts recovered from the mice were quantified (CFU/mL) and analyzed for the effects of PDT on their adherence to BECs. The data were analyzed using ANOVA, the Tukey test, Kruskal-Wallis test and Student t test.Results. PDT significantly reduced the amount of yeast present in the lesions by 0.73 log(10) (P = .018) and reduced C. albicans adherence to BECs by 35% without damaging adjacent tissues (P = .045).Conclusions. Photodynamic therapy exhibited antifungal effects against C. albicans biofilms formed in vivo and reduced the capacity of C. albicans to adhere to BECs in vitro. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114:67-74) |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07-01 2014-05-20T14:04:48Z 2014-05-20T14:04:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.oooo.2012.02.002 Oral Surgery Oral Medicine Oral Pathology Oral Radiology. New York: Elsevier B.V., v. 114, n. 1, p. 67-74, 2012. 2212-4403 http://hdl.handle.net/11449/22719 10.1016/j.oooo.2012.02.002 WOS:000306286300025 WOS000306286300025.pdf 0322020541055900 0053567153623569 |
url |
http://dx.doi.org/10.1016/j.oooo.2012.02.002 http://hdl.handle.net/11449/22719 |
identifier_str_mv |
Oral Surgery Oral Medicine Oral Pathology Oral Radiology. New York: Elsevier B.V., v. 114, n. 1, p. 67-74, 2012. 2212-4403 10.1016/j.oooo.2012.02.002 WOS:000306286300025 WOS000306286300025.pdf 0322020541055900 0053567153623569 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oral Surgery Oral Medicine Oral Pathology Oral Radiology 1.718 0,720 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
67-74 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128745965355008 |