Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications

Detalhes bibliográficos
Autor(a) principal: Whelan, Simon Peter
Data de Publicação: 2022
Outros Autores: Tehrani, Zari, Peacock, Martin, Paulin, Joao Vitor [UNESP], Guy, Owen, Gethin, David
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jelechem.2021.115868
http://hdl.handle.net/11449/222971
Resumo: Screen printed pH sensors have been developed that utilise conductive carbon/graphene coated with DMSO-melanin for pH sensitivity. Their effectiveness has been demonstrated in buffer solutions and here they have been tested in a mixture of various frequently used culturing media solutions along side live bacteria in order to assess their suitability for monitoring the pH in real world bacterial culturing applications. The results indicate that these sensors can be used to accurately and consistently determine the pH of culturing media solutions at a high sensitivity in the presence of bacterial growth over the biologically relevant range of pH5 to pH8. This sensitivity was consistent between different types of culturing media that the sensor was tested with (brain heart infusion, nutrient and lysogeny broth) as well as pH reference buffer solutions. Therefore based on these findings it can be concluded that this sensor is sufficiently robust and has the potential to be useful in applications such as monitoring the ambient conditions of cultured microorganisms in a bioreactor.
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spelling Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applicationsBiosensorsGrapheneMicrobial culturepHScreen printed electrodeScreen printed pH sensors have been developed that utilise conductive carbon/graphene coated with DMSO-melanin for pH sensitivity. Their effectiveness has been demonstrated in buffer solutions and here they have been tested in a mixture of various frequently used culturing media solutions along side live bacteria in order to assess their suitability for monitoring the pH in real world bacterial culturing applications. The results indicate that these sensors can be used to accurately and consistently determine the pH of culturing media solutions at a high sensitivity in the presence of bacterial growth over the biologically relevant range of pH5 to pH8. This sensitivity was consistent between different types of culturing media that the sensor was tested with (brain heart infusion, nutrient and lysogeny broth) as well as pH reference buffer solutions. Therefore based on these findings it can be concluded that this sensor is sufficiently robust and has the potential to be useful in applications such as monitoring the ambient conditions of cultured microorganisms in a bioreactor.Welsh Centre for Printing and Coating College of Engineering Swansea UniversityCentre for Nanohealth Swansea University College of Engineering Swansea UniversitySao Paulo State University (UNESP) Department of Physics School of SciencesDepartment of Chemistry Swansea UniversityFuture Manufacturing Research Institute College of Engineering Swansea UniversityZimmer & Peacock AS – Bygg 24, Nedre vei 8Sao Paulo State University (UNESP) Department of Physics School of SciencesSwansea UniversityUniversidade Estadual Paulista (UNESP)Zimmer & Peacock AS – Bygg 24Whelan, Simon PeterTehrani, ZariPeacock, MartinPaulin, Joao Vitor [UNESP]Guy, OwenGethin, David2022-04-28T19:47:49Z2022-04-28T19:47:49Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jelechem.2021.115868Journal of Electroanalytical Chemistry, v. 904.1572-6657http://hdl.handle.net/11449/22297110.1016/j.jelechem.2021.1158682-s2.0-85120449800Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Electroanalytical Chemistryinfo:eu-repo/semantics/openAccess2022-04-28T19:47:49Zoai:repositorio.unesp.br:11449/222971Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:57:08.472671Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications
title Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications
spellingShingle Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications
Whelan, Simon Peter
Biosensors
Graphene
Microbial culture
pH
Screen printed electrode
title_short Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications
title_full Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications
title_fullStr Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications
title_full_unstemmed Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications
title_sort Investigation into the suitability of screen printed graphene-melanin pH sensors for use in bacterial culturing applications
author Whelan, Simon Peter
author_facet Whelan, Simon Peter
Tehrani, Zari
Peacock, Martin
Paulin, Joao Vitor [UNESP]
Guy, Owen
Gethin, David
author_role author
author2 Tehrani, Zari
Peacock, Martin
Paulin, Joao Vitor [UNESP]
Guy, Owen
Gethin, David
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Swansea University
Universidade Estadual Paulista (UNESP)
Zimmer & Peacock AS – Bygg 24
dc.contributor.author.fl_str_mv Whelan, Simon Peter
Tehrani, Zari
Peacock, Martin
Paulin, Joao Vitor [UNESP]
Guy, Owen
Gethin, David
dc.subject.por.fl_str_mv Biosensors
Graphene
Microbial culture
pH
Screen printed electrode
topic Biosensors
Graphene
Microbial culture
pH
Screen printed electrode
description Screen printed pH sensors have been developed that utilise conductive carbon/graphene coated with DMSO-melanin for pH sensitivity. Their effectiveness has been demonstrated in buffer solutions and here they have been tested in a mixture of various frequently used culturing media solutions along side live bacteria in order to assess their suitability for monitoring the pH in real world bacterial culturing applications. The results indicate that these sensors can be used to accurately and consistently determine the pH of culturing media solutions at a high sensitivity in the presence of bacterial growth over the biologically relevant range of pH5 to pH8. This sensitivity was consistent between different types of culturing media that the sensor was tested with (brain heart infusion, nutrient and lysogeny broth) as well as pH reference buffer solutions. Therefore based on these findings it can be concluded that this sensor is sufficiently robust and has the potential to be useful in applications such as monitoring the ambient conditions of cultured microorganisms in a bioreactor.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T19:47:49Z
2022-04-28T19:47:49Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jelechem.2021.115868
Journal of Electroanalytical Chemistry, v. 904.
1572-6657
http://hdl.handle.net/11449/222971
10.1016/j.jelechem.2021.115868
2-s2.0-85120449800
url http://dx.doi.org/10.1016/j.jelechem.2021.115868
http://hdl.handle.net/11449/222971
identifier_str_mv Journal of Electroanalytical Chemistry, v. 904.
1572-6657
10.1016/j.jelechem.2021.115868
2-s2.0-85120449800
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Electroanalytical Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129266423955456