Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-020-03624-7 http://hdl.handle.net/11449/208042 |
Resumo: | Objectives: the objective of the present exploratory study was to determine bacterial diversity and endotoxin levels in deep carious lesions of teeth presenting symptoms of reversible pulpitis. Materials and methods: Twenty patients with deep carious lesions, reporting clinical symptomatology compatible with reversible pulpitis (n = 10) or not reporting clinical symptomatology (n = 10), were selected. Carious dentin samples were obtained with the aid of sterile and pyrogen-free spoon excavators and harvested in two steps: before and after infected dentin removal. Samples were collected for checkerboard and for kinetic chromogenic LAL assay for determination of microbial profile and quantitation of endotoxin, respectively. Data were analyzed by Mann Whitney for bacteria and two-way ANOVA for endotoxins (5%). Results: No difference on the studied bacteria was detected between the superficial and deep dentin layers. Symptomatic teeth showed greater presence of Lactobacillus species, Capnocytophaga sputigena, and Leptotrichia buccalis. For the endotoxins, symptomatic teeth resulted in greater quantity of endotoxins (p = 0.047), being 4.13 log10 EU/mL/μg dentin and 3.45 log10 EU/mL/μg dentin, for symptomatic and asymptomatic teeth, respectively. Dentin collected in different areas presented similar number of endotoxins (p = 0.139). Conclusion: The amount of the studied bacteria does not seem to be related to reported symptomatology of deep carious lesions, while endotoxins quantity is greater in symptomatic scenarios, regardless of the harvesting area. Clinical relevance: The understanding of bacterial amount in reversible pulpitis is important to establish a clinical protocol of treatment. |
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Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenariosbacteriadental cariesdentinendotoxinpulpitisObjectives: the objective of the present exploratory study was to determine bacterial diversity and endotoxin levels in deep carious lesions of teeth presenting symptoms of reversible pulpitis. Materials and methods: Twenty patients with deep carious lesions, reporting clinical symptomatology compatible with reversible pulpitis (n = 10) or not reporting clinical symptomatology (n = 10), were selected. Carious dentin samples were obtained with the aid of sterile and pyrogen-free spoon excavators and harvested in two steps: before and after infected dentin removal. Samples were collected for checkerboard and for kinetic chromogenic LAL assay for determination of microbial profile and quantitation of endotoxin, respectively. Data were analyzed by Mann Whitney for bacteria and two-way ANOVA for endotoxins (5%). Results: No difference on the studied bacteria was detected between the superficial and deep dentin layers. Symptomatic teeth showed greater presence of Lactobacillus species, Capnocytophaga sputigena, and Leptotrichia buccalis. For the endotoxins, symptomatic teeth resulted in greater quantity of endotoxins (p = 0.047), being 4.13 log10 EU/mL/μg dentin and 3.45 log10 EU/mL/μg dentin, for symptomatic and asymptomatic teeth, respectively. Dentin collected in different areas presented similar number of endotoxins (p = 0.139). Conclusion: The amount of the studied bacteria does not seem to be related to reported symptomatology of deep carious lesions, while endotoxins quantity is greater in symptomatic scenarios, regardless of the harvesting area. Clinical relevance: The understanding of bacterial amount in reversible pulpitis is important to establish a clinical protocol of treatment.Department of Restorative Dentistry Institute of Science and Technology São Paulo State University - UNESPDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESPDepartment of Restorative Dentistry Institute of Science and Technology São Paulo State University - UNESPDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University - UNESPUniversidade Estadual Paulista (Unesp)Caneppele, Taciana Marco Ferraz [UNESP]de Souza, Letícia Grilo [UNESP]Spinola, Manuela da Silva [UNESP]de Oliveira, Felipe Eduardo [UNESP]de Oliveira, Luciane Dias [UNESP]Carvalho, Cláudio Antônio Talge [UNESP]Bresciani, Eduardo [UNESP]2021-06-25T11:05:21Z2021-06-25T11:05:21Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3033-3042http://dx.doi.org/10.1007/s00784-020-03624-7Clinical Oral Investigations, v. 25, n. 5, p. 3033-3042, 2021.1436-37711432-6981http://hdl.handle.net/11449/20804210.1007/s00784-020-03624-72-s2.0-8509258297293046001665831000000-0003-0987-5594Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2021-12-13T15:15:46Zoai:repositorio.unesp.br:11449/208042Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-12-13T15:15:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios |
title |
Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios |
spellingShingle |
Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios Caneppele, Taciana Marco Ferraz [UNESP] bacteria dental caries dentin endotoxin pulpitis |
title_short |
Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios |
title_full |
Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios |
title_fullStr |
Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios |
title_full_unstemmed |
Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios |
title_sort |
Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios |
author |
Caneppele, Taciana Marco Ferraz [UNESP] |
author_facet |
Caneppele, Taciana Marco Ferraz [UNESP] de Souza, Letícia Grilo [UNESP] Spinola, Manuela da Silva [UNESP] de Oliveira, Felipe Eduardo [UNESP] de Oliveira, Luciane Dias [UNESP] Carvalho, Cláudio Antônio Talge [UNESP] Bresciani, Eduardo [UNESP] |
author_role |
author |
author2 |
de Souza, Letícia Grilo [UNESP] Spinola, Manuela da Silva [UNESP] de Oliveira, Felipe Eduardo [UNESP] de Oliveira, Luciane Dias [UNESP] Carvalho, Cláudio Antônio Talge [UNESP] Bresciani, Eduardo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Caneppele, Taciana Marco Ferraz [UNESP] de Souza, Letícia Grilo [UNESP] Spinola, Manuela da Silva [UNESP] de Oliveira, Felipe Eduardo [UNESP] de Oliveira, Luciane Dias [UNESP] Carvalho, Cláudio Antônio Talge [UNESP] Bresciani, Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
bacteria dental caries dentin endotoxin pulpitis |
topic |
bacteria dental caries dentin endotoxin pulpitis |
description |
Objectives: the objective of the present exploratory study was to determine bacterial diversity and endotoxin levels in deep carious lesions of teeth presenting symptoms of reversible pulpitis. Materials and methods: Twenty patients with deep carious lesions, reporting clinical symptomatology compatible with reversible pulpitis (n = 10) or not reporting clinical symptomatology (n = 10), were selected. Carious dentin samples were obtained with the aid of sterile and pyrogen-free spoon excavators and harvested in two steps: before and after infected dentin removal. Samples were collected for checkerboard and for kinetic chromogenic LAL assay for determination of microbial profile and quantitation of endotoxin, respectively. Data were analyzed by Mann Whitney for bacteria and two-way ANOVA for endotoxins (5%). Results: No difference on the studied bacteria was detected between the superficial and deep dentin layers. Symptomatic teeth showed greater presence of Lactobacillus species, Capnocytophaga sputigena, and Leptotrichia buccalis. For the endotoxins, symptomatic teeth resulted in greater quantity of endotoxins (p = 0.047), being 4.13 log10 EU/mL/μg dentin and 3.45 log10 EU/mL/μg dentin, for symptomatic and asymptomatic teeth, respectively. Dentin collected in different areas presented similar number of endotoxins (p = 0.139). Conclusion: The amount of the studied bacteria does not seem to be related to reported symptomatology of deep carious lesions, while endotoxins quantity is greater in symptomatic scenarios, regardless of the harvesting area. Clinical relevance: The understanding of bacterial amount in reversible pulpitis is important to establish a clinical protocol of treatment. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:05:21Z 2021-06-25T11:05:21Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-020-03624-7 Clinical Oral Investigations, v. 25, n. 5, p. 3033-3042, 2021. 1436-3771 1432-6981 http://hdl.handle.net/11449/208042 10.1007/s00784-020-03624-7 2-s2.0-85092582972 9304600166583100 0000-0003-0987-5594 |
url |
http://dx.doi.org/10.1007/s00784-020-03624-7 http://hdl.handle.net/11449/208042 |
identifier_str_mv |
Clinical Oral Investigations, v. 25, n. 5, p. 3033-3042, 2021. 1436-3771 1432-6981 10.1007/s00784-020-03624-7 2-s2.0-85092582972 9304600166583100 0000-0003-0987-5594 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3033-3042 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964441331105792 |