Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1089/zeb.2016.1380 http://hdl.handle.net/11449/162854 |
Resumo: | The mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconamericus, with diploid number preserved, but with different karyotype formulae. Bryconamericus ecai, from the Forquetinha river/RS, presented three new cytotypes, increasing the number of karyotype forms to seven in this population. Other two populations of Bryconamericus sp. from the Vermelho stream/PR and Cambuta river/PR exhibited interpopulation variation. The chromosome mapping of rDNA sites revealed unique markings among the three populations, showing inter-and intrapopulation variability located in the terminal region. The molecular analysis using DNA barcoding complementing the cytogenetic analysis also showed differentiation among the three populations. The U2 small nuclear DNA repetitive sequence exhibited conserved features, being located in the interstitial region of a single chromosome pair. This is the first report on its occurrence in the genus Bryconamericus. Data obtained revealed a karyotype variability already assigned to the genus, along with polymorphism of ribosomal sites, demonstrating that this group of fish can be undergoing a divergent evolutionary process, constituting a substantive model for studies of chromosomal evolution. |
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Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populationsfish cytogeneticsmolecular cytogeneticsribosomal DNAU2 snDNAkaryotypic polymorphismThe mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconamericus, with diploid number preserved, but with different karyotype formulae. Bryconamericus ecai, from the Forquetinha river/RS, presented three new cytotypes, increasing the number of karyotype forms to seven in this population. Other two populations of Bryconamericus sp. from the Vermelho stream/PR and Cambuta river/PR exhibited interpopulation variation. The chromosome mapping of rDNA sites revealed unique markings among the three populations, showing inter-and intrapopulation variability located in the terminal region. The molecular analysis using DNA barcoding complementing the cytogenetic analysis also showed differentiation among the three populations. The U2 small nuclear DNA repetitive sequence exhibited conserved features, being located in the interstitial region of a single chromosome pair. This is the first report on its occurrence in the genus Bryconamericus. Data obtained revealed a karyotype variability already assigned to the genus, along with polymorphism of ribosomal sites, demonstrating that this group of fish can be undergoing a divergent evolutionary process, constituting a substantive model for studies of chromosomal evolution.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Londrina, Dept Biol Geral, Lab Citogenet Anim, Rodovia Celso Garcia Cida, BR-86057970 Londrina, BrazilUniv Chile, ICBM Fac Med, Lab Citogenet Vertebrados, Santiago, ChileUniv Estadual Londrina, Dept Biol Geral, Lab Genet & Ecol Anim, Londrina, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, BrazilMary Ann Liebert, IncUniversidade Estadual de Londrina (UEL)Univ ChileUniversidade Estadual Paulista (Unesp)Santos, Angelica Rossotti dosUsso, Mariana CampanerGouveia, Juceli GonzalezAraya-Jaime, Cristian [UNESP]Frantine-Silva, WilsonGiuliano-Caetano, LuciaForesti, Fausto [UNESP]Dias, Ana Lucia2018-11-26T17:34:41Z2018-11-26T17:34:41Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article261-271http://dx.doi.org/10.1089/zeb.2016.1380Zebrafish. New Rochelle: Mary Ann Liebert, Inc, v. 14, n. 3, p. 261-271, 2017.1545-8547http://hdl.handle.net/11449/16285410.1089/zeb.2016.1380WOS:000402566000008Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZebrafish0,795info:eu-repo/semantics/openAccess2021-10-23T16:30:36Zoai:repositorio.unesp.br:11449/162854Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:45:07.282395Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations |
title |
Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations |
spellingShingle |
Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations Santos, Angelica Rossotti dos fish cytogenetics molecular cytogenetics ribosomal DNA U2 snDNA karyotypic polymorphism |
title_short |
Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations |
title_full |
Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations |
title_fullStr |
Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations |
title_full_unstemmed |
Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations |
title_sort |
Chromosomal Mapping of Repetitive DNA Sequences in the Genus Bryconamericus (Characidae) and DNA Barcoding to Differentiate Populations |
author |
Santos, Angelica Rossotti dos |
author_facet |
Santos, Angelica Rossotti dos Usso, Mariana Campaner Gouveia, Juceli Gonzalez Araya-Jaime, Cristian [UNESP] Frantine-Silva, Wilson Giuliano-Caetano, Lucia Foresti, Fausto [UNESP] Dias, Ana Lucia |
author_role |
author |
author2 |
Usso, Mariana Campaner Gouveia, Juceli Gonzalez Araya-Jaime, Cristian [UNESP] Frantine-Silva, Wilson Giuliano-Caetano, Lucia Foresti, Fausto [UNESP] Dias, Ana Lucia |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Univ Chile Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santos, Angelica Rossotti dos Usso, Mariana Campaner Gouveia, Juceli Gonzalez Araya-Jaime, Cristian [UNESP] Frantine-Silva, Wilson Giuliano-Caetano, Lucia Foresti, Fausto [UNESP] Dias, Ana Lucia |
dc.subject.por.fl_str_mv |
fish cytogenetics molecular cytogenetics ribosomal DNA U2 snDNA karyotypic polymorphism |
topic |
fish cytogenetics molecular cytogenetics ribosomal DNA U2 snDNA karyotypic polymorphism |
description |
The mapping of repetitive DNA sites by fluorescence in situ hybridization has been widely used for karyotype studies in different species of fish, especially when dealing with related species or even genera presenting high chromosome variability. This study analyzed three populations of Bryconamericus, with diploid number preserved, but with different karyotype formulae. Bryconamericus ecai, from the Forquetinha river/RS, presented three new cytotypes, increasing the number of karyotype forms to seven in this population. Other two populations of Bryconamericus sp. from the Vermelho stream/PR and Cambuta river/PR exhibited interpopulation variation. The chromosome mapping of rDNA sites revealed unique markings among the three populations, showing inter-and intrapopulation variability located in the terminal region. The molecular analysis using DNA barcoding complementing the cytogenetic analysis also showed differentiation among the three populations. The U2 small nuclear DNA repetitive sequence exhibited conserved features, being located in the interstitial region of a single chromosome pair. This is the first report on its occurrence in the genus Bryconamericus. Data obtained revealed a karyotype variability already assigned to the genus, along with polymorphism of ribosomal sites, demonstrating that this group of fish can be undergoing a divergent evolutionary process, constituting a substantive model for studies of chromosomal evolution. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2018-11-26T17:34:41Z 2018-11-26T17:34:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1089/zeb.2016.1380 Zebrafish. New Rochelle: Mary Ann Liebert, Inc, v. 14, n. 3, p. 261-271, 2017. 1545-8547 http://hdl.handle.net/11449/162854 10.1089/zeb.2016.1380 WOS:000402566000008 |
url |
http://dx.doi.org/10.1089/zeb.2016.1380 http://hdl.handle.net/11449/162854 |
identifier_str_mv |
Zebrafish. New Rochelle: Mary Ann Liebert, Inc, v. 14, n. 3, p. 261-271, 2017. 1545-8547 10.1089/zeb.2016.1380 WOS:000402566000008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zebrafish 0,795 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
261-271 |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert, Inc |
publisher.none.fl_str_mv |
Mary Ann Liebert, Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129242219675648 |