MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE)
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.ncbi.nlm.nih.gov/pubmed/1307406 http://hdl.handle.net/11449/33467 |
Resumo: | Calomys callosus a wild rodent, previously described as harboring Trypanosoma cruzi, has a low susceptibility to infection by this protozoan.Experiments were designed to evaluate the contribution of the immune response to the resistance to T. cruzi infection exhibited by C. callosus. Animals were submitted to injections of high (200 mg/kg body weight) and low (20 mg/kg body weight) doses of cyclophosphamide on days -1 or -1 and +5, and inoculated with 4 x 10(3) T. cruzi on day O. Parasitemia, mortality and antibody response as measured by direct agglutination of trypomastigotes were observed. Two hundred mg doses of cyclophosphamide resulted in higher parasitemia and mortality as well as in suppression of the antibody response. A single dose of 20 mg enhanced antibody levels on the 20th day after infection, while an additional dose did not further increase antibody production. Parasitemia levels were not depressed, but rather increased in both these groups as compared to untreated controls. Passive transfer of hyperimmune C. callosus anti-T. cruzi serum to cyclophosphamide immunosuppressed animals resulted in lower parasitemia and mortality rates. These results indicate that the immune response plays an important role in the resistance of C. callosus to T. cruzi. |
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MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE)CALOMYS-CALLOSUSCYCLOPHOSPHAMIDETRYPANOSOMA-CRUZIPARASITEMIAMORTALITYANTIBODY RESPONSECalomys callosus a wild rodent, previously described as harboring Trypanosoma cruzi, has a low susceptibility to infection by this protozoan.Experiments were designed to evaluate the contribution of the immune response to the resistance to T. cruzi infection exhibited by C. callosus. Animals were submitted to injections of high (200 mg/kg body weight) and low (20 mg/kg body weight) doses of cyclophosphamide on days -1 or -1 and +5, and inoculated with 4 x 10(3) T. cruzi on day O. Parasitemia, mortality and antibody response as measured by direct agglutination of trypomastigotes were observed. Two hundred mg doses of cyclophosphamide resulted in higher parasitemia and mortality as well as in suppression of the antibody response. A single dose of 20 mg enhanced antibody levels on the 20th day after infection, while an additional dose did not further increase antibody production. Parasitemia levels were not depressed, but rather increased in both these groups as compared to untreated controls. Passive transfer of hyperimmune C. callosus anti-T. cruzi serum to cyclophosphamide immunosuppressed animals resulted in lower parasitemia and mortality rates. These results indicate that the immune response plays an important role in the resistance of C. callosus to T. cruzi.INST MED TROP SAO PAULO,SAO PAULO,BRAZILUNIV ESTADUAL PAULISTA,INST BASICO BIOL MED & AGR,DEPT STAT,BOTUCATU,SP,BRAZILUNIV SAO PAULO,INST CIENCIAS BIOMED,DEPT PARASITOL,SAO PAULO,BRAZILUNIV ESTADUAL PAULISTA,INST BASICO BIOL MED & AGR,DEPT STAT,BOTUCATU,SP,BRAZILInst Medicina TropicalUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Borges, M. M.Curi, P. R.Kloetzel, J. K.2014-05-20T15:22:30Z2014-05-20T15:22:30Z1992-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8http://www.ncbi.nlm.nih.gov/pubmed/1307406Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical, v. 34, n. 1, p. 1-8, 1992.0036-4665http://hdl.handle.net/11449/33467WOS:A1992HU08200001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Instituto de Medicina Tropical de São Paulo1.4890,669info:eu-repo/semantics/openAccess2021-10-22T22:17:01Zoai:repositorio.unesp.br:11449/33467Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:56:22.455687Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE) |
title |
MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE) |
spellingShingle |
MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE) Borges, M. M. CALOMYS-CALLOSUS CYCLOPHOSPHAMIDE TRYPANOSOMA-CRUZI PARASITEMIA MORTALITY ANTIBODY RESPONSE |
title_short |
MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE) |
title_full |
MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE) |
title_fullStr |
MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE) |
title_full_unstemmed |
MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE) |
title_sort |
MODULATION OF PARASITEMIA AND ANTIBODY-RESPONSE TO TRYPANOSOMA-CRUZI BY CYCLOPHOSPHAMIDE IN CALOMYS-CALLOSUS (RODENTIA, CRICETIDAE) |
author |
Borges, M. M. |
author_facet |
Borges, M. M. Curi, P. R. Kloetzel, J. K. |
author_role |
author |
author2 |
Curi, P. R. Kloetzel, J. K. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Borges, M. M. Curi, P. R. Kloetzel, J. K. |
dc.subject.por.fl_str_mv |
CALOMYS-CALLOSUS CYCLOPHOSPHAMIDE TRYPANOSOMA-CRUZI PARASITEMIA MORTALITY ANTIBODY RESPONSE |
topic |
CALOMYS-CALLOSUS CYCLOPHOSPHAMIDE TRYPANOSOMA-CRUZI PARASITEMIA MORTALITY ANTIBODY RESPONSE |
description |
Calomys callosus a wild rodent, previously described as harboring Trypanosoma cruzi, has a low susceptibility to infection by this protozoan.Experiments were designed to evaluate the contribution of the immune response to the resistance to T. cruzi infection exhibited by C. callosus. Animals were submitted to injections of high (200 mg/kg body weight) and low (20 mg/kg body weight) doses of cyclophosphamide on days -1 or -1 and +5, and inoculated with 4 x 10(3) T. cruzi on day O. Parasitemia, mortality and antibody response as measured by direct agglutination of trypomastigotes were observed. Two hundred mg doses of cyclophosphamide resulted in higher parasitemia and mortality as well as in suppression of the antibody response. A single dose of 20 mg enhanced antibody levels on the 20th day after infection, while an additional dose did not further increase antibody production. Parasitemia levels were not depressed, but rather increased in both these groups as compared to untreated controls. Passive transfer of hyperimmune C. callosus anti-T. cruzi serum to cyclophosphamide immunosuppressed animals resulted in lower parasitemia and mortality rates. These results indicate that the immune response plays an important role in the resistance of C. callosus to T. cruzi. |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-01-01 2014-05-20T15:22:30Z 2014-05-20T15:22:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.ncbi.nlm.nih.gov/pubmed/1307406 Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical, v. 34, n. 1, p. 1-8, 1992. 0036-4665 http://hdl.handle.net/11449/33467 WOS:A1992HU08200001 |
url |
http://www.ncbi.nlm.nih.gov/pubmed/1307406 http://hdl.handle.net/11449/33467 |
identifier_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo. São Paulo: Inst Medicina Tropical, v. 34, n. 1, p. 1-8, 1992. 0036-4665 WOS:A1992HU08200001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo 1.489 0,669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 |
dc.publisher.none.fl_str_mv |
Inst Medicina Tropical |
publisher.none.fl_str_mv |
Inst Medicina Tropical |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129476430659584 |