RAPD marker use for improving resistance to Helicoverpa zea in corn

Detalhes bibliográficos
Autor(a) principal: Da Silva, S. C. [UNESP]
Data de Publicação: 2000
Outros Autores: Lemos, M. V.F. [UNESP], Ayala Osuna, J. T. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/224178
Resumo: Helicoverpa zea is responsible for great losses to the corn, Zea mays L., crops final productivity, and the best way to control it is by improving genetic resistance. In collaboration with corn improvement and increasing resistance to insects through molecular marker assisted selection, this work had as an objective the selection of resistant (RP) and susceptible progenies (SP) to H. zea based on the RAPD technique. Molecular markers were found, among the resistant progenies and it is suggested that linkage of these within the Zapalote Chico corn race, be used to extract resistance genes from this race as a donor. The progenies were selected from a population of half-sibs exhibiting a broader genetic base (FCAVJ-VFJ4). After DNA extraction, two sample bulks were formed; one made up of the six most resistant plants, the other of the six least resistant plants. Eighty-six primers were tested for PCR reactions with the resistant and susceptible bulks and analyzed on agarose electrophoresis for the detection of RAPD band polymorphism. The results of the banding patterns and similarity values indicated a nucleotide sequence amplified by the primer OPC-2 as a possible molecular marker for the identification of resistant progenies and a homology region between them and the Zapalote Chico corn race.
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spelling RAPD marker use for improving resistance to Helicoverpa zea in cornMarker-assisted selectionRAPDZea maysHelicoverpa zea is responsible for great losses to the corn, Zea mays L., crops final productivity, and the best way to control it is by improving genetic resistance. In collaboration with corn improvement and increasing resistance to insects through molecular marker assisted selection, this work had as an objective the selection of resistant (RP) and susceptible progenies (SP) to H. zea based on the RAPD technique. Molecular markers were found, among the resistant progenies and it is suggested that linkage of these within the Zapalote Chico corn race, be used to extract resistance genes from this race as a donor. The progenies were selected from a population of half-sibs exhibiting a broader genetic base (FCAVJ-VFJ4). After DNA extraction, two sample bulks were formed; one made up of the six most resistant plants, the other of the six least resistant plants. Eighty-six primers were tested for PCR reactions with the resistant and susceptible bulks and analyzed on agarose electrophoresis for the detection of RAPD band polymorphism. The results of the banding patterns and similarity values indicated a nucleotide sequence amplified by the primer OPC-2 as a possible molecular marker for the identification of resistant progenies and a homology region between them and the Zapalote Chico corn race.Depto. Biol. Aplicada a Agropecuaria UNESP, Via Acesso Prof. P. D. Castellane, CEP 14884-900 Jaboticabal (SP)Depto. Biol. Aplicada a Agropecuaria UNESP, Via Acesso Prof. P. D. Castellane, CEP 14884-900 Jaboticabal (SP)Universidade Estadual Paulista (UNESP)Da Silva, S. C. [UNESP]Lemos, M. V.F. [UNESP]Ayala Osuna, J. T. [UNESP]2022-04-28T19:55:02Z2022-04-28T19:55:02Z2000-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article289-294Maydica, v. 45, n. 4, p. 289-294, 2000.0025-6153http://hdl.handle.net/11449/2241782-s2.0-0034468444Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaydicainfo:eu-repo/semantics/openAccess2024-06-06T13:04:45Zoai:repositorio.unesp.br:11449/224178Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:58:07.088510Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv RAPD marker use for improving resistance to Helicoverpa zea in corn
title RAPD marker use for improving resistance to Helicoverpa zea in corn
spellingShingle RAPD marker use for improving resistance to Helicoverpa zea in corn
Da Silva, S. C. [UNESP]
Marker-assisted selection
RAPD
Zea mays
title_short RAPD marker use for improving resistance to Helicoverpa zea in corn
title_full RAPD marker use for improving resistance to Helicoverpa zea in corn
title_fullStr RAPD marker use for improving resistance to Helicoverpa zea in corn
title_full_unstemmed RAPD marker use for improving resistance to Helicoverpa zea in corn
title_sort RAPD marker use for improving resistance to Helicoverpa zea in corn
author Da Silva, S. C. [UNESP]
author_facet Da Silva, S. C. [UNESP]
Lemos, M. V.F. [UNESP]
Ayala Osuna, J. T. [UNESP]
author_role author
author2 Lemos, M. V.F. [UNESP]
Ayala Osuna, J. T. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Da Silva, S. C. [UNESP]
Lemos, M. V.F. [UNESP]
Ayala Osuna, J. T. [UNESP]
dc.subject.por.fl_str_mv Marker-assisted selection
RAPD
Zea mays
topic Marker-assisted selection
RAPD
Zea mays
description Helicoverpa zea is responsible for great losses to the corn, Zea mays L., crops final productivity, and the best way to control it is by improving genetic resistance. In collaboration with corn improvement and increasing resistance to insects through molecular marker assisted selection, this work had as an objective the selection of resistant (RP) and susceptible progenies (SP) to H. zea based on the RAPD technique. Molecular markers were found, among the resistant progenies and it is suggested that linkage of these within the Zapalote Chico corn race, be used to extract resistance genes from this race as a donor. The progenies were selected from a population of half-sibs exhibiting a broader genetic base (FCAVJ-VFJ4). After DNA extraction, two sample bulks were formed; one made up of the six most resistant plants, the other of the six least resistant plants. Eighty-six primers were tested for PCR reactions with the resistant and susceptible bulks and analyzed on agarose electrophoresis for the detection of RAPD band polymorphism. The results of the banding patterns and similarity values indicated a nucleotide sequence amplified by the primer OPC-2 as a possible molecular marker for the identification of resistant progenies and a homology region between them and the Zapalote Chico corn race.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
2022-04-28T19:55:02Z
2022-04-28T19:55:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Maydica, v. 45, n. 4, p. 289-294, 2000.
0025-6153
http://hdl.handle.net/11449/224178
2-s2.0-0034468444
identifier_str_mv Maydica, v. 45, n. 4, p. 289-294, 2000.
0025-6153
2-s2.0-0034468444
url http://hdl.handle.net/11449/224178
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Maydica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 289-294
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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