Bacterial functional redundancy along a soil reclamation gradient
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.1128/AEM.66.10.4361-4365.2000 |
Texto Completo: | http://dx.doi.org/10.1128/AEM.66.10.4361-4365.2000 http://hdl.handle.net/11449/3957 |
Resumo: | A strategy to measure bacterial functional redundancy was developed and tested with soils collected along a soil reclamation gradient by determining the richness and diversity of bacterial groups capable of in situ growth on selected carbon substrates. Soil cores were collected from four sites along a transect from the Jamari tin mine site in the Jamari National Forest, Rondonia, RO, Brazil: denuded mine spoil, soil from below the canopy of invading pioneer trees, revegetated soil under new growth on the forest edge, and the forest floor of an adjacent preserved forest. Bacterial population responses were analyzed by amending these soil samples with individual carbon substrates in the presence of bromodeoxyuridine (BrdU), BrdU-labeled DNA was then subjected to a 16S-23S rRNA intergenic analysis to depict the actively growing bacteria from each site, the number and diversity of bacterial groups responding to four carbon substrates (L-serine, L-threonine, sodium citrate, and or-lactose hydrate) increased along the reclamation-vegetation gradient such that the preserved forest soil samples contained the highest functional redundancy for each substrate. These data suggest that bacterial functional redundancy increases in relation to the regrowth of plant communities and may therefore represent an important aspect of the restoration of soil biological functionality to reclaimed mine spoils. They also suggest that bacterial functional redundancy may be a useful indicator of soil quality and ecosystem functioning. |
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Bacterial functional redundancy along a soil reclamation gradientA strategy to measure bacterial functional redundancy was developed and tested with soils collected along a soil reclamation gradient by determining the richness and diversity of bacterial groups capable of in situ growth on selected carbon substrates. Soil cores were collected from four sites along a transect from the Jamari tin mine site in the Jamari National Forest, Rondonia, RO, Brazil: denuded mine spoil, soil from below the canopy of invading pioneer trees, revegetated soil under new growth on the forest edge, and the forest floor of an adjacent preserved forest. Bacterial population responses were analyzed by amending these soil samples with individual carbon substrates in the presence of bromodeoxyuridine (BrdU), BrdU-labeled DNA was then subjected to a 16S-23S rRNA intergenic analysis to depict the actively growing bacteria from each site, the number and diversity of bacterial groups responding to four carbon substrates (L-serine, L-threonine, sodium citrate, and or-lactose hydrate) increased along the reclamation-vegetation gradient such that the preserved forest soil samples contained the highest functional redundancy for each substrate. These data suggest that bacterial functional redundancy increases in relation to the regrowth of plant communities and may therefore represent an important aspect of the restoration of soil biological functionality to reclaimed mine spoils. They also suggest that bacterial functional redundancy may be a useful indicator of soil quality and ecosystem functioning.Univ Calif Riverside, Dept Plant Pathol, Riverside, CA 92521 USAUniv Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USAUniv São Paulo, ESALQ, Dept Soil Sci, BR-13418900 Piracicaba, BrazilUniv Estadual Paulista, Dept Technol, BR-14870000 Jaboticabal, SP, BrazilUniv Estadual Paulista, Dept Technol, BR-14870000 Jaboticabal, SP, BrazilAmer Soc MicrobiologyUniversity of California, Riverside (UCR)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Yin, B.Crowley, D.Sparovek, G.De Melo, W. J.Borneman, J.2014-05-20T13:17:31Z2014-05-20T13:17:31Z2000-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4361-4365application/pdfhttp://dx.doi.org/10.1128/AEM.66.10.4361-4365.2000Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 66, n. 10, p. 4361-4365, 2000.0099-2240http://hdl.handle.net/11449/395710.1128/AEM.66.10.4361-4365.2000WOS:000089649700028WOS000089649700028.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied and Environmental Microbiology3.6331,684info:eu-repo/semantics/openAccess2024-06-07T15:32:11Zoai:repositorio.unesp.br:11449/3957Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:48:53.541988Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial functional redundancy along a soil reclamation gradient |
title |
Bacterial functional redundancy along a soil reclamation gradient |
spellingShingle |
Bacterial functional redundancy along a soil reclamation gradient Bacterial functional redundancy along a soil reclamation gradient Yin, B. Yin, B. |
title_short |
Bacterial functional redundancy along a soil reclamation gradient |
title_full |
Bacterial functional redundancy along a soil reclamation gradient |
title_fullStr |
Bacterial functional redundancy along a soil reclamation gradient Bacterial functional redundancy along a soil reclamation gradient |
title_full_unstemmed |
Bacterial functional redundancy along a soil reclamation gradient Bacterial functional redundancy along a soil reclamation gradient |
title_sort |
Bacterial functional redundancy along a soil reclamation gradient |
author |
Yin, B. |
author_facet |
Yin, B. Yin, B. Crowley, D. Sparovek, G. De Melo, W. J. Borneman, J. Crowley, D. Sparovek, G. De Melo, W. J. Borneman, J. |
author_role |
author |
author2 |
Crowley, D. Sparovek, G. De Melo, W. J. Borneman, J. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
University of California, Riverside (UCR) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Yin, B. Crowley, D. Sparovek, G. De Melo, W. J. Borneman, J. |
description |
A strategy to measure bacterial functional redundancy was developed and tested with soils collected along a soil reclamation gradient by determining the richness and diversity of bacterial groups capable of in situ growth on selected carbon substrates. Soil cores were collected from four sites along a transect from the Jamari tin mine site in the Jamari National Forest, Rondonia, RO, Brazil: denuded mine spoil, soil from below the canopy of invading pioneer trees, revegetated soil under new growth on the forest edge, and the forest floor of an adjacent preserved forest. Bacterial population responses were analyzed by amending these soil samples with individual carbon substrates in the presence of bromodeoxyuridine (BrdU), BrdU-labeled DNA was then subjected to a 16S-23S rRNA intergenic analysis to depict the actively growing bacteria from each site, the number and diversity of bacterial groups responding to four carbon substrates (L-serine, L-threonine, sodium citrate, and or-lactose hydrate) increased along the reclamation-vegetation gradient such that the preserved forest soil samples contained the highest functional redundancy for each substrate. These data suggest that bacterial functional redundancy increases in relation to the regrowth of plant communities and may therefore represent an important aspect of the restoration of soil biological functionality to reclaimed mine spoils. They also suggest that bacterial functional redundancy may be a useful indicator of soil quality and ecosystem functioning. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-10-01 2014-05-20T13:17:31Z 2014-05-20T13:17:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/AEM.66.10.4361-4365.2000 Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 66, n. 10, p. 4361-4365, 2000. 0099-2240 http://hdl.handle.net/11449/3957 10.1128/AEM.66.10.4361-4365.2000 WOS:000089649700028 WOS000089649700028.pdf |
url |
http://dx.doi.org/10.1128/AEM.66.10.4361-4365.2000 http://hdl.handle.net/11449/3957 |
identifier_str_mv |
Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 66, n. 10, p. 4361-4365, 2000. 0099-2240 10.1128/AEM.66.10.4361-4365.2000 WOS:000089649700028 WOS000089649700028.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied and Environmental Microbiology 3.633 1,684 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4361-4365 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1822182302550065152 |
dc.identifier.doi.none.fl_str_mv |
10.1128/AEM.66.10.4361-4365.2000 |