Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)

Detalhes bibliográficos
Autor(a) principal: Pinto, A. C. R. [UNESP]
Data de Publicação: 2012
Outros Autores: Dematte, M. E. S. P. [UNESP], Creste, S., Barbosa, J. C. [UNESP], Geelen, D.
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/194762
Resumo: Tillandsia gardneri is a bromeliad with ornamental value and a wide geographical distribution over Brazil. However, due to habitat loss and illegal over-collection in the wild it is included as a vulnerable species in the official list of endangered plants of the State of Rio Grande do Sul, Brazil. The development of a protocol for T. gardneri seed propagation in vitro may be useful for reintroducing plants in their natural habitats, and for germplasm conservation. A difficult problem encountered during the establishment of an in vitro culture is explants disinfection, especially when working with endangered species, from which explant availability is restricted. Thus, the establishment of a sterilization protocol is crucial for the initiation and success of a micropropagation system for T. gardneri. The objective of this study was to evaluate the effect of sodium hypochlorite concentration and exposure time in seed and seedling surface disinfection, tissue sensitivity and development. Sodium hypochlorite solutions (10 or 20%/5, 10 or 15 min; 25%/5 or 10 min; and 50%/5 min) were effective in eliminating seed superficial contaminants. There was no significant difference among the effective sterilization treatments in relation to seed germination (%), and seedling length and number of leaves, after 120 days in vitro. Also, no damage to seed and seedling tissues were observed. Surface sterilization of seedlings, for initiation of an in vitro culture, required higher concentrations of sodium hypochlorite (25%/15 min; 20 or 50%/5, 10 or 15 min; and 40%/5 and 10 min) for controlling fungal and yeast contamination, compared to seed sterilization. No significant differences among these treatments were found in relation to seedling length and number of leaves, after 60 days in vitro.
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spelling Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)micropropagationconservationsodium hypochloriteNaOCldisinfectionTillandsia gardneri is a bromeliad with ornamental value and a wide geographical distribution over Brazil. However, due to habitat loss and illegal over-collection in the wild it is included as a vulnerable species in the official list of endangered plants of the State of Rio Grande do Sul, Brazil. The development of a protocol for T. gardneri seed propagation in vitro may be useful for reintroducing plants in their natural habitats, and for germplasm conservation. A difficult problem encountered during the establishment of an in vitro culture is explants disinfection, especially when working with endangered species, from which explant availability is restricted. Thus, the establishment of a sterilization protocol is crucial for the initiation and success of a micropropagation system for T. gardneri. The objective of this study was to evaluate the effect of sodium hypochlorite concentration and exposure time in seed and seedling surface disinfection, tissue sensitivity and development. Sodium hypochlorite solutions (10 or 20%/5, 10 or 15 min; 25%/5 or 10 min; and 50%/5 min) were effective in eliminating seed superficial contaminants. There was no significant difference among the effective sterilization treatments in relation to seed germination (%), and seedling length and number of leaves, after 120 days in vitro. Also, no damage to seed and seedling tissues were observed. Surface sterilization of seedlings, for initiation of an in vitro culture, required higher concentrations of sodium hypochlorite (25%/15 min; 20 or 50%/5, 10 or 15 min; and 40%/5 and 10 min) for controlling fungal and yeast contamination, compared to seed sterilization. No significant differences among these treatments were found in relation to seedling length and number of leaves, after 60 days in vitro.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, BrazilIAC APTA, Ctr Cana de Acucar, BR-14001970 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 08/58720-0Int Soc Horticultural ScienceUniversidade Estadual Paulista (Unesp)IAC APTAPinto, A. C. R. [UNESP]Dematte, M. E. S. P. [UNESP]Creste, S.Barbosa, J. C. [UNESP]Geelen, D.2020-12-10T16:36:48Z2020-12-10T16:36:48Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject383-389Vii International Symposium On In Vitro Culture And Horticultural Breeding. Leuven 1: Int Soc Horticultural Science, v. 961, p. 383-389, 2012.0567-7572http://hdl.handle.net/11449/194762WOS:000323607700050Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVii International Symposium On In Vitro Culture And Horticultural Breedinginfo:eu-repo/semantics/openAccess2021-10-22T20:36:18Zoai:repositorio.unesp.br:11449/194762Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:03:15.670441Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
title Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
spellingShingle Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
Pinto, A. C. R. [UNESP]
micropropagation
conservation
sodium hypochlorite
NaOCl
disinfection
title_short Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
title_full Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
title_fullStr Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
title_full_unstemmed Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
title_sort Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
author Pinto, A. C. R. [UNESP]
author_facet Pinto, A. C. R. [UNESP]
Dematte, M. E. S. P. [UNESP]
Creste, S.
Barbosa, J. C. [UNESP]
Geelen, D.
author_role author
author2 Dematte, M. E. S. P. [UNESP]
Creste, S.
Barbosa, J. C. [UNESP]
Geelen, D.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
IAC APTA
dc.contributor.author.fl_str_mv Pinto, A. C. R. [UNESP]
Dematte, M. E. S. P. [UNESP]
Creste, S.
Barbosa, J. C. [UNESP]
Geelen, D.
dc.subject.por.fl_str_mv micropropagation
conservation
sodium hypochlorite
NaOCl
disinfection
topic micropropagation
conservation
sodium hypochlorite
NaOCl
disinfection
description Tillandsia gardneri is a bromeliad with ornamental value and a wide geographical distribution over Brazil. However, due to habitat loss and illegal over-collection in the wild it is included as a vulnerable species in the official list of endangered plants of the State of Rio Grande do Sul, Brazil. The development of a protocol for T. gardneri seed propagation in vitro may be useful for reintroducing plants in their natural habitats, and for germplasm conservation. A difficult problem encountered during the establishment of an in vitro culture is explants disinfection, especially when working with endangered species, from which explant availability is restricted. Thus, the establishment of a sterilization protocol is crucial for the initiation and success of a micropropagation system for T. gardneri. The objective of this study was to evaluate the effect of sodium hypochlorite concentration and exposure time in seed and seedling surface disinfection, tissue sensitivity and development. Sodium hypochlorite solutions (10 or 20%/5, 10 or 15 min; 25%/5 or 10 min; and 50%/5 min) were effective in eliminating seed superficial contaminants. There was no significant difference among the effective sterilization treatments in relation to seed germination (%), and seedling length and number of leaves, after 120 days in vitro. Also, no damage to seed and seedling tissues were observed. Surface sterilization of seedlings, for initiation of an in vitro culture, required higher concentrations of sodium hypochlorite (25%/15 min; 20 or 50%/5, 10 or 15 min; and 40%/5 and 10 min) for controlling fungal and yeast contamination, compared to seed sterilization. No significant differences among these treatments were found in relation to seedling length and number of leaves, after 60 days in vitro.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2020-12-10T16:36:48Z
2020-12-10T16:36:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv Vii International Symposium On In Vitro Culture And Horticultural Breeding. Leuven 1: Int Soc Horticultural Science, v. 961, p. 383-389, 2012.
0567-7572
http://hdl.handle.net/11449/194762
WOS:000323607700050
identifier_str_mv Vii International Symposium On In Vitro Culture And Horticultural Breeding. Leuven 1: Int Soc Horticultural Science, v. 961, p. 383-389, 2012.
0567-7572
WOS:000323607700050
url http://hdl.handle.net/11449/194762
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vii International Symposium On In Vitro Culture And Horticultural Breeding
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 383-389
dc.publisher.none.fl_str_mv Int Soc Horticultural Science
publisher.none.fl_str_mv Int Soc Horticultural Science
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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