Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/194762 |
Resumo: | Tillandsia gardneri is a bromeliad with ornamental value and a wide geographical distribution over Brazil. However, due to habitat loss and illegal over-collection in the wild it is included as a vulnerable species in the official list of endangered plants of the State of Rio Grande do Sul, Brazil. The development of a protocol for T. gardneri seed propagation in vitro may be useful for reintroducing plants in their natural habitats, and for germplasm conservation. A difficult problem encountered during the establishment of an in vitro culture is explants disinfection, especially when working with endangered species, from which explant availability is restricted. Thus, the establishment of a sterilization protocol is crucial for the initiation and success of a micropropagation system for T. gardneri. The objective of this study was to evaluate the effect of sodium hypochlorite concentration and exposure time in seed and seedling surface disinfection, tissue sensitivity and development. Sodium hypochlorite solutions (10 or 20%/5, 10 or 15 min; 25%/5 or 10 min; and 50%/5 min) were effective in eliminating seed superficial contaminants. There was no significant difference among the effective sterilization treatments in relation to seed germination (%), and seedling length and number of leaves, after 120 days in vitro. Also, no damage to seed and seedling tissues were observed. Surface sterilization of seedlings, for initiation of an in vitro culture, required higher concentrations of sodium hypochlorite (25%/15 min; 20 or 50%/5, 10 or 15 min; and 40%/5 and 10 min) for controlling fungal and yeast contamination, compared to seed sterilization. No significant differences among these treatments were found in relation to seedling length and number of leaves, after 60 days in vitro. |
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Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae)micropropagationconservationsodium hypochloriteNaOCldisinfectionTillandsia gardneri is a bromeliad with ornamental value and a wide geographical distribution over Brazil. However, due to habitat loss and illegal over-collection in the wild it is included as a vulnerable species in the official list of endangered plants of the State of Rio Grande do Sul, Brazil. The development of a protocol for T. gardneri seed propagation in vitro may be useful for reintroducing plants in their natural habitats, and for germplasm conservation. A difficult problem encountered during the establishment of an in vitro culture is explants disinfection, especially when working with endangered species, from which explant availability is restricted. Thus, the establishment of a sterilization protocol is crucial for the initiation and success of a micropropagation system for T. gardneri. The objective of this study was to evaluate the effect of sodium hypochlorite concentration and exposure time in seed and seedling surface disinfection, tissue sensitivity and development. Sodium hypochlorite solutions (10 or 20%/5, 10 or 15 min; 25%/5 or 10 min; and 50%/5 min) were effective in eliminating seed superficial contaminants. There was no significant difference among the effective sterilization treatments in relation to seed germination (%), and seedling length and number of leaves, after 120 days in vitro. Also, no damage to seed and seedling tissues were observed. Surface sterilization of seedlings, for initiation of an in vitro culture, required higher concentrations of sodium hypochlorite (25%/15 min; 20 or 50%/5, 10 or 15 min; and 40%/5 and 10 min) for controlling fungal and yeast contamination, compared to seed sterilization. No significant differences among these treatments were found in relation to seedling length and number of leaves, after 60 days in vitro.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, BrazilIAC APTA, Ctr Cana de Acucar, BR-14001970 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 08/58720-0Int Soc Horticultural ScienceUniversidade Estadual Paulista (Unesp)IAC APTAPinto, A. C. R. [UNESP]Dematte, M. E. S. P. [UNESP]Creste, S.Barbosa, J. C. [UNESP]Geelen, D.2020-12-10T16:36:48Z2020-12-10T16:36:48Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject383-389Vii International Symposium On In Vitro Culture And Horticultural Breeding. Leuven 1: Int Soc Horticultural Science, v. 961, p. 383-389, 2012.0567-7572http://hdl.handle.net/11449/194762WOS:000323607700050Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVii International Symposium On In Vitro Culture And Horticultural Breedinginfo:eu-repo/semantics/openAccess2021-10-22T20:36:18Zoai:repositorio.unesp.br:11449/194762Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:03:15.670441Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae) |
title |
Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae) |
spellingShingle |
Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae) Pinto, A. C. R. [UNESP] micropropagation conservation sodium hypochlorite NaOCl disinfection |
title_short |
Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae) |
title_full |
Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae) |
title_fullStr |
Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae) |
title_full_unstemmed |
Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae) |
title_sort |
Seed and Seedling Surface-Sterilization for In Vitro Culture of Tillandsia gardneri (Bromeliaceae) |
author |
Pinto, A. C. R. [UNESP] |
author_facet |
Pinto, A. C. R. [UNESP] Dematte, M. E. S. P. [UNESP] Creste, S. Barbosa, J. C. [UNESP] Geelen, D. |
author_role |
author |
author2 |
Dematte, M. E. S. P. [UNESP] Creste, S. Barbosa, J. C. [UNESP] Geelen, D. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) IAC APTA |
dc.contributor.author.fl_str_mv |
Pinto, A. C. R. [UNESP] Dematte, M. E. S. P. [UNESP] Creste, S. Barbosa, J. C. [UNESP] Geelen, D. |
dc.subject.por.fl_str_mv |
micropropagation conservation sodium hypochlorite NaOCl disinfection |
topic |
micropropagation conservation sodium hypochlorite NaOCl disinfection |
description |
Tillandsia gardneri is a bromeliad with ornamental value and a wide geographical distribution over Brazil. However, due to habitat loss and illegal over-collection in the wild it is included as a vulnerable species in the official list of endangered plants of the State of Rio Grande do Sul, Brazil. The development of a protocol for T. gardneri seed propagation in vitro may be useful for reintroducing plants in their natural habitats, and for germplasm conservation. A difficult problem encountered during the establishment of an in vitro culture is explants disinfection, especially when working with endangered species, from which explant availability is restricted. Thus, the establishment of a sterilization protocol is crucial for the initiation and success of a micropropagation system for T. gardneri. The objective of this study was to evaluate the effect of sodium hypochlorite concentration and exposure time in seed and seedling surface disinfection, tissue sensitivity and development. Sodium hypochlorite solutions (10 or 20%/5, 10 or 15 min; 25%/5 or 10 min; and 50%/5 min) were effective in eliminating seed superficial contaminants. There was no significant difference among the effective sterilization treatments in relation to seed germination (%), and seedling length and number of leaves, after 120 days in vitro. Also, no damage to seed and seedling tissues were observed. Surface sterilization of seedlings, for initiation of an in vitro culture, required higher concentrations of sodium hypochlorite (25%/15 min; 20 or 50%/5, 10 or 15 min; and 40%/5 and 10 min) for controlling fungal and yeast contamination, compared to seed sterilization. No significant differences among these treatments were found in relation to seedling length and number of leaves, after 60 days in vitro. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2020-12-10T16:36:48Z 2020-12-10T16:36:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Vii International Symposium On In Vitro Culture And Horticultural Breeding. Leuven 1: Int Soc Horticultural Science, v. 961, p. 383-389, 2012. 0567-7572 http://hdl.handle.net/11449/194762 WOS:000323607700050 |
identifier_str_mv |
Vii International Symposium On In Vitro Culture And Horticultural Breeding. Leuven 1: Int Soc Horticultural Science, v. 961, p. 383-389, 2012. 0567-7572 WOS:000323607700050 |
url |
http://hdl.handle.net/11449/194762 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vii International Symposium On In Vitro Culture And Horticultural Breeding |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
383-389 |
dc.publisher.none.fl_str_mv |
Int Soc Horticultural Science |
publisher.none.fl_str_mv |
Int Soc Horticultural Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129387834376192 |