Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato

Detalhes bibliográficos
Autor(a) principal: Silva, Gisela Bevilacqua Rolfsen Ferreira da [UNESP]
Data de Publicação: 2013
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/108421
Resumo: The jasmonates are a new family of anti-cancer agents, natural and semi-synthetic, with proven efficacy against several tumor growth. These compounds exhibit a selective cytotoxic for tumor cells. Colloidal nanocarriers as micro (ME) and nanoemulsions (NE), provide drug release at the desired site of action while minimizing the side effects that often follow the use of conventional drugs. The methyl dihydro jasmonate (MJ) was added in order to obtain nanostructured systems with antineoplastic activity. Three phase diagrams were developed, one of them without drug, the second with drug and the third with MJ replacing the oil phase. Based on the phase diagrams 32 formulations were selected by varying the percentage of the oily phase (soya oil), of the surfactants (fatty acids salts, soya phosphatidylcholine and glycerol) and of the MJ. The formulations were analyzed by their physico-chemical characterization. With the technique of light scattering it was observed that the diameter of the droplets increased with the incorporation of the drug and with the increase of the MJ percentage. By increasing the surfactants percentage, the droplet diameter decreased in formulations without MJ and increased in formulations with the drug. With the oil phase increase the droplet diameter was reduced in formulations with and without MJ. The rheology evaluation syudied revealed that the flow behavior of the formulations ranged as newtonian, pseudoplastic, thixotropic, anti-thixotropic and reopetic. Based on the polarized light microscopy it was observed that the formulations, with and without MJ showed dark field with rare presence of Maltese cross. In the formulations where MJ was used as the oil phase, the presence of microscopic oil droplets was observed. The majority of the formulations exhibit characteristics of crystalline structures by X-ray diffraction. The SAXS curves show that, in the formulations without MJ, structural organization occurred when the oil phase was increased, the percentage of surfactants and the percentage of drug increased. A disruption has occurred when MJ was incorporated in the formulations. The zeta potential decreased in module when MJ was incorporated in the formulations and when the percentage of the drug increased. However, the zeta potential increased in module, in the formulations having MJ as the oil phase, as the percentage of oil phase and surfactant increased. In the in vitro release assay the ME and NE systems behaved as reservoirs delaying the release of MJ. Through the analysis of antitumor activity in vivo it can be observed that the percentage inhibition of tumor volume of MJ micellar solution exceeded the positive control, doxorubicin. As the concentration of MJ-ME increasing the percentage of tumor inhibition remained almost constant. The angiogenesis degree was lower for doxorubicin and micellar solution compared to the saline and the formulation without MJ. However, the groups treated with MJ-ME revealed a high degree of angiogenesis. The average weight of the tumors was higher in the groups treated with saline and with the formulation without MJ and lower for those treated with doxorubicin and MJ micellar solution. As for the groups treated with MJME there was reduction of the weight tumors as the MJ concentration increased on the ME. It was possible to obtain sustained release systems, micro and nanoemulsions, able to carry and direct the MJ in order to reach tumor cells allowing intravenous administration.
id UNSP_7f7658536b4dfdb4ae13d49764d3e6f5
oai_identifier_str oai:repositorio.unesp.br:11449/108421
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonatoAgentes antineoplasicosFármacosReologiaMicroscopia de polarizaçãoCancerRheologyThe jasmonates are a new family of anti-cancer agents, natural and semi-synthetic, with proven efficacy against several tumor growth. These compounds exhibit a selective cytotoxic for tumor cells. Colloidal nanocarriers as micro (ME) and nanoemulsions (NE), provide drug release at the desired site of action while minimizing the side effects that often follow the use of conventional drugs. The methyl dihydro jasmonate (MJ) was added in order to obtain nanostructured systems with antineoplastic activity. Three phase diagrams were developed, one of them without drug, the second with drug and the third with MJ replacing the oil phase. Based on the phase diagrams 32 formulations were selected by varying the percentage of the oily phase (soya oil), of the surfactants (fatty acids salts, soya phosphatidylcholine and glycerol) and of the MJ. The formulations were analyzed by their physico-chemical characterization. With the technique of light scattering it was observed that the diameter of the droplets increased with the incorporation of the drug and with the increase of the MJ percentage. By increasing the surfactants percentage, the droplet diameter decreased in formulations without MJ and increased in formulations with the drug. With the oil phase increase the droplet diameter was reduced in formulations with and without MJ. The rheology evaluation syudied revealed that the flow behavior of the formulations ranged as newtonian, pseudoplastic, thixotropic, anti-thixotropic and reopetic. Based on the polarized light microscopy it was observed that the formulations, with and without MJ showed dark field with rare presence of Maltese cross. In the formulations where MJ was used as the oil phase, the presence of microscopic oil droplets was observed. The majority of the formulations exhibit characteristics of crystalline structures by X-ray diffraction. The SAXS curves show that, in the formulations without MJ, structural organization occurred when the oil phase was increased, the percentage of surfactants and the percentage of drug increased. A disruption has occurred when MJ was incorporated in the formulations. The zeta potential decreased in module when MJ was incorporated in the formulations and when the percentage of the drug increased. However, the zeta potential increased in module, in the formulations having MJ as the oil phase, as the percentage of oil phase and surfactant increased. In the in vitro release assay the ME and NE systems behaved as reservoirs delaying the release of MJ. Through the analysis of antitumor activity in vivo it can be observed that the percentage inhibition of tumor volume of MJ micellar solution exceeded the positive control, doxorubicin. As the concentration of MJ-ME increasing the percentage of tumor inhibition remained almost constant. The angiogenesis degree was lower for doxorubicin and micellar solution compared to the saline and the formulation without MJ. However, the groups treated with MJ-ME revealed a high degree of angiogenesis. The average weight of the tumors was higher in the groups treated with saline and with the formulation without MJ and lower for those treated with doxorubicin and MJ micellar solution. As for the groups treated with MJME there was reduction of the weight tumors as the MJ concentration increased on the ME. It was possible to obtain sustained release systems, micro and nanoemulsions, able to carry and direct the MJ in order to reach tumor cells allowing intravenous administration.Os jasmonatos compõem uma nova família de agentes anti-câncer, naturais ou semisintéticos, com comprovada eficácia contra diferentes tipos de tumores em crescimento. Esses compostos exibem seletividade citotóxica em células transformadas. Nanocarreadores coloidais como micro e nanoemulsões, podem proporcionar a liberação do fármaco no sítio de ação desejado minimizando os efeitos colaterais que normalmente acompanham os medicamentos convencionais. Neste trabalho foram desenvolvidas formulações de micro (ME) e nanoemulsões (NE) contendo o fármaco metildiidrojasmonato (MJ) com o objetivo de obter sistemas nanoestruturados com atividade antineoplásica. Foram desenvolvidos três diagramas de fases, o primeiro sem MJ, o segundo contendo MJ e o terceiro com MJ como fase oleosa. Com base nos diagramas 32 formulações foram selecionadas, variando-se a proporção de fase oleosa (óleo de soja), de tensoativo (sais de ácidos graxos, fosfatidilcolina de soja e glicerol) e de MJ. As formulações foram caracterizadas físico-quimicamente e com a técnica de light scattering foi observado que o diâmetro das gotículas aumentou com a incorporação do fármaco e com o aumento da proporção do MJ. Com o aumento da proporção de tensoativo o diâmetro diminuiu nas formulações na ausência do fármaco e aumentou nas formulações com o fármaco incorporado. Com o aumento de fase oleosa o diâmetro foi reduzindo nas formulações na ausência e presença de fármaco. Com as análises de reologia foi possível observar que o comportamento de fluxo destas formulações variou entre newtoniano, pseudoplástico, tixotrópico, anti-tixotrópico e reopético. Com base na microscopia de luz polarizada foi possível observar que as formulações na ausência e presença do fármaco e aquelas com variação de MJ apresentaram campo escuro com rara presença de cruz de malta. Já as formulações em que MJ foi utilizado como fase oleosa ocorreu presença de gotas de óleo na microscopia de luz polarizada. A maioria das formulações apresentou características de estruturas cristalinas através da difração de raios-X. As curvas de SAXS demonstram que nas formulações na ausência de MJ ocorreu uma organização estrutural à medida que se aumentou a fase oleosa, proporção de tensoativo e proporção de fármaco. A incorporação de MJ promoveu uma desestruturação organizacional. O potencial zeta sofreu uma queda em módulo quando MJ foi incorporado nas formulações e quando a proporção deste fármaco foi aumentada. Mas o potencial zeta sofreu aumento em módulo nas formulações tendo MJ como fase oleosa, à medida que se aumentou proporção de fase oleosa e tensoativo. No ensaio de liberação in vitro as ME e NE comportaram-se como sistemas reservatórios retardando a liberação do MJ. Com a análise da atividade antitumoral in vivo foi observado que a porcentagem de inibição do volume tumoral da solução micelar de MJ (SM-MJ) superou a do controle positivo, com doxorrubicina. À medida que a concentração de MJ incorporado a microemulsão (MJ-ME) foi aumentando a porcentagem de inibição tumoral manteve-se praticamente constante. O grau de angiogênese foi mais baixo para a doxorrubicina e solução micelar de MJ quando comparado com salina e ME vazia. Já para os grupos tratados com MJ-ME os valores do grau de angiogênese mantiveram-se altos. A média da massa dos tumores foi maior para os grupos tratados com salina e ME vazia e menor para os tratados com doxorrubicina e solução micelar de MJ. Já os grupos tratados com MJ-ME obtiveram redução na massa à medida que a concentração de MJ-ME aumentou. Foi possível obter sistemas de liberação prolongada, micro e nanoemulsões, capazes de transportar e direcionar o MJ no organismo de modo a atingir as células tumorais permitindo administração intravenosa.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Oliveira, Anselmo Gomes de [UNESP]Universidade Estadual Paulista (Unesp)Silva, Gisela Bevilacqua Rolfsen Ferreira da [UNESP]2014-08-13T14:50:35Z2014-08-13T14:50:35Z2013-10-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis165 f. : grafs.application/pdfSILVA, Gisela Bevilacqua Rolfsen Ferreira da. Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato. 2013. 165 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2013.http://hdl.handle.net/11449/108421000736216000736216.pdf52001016048P09114495952533044Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-06-24T19:19:12Zoai:repositorio.unesp.br:11449/108421Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:51:43.193131Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato
title Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato
spellingShingle Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato
Silva, Gisela Bevilacqua Rolfsen Ferreira da [UNESP]
Agentes antineoplasicos
Fármacos
Reologia
Microscopia de polarização
Cancer
Rheology
title_short Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato
title_full Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato
title_fullStr Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato
title_full_unstemmed Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato
title_sort Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato
author Silva, Gisela Bevilacqua Rolfsen Ferreira da [UNESP]
author_facet Silva, Gisela Bevilacqua Rolfsen Ferreira da [UNESP]
author_role author
dc.contributor.none.fl_str_mv Oliveira, Anselmo Gomes de [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Gisela Bevilacqua Rolfsen Ferreira da [UNESP]
dc.subject.por.fl_str_mv Agentes antineoplasicos
Fármacos
Reologia
Microscopia de polarização
Cancer
Rheology
topic Agentes antineoplasicos
Fármacos
Reologia
Microscopia de polarização
Cancer
Rheology
description The jasmonates are a new family of anti-cancer agents, natural and semi-synthetic, with proven efficacy against several tumor growth. These compounds exhibit a selective cytotoxic for tumor cells. Colloidal nanocarriers as micro (ME) and nanoemulsions (NE), provide drug release at the desired site of action while minimizing the side effects that often follow the use of conventional drugs. The methyl dihydro jasmonate (MJ) was added in order to obtain nanostructured systems with antineoplastic activity. Three phase diagrams were developed, one of them without drug, the second with drug and the third with MJ replacing the oil phase. Based on the phase diagrams 32 formulations were selected by varying the percentage of the oily phase (soya oil), of the surfactants (fatty acids salts, soya phosphatidylcholine and glycerol) and of the MJ. The formulations were analyzed by their physico-chemical characterization. With the technique of light scattering it was observed that the diameter of the droplets increased with the incorporation of the drug and with the increase of the MJ percentage. By increasing the surfactants percentage, the droplet diameter decreased in formulations without MJ and increased in formulations with the drug. With the oil phase increase the droplet diameter was reduced in formulations with and without MJ. The rheology evaluation syudied revealed that the flow behavior of the formulations ranged as newtonian, pseudoplastic, thixotropic, anti-thixotropic and reopetic. Based on the polarized light microscopy it was observed that the formulations, with and without MJ showed dark field with rare presence of Maltese cross. In the formulations where MJ was used as the oil phase, the presence of microscopic oil droplets was observed. The majority of the formulations exhibit characteristics of crystalline structures by X-ray diffraction. The SAXS curves show that, in the formulations without MJ, structural organization occurred when the oil phase was increased, the percentage of surfactants and the percentage of drug increased. A disruption has occurred when MJ was incorporated in the formulations. The zeta potential decreased in module when MJ was incorporated in the formulations and when the percentage of the drug increased. However, the zeta potential increased in module, in the formulations having MJ as the oil phase, as the percentage of oil phase and surfactant increased. In the in vitro release assay the ME and NE systems behaved as reservoirs delaying the release of MJ. Through the analysis of antitumor activity in vivo it can be observed that the percentage inhibition of tumor volume of MJ micellar solution exceeded the positive control, doxorubicin. As the concentration of MJ-ME increasing the percentage of tumor inhibition remained almost constant. The angiogenesis degree was lower for doxorubicin and micellar solution compared to the saline and the formulation without MJ. However, the groups treated with MJ-ME revealed a high degree of angiogenesis. The average weight of the tumors was higher in the groups treated with saline and with the formulation without MJ and lower for those treated with doxorubicin and MJ micellar solution. As for the groups treated with MJME there was reduction of the weight tumors as the MJ concentration increased on the ME. It was possible to obtain sustained release systems, micro and nanoemulsions, able to carry and direct the MJ in order to reach tumor cells allowing intravenous administration.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-10
2014-08-13T14:50:35Z
2014-08-13T14:50:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Gisela Bevilacqua Rolfsen Ferreira da. Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato. 2013. 165 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2013.
http://hdl.handle.net/11449/108421
000736216
000736216.pdf
52001016048P0
9114495952533044
identifier_str_mv SILVA, Gisela Bevilacqua Rolfsen Ferreira da. Desenvolvimento e caracterização físico-química e biofarmacêutica de nano e microemulsões lipídicas de uso intravenoso contendo metildiidrojasmonato. 2013. 165 f. Tese (doutorado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2013.
000736216
000736216.pdf
52001016048P0
9114495952533044
url http://hdl.handle.net/11449/108421
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 165 f. : grafs.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128711188283392