In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12155-023-10594-7 http://hdl.handle.net/11449/247118 |
Resumo: | White-rot fungi (WRF) synthesize ligninolytic extracellular oxidative enzymes such as laccase (Lcc), which has been described as one of the most interesting types of redox enzymes that can improve microbial fuel cell (MFC) performance. Therefore, and in order to test that performance, WRF Trametes versicolor MUM 04.100 was immobilized in nylon sponge and fixed in the MFC cathode chamber, while Lcc activity, bioelectricity production, and organic matter removal were monitored. It was found that current density measured in the MFC supplemented with fungi was 2.1 times higher (42.81 ± 4.91 mA/m2) than current density obtained in the control MFC (absence of fungus in the cathode chamber, 20.31 ± 4.30 mA/m2). Maximum Lcc activity (23.08 U/L) and the highest value of organic matter removal (COD) (92%) from domestic wastewater was obtained on the last cycle after biofilm maturation and glycerol pulse. This work evidences that Lcc continuously synthesized by MUM 04.100 immobilized in the biocathode is a promising approach to enhance MFC power performance and wastewater treatment. |
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In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel CellsBioelectricityFungal enzyme immobilizationGlycerolMunicipal wastewaterWhite-rot fungi (WRF) synthesize ligninolytic extracellular oxidative enzymes such as laccase (Lcc), which has been described as one of the most interesting types of redox enzymes that can improve microbial fuel cell (MFC) performance. Therefore, and in order to test that performance, WRF Trametes versicolor MUM 04.100 was immobilized in nylon sponge and fixed in the MFC cathode chamber, while Lcc activity, bioelectricity production, and organic matter removal were monitored. It was found that current density measured in the MFC supplemented with fungi was 2.1 times higher (42.81 ± 4.91 mA/m2) than current density obtained in the control MFC (absence of fungus in the cathode chamber, 20.31 ± 4.30 mA/m2). Maximum Lcc activity (23.08 U/L) and the highest value of organic matter removal (COD) (92%) from domestic wastewater was obtained on the last cycle after biofilm maturation and glycerol pulse. This work evidences that Lcc continuously synthesized by MUM 04.100 immobilized in the biocathode is a promising approach to enhance MFC power performance and wastewater treatment.São Paulo State University (UNESP) Institute of BiosciencesLEAF-Linking Landscape Environment Agriculture and Food Research Center Associated Laboratory TERRA Instituto Superior de Agronomia Universidade de Lisboa Tapada da AjudaInstitute of Advanced Sea Studies (IEAMAR) São Paulo State University (UNESP), SPCentre of Biological Engineering University of Minho, Campus of GualtarIndustrial Biotechnology Laboratory Institute of Technological Research of São Paulo State, São PauloSão Paulo State University (UNESP) Institute of BiosciencesInstitute of Advanced Sea Studies (IEAMAR) São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Tapada da AjudaUniversity of MinhoInstitute of Technological Research of São Paulo StateOttoni, Cristiane [UNESP]do Valle Trotta, Caterina [UNESP]Martins, GilbertoMatos, JoanaMaiorano, Alfredo EduardoBrito, António G.Peixoto, Luciana2023-07-29T13:06:43Z2023-07-29T13:06:43Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s12155-023-10594-7Bioenergy Research.1939-12421939-1234http://hdl.handle.net/11449/24711810.1007/s12155-023-10594-72-s2.0-85151717375Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioenergy Researchinfo:eu-repo/semantics/openAccess2023-07-29T13:06:43Zoai:repositorio.unesp.br:11449/247118Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:53:53.608453Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells |
title |
In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells |
spellingShingle |
In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells Ottoni, Cristiane [UNESP] Bioelectricity Fungal enzyme immobilization Glycerol Municipal wastewater |
title_short |
In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells |
title_full |
In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells |
title_fullStr |
In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells |
title_full_unstemmed |
In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells |
title_sort |
In Situ Trametes versicolor Laccase Biocathode Performance Assessment in Dual-Chamber Microbial Fuel Cells |
author |
Ottoni, Cristiane [UNESP] |
author_facet |
Ottoni, Cristiane [UNESP] do Valle Trotta, Caterina [UNESP] Martins, Gilberto Matos, Joana Maiorano, Alfredo Eduardo Brito, António G. Peixoto, Luciana |
author_role |
author |
author2 |
do Valle Trotta, Caterina [UNESP] Martins, Gilberto Matos, Joana Maiorano, Alfredo Eduardo Brito, António G. Peixoto, Luciana |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Tapada da Ajuda University of Minho Institute of Technological Research of São Paulo State |
dc.contributor.author.fl_str_mv |
Ottoni, Cristiane [UNESP] do Valle Trotta, Caterina [UNESP] Martins, Gilberto Matos, Joana Maiorano, Alfredo Eduardo Brito, António G. Peixoto, Luciana |
dc.subject.por.fl_str_mv |
Bioelectricity Fungal enzyme immobilization Glycerol Municipal wastewater |
topic |
Bioelectricity Fungal enzyme immobilization Glycerol Municipal wastewater |
description |
White-rot fungi (WRF) synthesize ligninolytic extracellular oxidative enzymes such as laccase (Lcc), which has been described as one of the most interesting types of redox enzymes that can improve microbial fuel cell (MFC) performance. Therefore, and in order to test that performance, WRF Trametes versicolor MUM 04.100 was immobilized in nylon sponge and fixed in the MFC cathode chamber, while Lcc activity, bioelectricity production, and organic matter removal were monitored. It was found that current density measured in the MFC supplemented with fungi was 2.1 times higher (42.81 ± 4.91 mA/m2) than current density obtained in the control MFC (absence of fungus in the cathode chamber, 20.31 ± 4.30 mA/m2). Maximum Lcc activity (23.08 U/L) and the highest value of organic matter removal (COD) (92%) from domestic wastewater was obtained on the last cycle after biofilm maturation and glycerol pulse. This work evidences that Lcc continuously synthesized by MUM 04.100 immobilized in the biocathode is a promising approach to enhance MFC power performance and wastewater treatment. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:06:43Z 2023-07-29T13:06:43Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12155-023-10594-7 Bioenergy Research. 1939-1242 1939-1234 http://hdl.handle.net/11449/247118 10.1007/s12155-023-10594-7 2-s2.0-85151717375 |
url |
http://dx.doi.org/10.1007/s12155-023-10594-7 http://hdl.handle.net/11449/247118 |
identifier_str_mv |
Bioenergy Research. 1939-1242 1939-1234 10.1007/s12155-023-10594-7 2-s2.0-85151717375 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioenergy Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128580467556352 |