Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jembe.2021.151547 http://hdl.handle.net/11449/206078 |
Resumo: | In semi-terrestrial crustaceans, mothers and the carried embryos form a unit. The ideal conditions for embryonic development place a sustained demand on the mothers' energy budget. A particular challenge for this unit is the transition between terrestrial and aquatic environments. Ecophysiological information on mothers and embryos contributes to the understanding of evolutionary pathways and constraints of terrestrialization in crabs. We evaluated the effects of 6, 12, and 18 h of water deprivation on embryonic development and maternal physiology of the tree-climbing crab Aratus pisonii (H. Milne Edwards, 1837). Specifically, we determined the effect of water deprivation on egg volume, osmolality, and larval survival, as well as on maternal body mass, hemolymph osmolality, and branchial carbonic anhydrase activity (CAA). We hypothesized that longer periods of water deprivation would negatively affect larvae viability, as well as disrupt the physiology of brooding females. Embryonic development, larval survival, and the mother's physiology were only altered after 18 h of water deprivation, possibly due to dehydration and/or the accumulation of metabolic wastes that could not be excreted in the absence of water. Osmolality was higher in females than in their eggs. CAA of the anterior gills was higher in ovigerous than in non-ovigerous females, possibly due to the increase in respiratory demands caused by carrying eggs. Our results confirmed that the gestation period represents a metabolically expensive period for mother crabs and that water deprivation of 18 h was critical for the embryos' survival. |
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Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisoniiCarbonic anhydraseDehydrationLarval survivalOsmolalityOsmotic stressTerrestrializationIn semi-terrestrial crustaceans, mothers and the carried embryos form a unit. The ideal conditions for embryonic development place a sustained demand on the mothers' energy budget. A particular challenge for this unit is the transition between terrestrial and aquatic environments. Ecophysiological information on mothers and embryos contributes to the understanding of evolutionary pathways and constraints of terrestrialization in crabs. We evaluated the effects of 6, 12, and 18 h of water deprivation on embryonic development and maternal physiology of the tree-climbing crab Aratus pisonii (H. Milne Edwards, 1837). Specifically, we determined the effect of water deprivation on egg volume, osmolality, and larval survival, as well as on maternal body mass, hemolymph osmolality, and branchial carbonic anhydrase activity (CAA). We hypothesized that longer periods of water deprivation would negatively affect larvae viability, as well as disrupt the physiology of brooding females. Embryonic development, larval survival, and the mother's physiology were only altered after 18 h of water deprivation, possibly due to dehydration and/or the accumulation of metabolic wastes that could not be excreted in the absence of water. Osmolality was higher in females than in their eggs. CAA of the anterior gills was higher in ovigerous than in non-ovigerous females, possibly due to the increase in respiratory demands caused by carrying eggs. Our results confirmed that the gestation period represents a metabolically expensive period for mother crabs and that water deprivation of 18 h was critical for the embryos' survival.Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais RenováveisSão Paulo State University UNESP Biosciences Institute, Coastal CampusDepartment of Physiology Federal University of ParanáDepartment of Zoology Federal University of ParanáSão Paulo State University UNESP Biosciences Institute, Coastal CampusInstituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis: # 34355-1Universidade Estadual Paulista (Unesp)Federal University of ParanáMarochi, Murilo Zanetti [UNESP]Castellano, Giovanna CarstensFreire, Carolina ArrudaMasunari, Setuko2021-06-25T10:26:14Z2021-06-25T10:26:14Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jembe.2021.151547Journal of Experimental Marine Biology and Ecology, v. 540.0022-0981http://hdl.handle.net/11449/20607810.1016/j.jembe.2021.1515472-s2.0-85102853086Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Experimental Marine Biology and Ecologyinfo:eu-repo/semantics/openAccess2021-10-22T20:49:13Zoai:repositorio.unesp.br:11449/206078Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:49:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii |
title |
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii |
spellingShingle |
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii Marochi, Murilo Zanetti [UNESP] Carbonic anhydrase Dehydration Larval survival Osmolality Osmotic stress Terrestrialization |
title_short |
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii |
title_full |
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii |
title_fullStr |
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii |
title_full_unstemmed |
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii |
title_sort |
Carrying eggs in a semi-terrestrial environment: Physiological responses to water deprivation of mothers and embryos of the tree-climbing crab Aratus pisonii |
author |
Marochi, Murilo Zanetti [UNESP] |
author_facet |
Marochi, Murilo Zanetti [UNESP] Castellano, Giovanna Carstens Freire, Carolina Arruda Masunari, Setuko |
author_role |
author |
author2 |
Castellano, Giovanna Carstens Freire, Carolina Arruda Masunari, Setuko |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Paraná |
dc.contributor.author.fl_str_mv |
Marochi, Murilo Zanetti [UNESP] Castellano, Giovanna Carstens Freire, Carolina Arruda Masunari, Setuko |
dc.subject.por.fl_str_mv |
Carbonic anhydrase Dehydration Larval survival Osmolality Osmotic stress Terrestrialization |
topic |
Carbonic anhydrase Dehydration Larval survival Osmolality Osmotic stress Terrestrialization |
description |
In semi-terrestrial crustaceans, mothers and the carried embryos form a unit. The ideal conditions for embryonic development place a sustained demand on the mothers' energy budget. A particular challenge for this unit is the transition between terrestrial and aquatic environments. Ecophysiological information on mothers and embryos contributes to the understanding of evolutionary pathways and constraints of terrestrialization in crabs. We evaluated the effects of 6, 12, and 18 h of water deprivation on embryonic development and maternal physiology of the tree-climbing crab Aratus pisonii (H. Milne Edwards, 1837). Specifically, we determined the effect of water deprivation on egg volume, osmolality, and larval survival, as well as on maternal body mass, hemolymph osmolality, and branchial carbonic anhydrase activity (CAA). We hypothesized that longer periods of water deprivation would negatively affect larvae viability, as well as disrupt the physiology of brooding females. Embryonic development, larval survival, and the mother's physiology were only altered after 18 h of water deprivation, possibly due to dehydration and/or the accumulation of metabolic wastes that could not be excreted in the absence of water. Osmolality was higher in females than in their eggs. CAA of the anterior gills was higher in ovigerous than in non-ovigerous females, possibly due to the increase in respiratory demands caused by carrying eggs. Our results confirmed that the gestation period represents a metabolically expensive period for mother crabs and that water deprivation of 18 h was critical for the embryos' survival. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:26:14Z 2021-06-25T10:26:14Z 2021-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jembe.2021.151547 Journal of Experimental Marine Biology and Ecology, v. 540. 0022-0981 http://hdl.handle.net/11449/206078 10.1016/j.jembe.2021.151547 2-s2.0-85102853086 |
url |
http://dx.doi.org/10.1016/j.jembe.2021.151547 http://hdl.handle.net/11449/206078 |
identifier_str_mv |
Journal of Experimental Marine Biology and Ecology, v. 540. 0022-0981 10.1016/j.jembe.2021.151547 2-s2.0-85102853086 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Experimental Marine Biology and Ecology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961721448529920 |