Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pntd.0006621 http://hdl.handle.net/11449/180065 |
Resumo: | Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis. |
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spelling |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira sppLeptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis.Department of Support Production and Animal Health São Paulo State University (Unesp) School of Veterinary MedicineDepartment of Clinic Surgery and Animal Reproduction São Paulo State University (Unesp) School of Veterinary MedicineDepartment of Support Production and Animal Health São Paulo State University (Unesp) School of Veterinary MedicineDepartment of Clinic Surgery and Animal Reproduction São Paulo State University (Unesp) School of Veterinary MedicineUniversidade Estadual Paulista (Unesp)Garcia, Leandro Encarnação [UNESP]de Araújo Junior, Erivelto Corrêa [UNESP]Melo, Larissa Martins [UNESP]Bragato, Jaqueline Poleto [UNESP]Peiró, Juliana Regina [UNESP]Félix de Lima, Valéria Marçal [UNESP]Marinho, Márcia [UNESP]Arnold, Daniel Robert [UNESP]Lopes, Flavia Lombardi [UNESP]2018-12-11T17:37:52Z2018-12-11T17:37:52Z2018-07-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pntd.0006621PLoS Neglected Tropical Diseases, v. 12, n. 7, 2018.1935-27351935-2727http://hdl.handle.net/11449/18006510.1371/journal.pntd.00066212-s2.0-850507254072-s2.0-85050725407.pdf5071093931017493Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS Neglected Tropical Diseases2,5892,589info:eu-repo/semantics/openAccess2024-09-04T19:15:38Zoai:repositorio.unesp.br:11449/180065Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp |
title |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp |
spellingShingle |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp Garcia, Leandro Encarnação [UNESP] |
title_short |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp |
title_full |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp |
title_fullStr |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp |
title_full_unstemmed |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp |
title_sort |
Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp |
author |
Garcia, Leandro Encarnação [UNESP] |
author_facet |
Garcia, Leandro Encarnação [UNESP] de Araújo Junior, Erivelto Corrêa [UNESP] Melo, Larissa Martins [UNESP] Bragato, Jaqueline Poleto [UNESP] Peiró, Juliana Regina [UNESP] Félix de Lima, Valéria Marçal [UNESP] Marinho, Márcia [UNESP] Arnold, Daniel Robert [UNESP] Lopes, Flavia Lombardi [UNESP] |
author_role |
author |
author2 |
de Araújo Junior, Erivelto Corrêa [UNESP] Melo, Larissa Martins [UNESP] Bragato, Jaqueline Poleto [UNESP] Peiró, Juliana Regina [UNESP] Félix de Lima, Valéria Marçal [UNESP] Marinho, Márcia [UNESP] Arnold, Daniel Robert [UNESP] Lopes, Flavia Lombardi [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Garcia, Leandro Encarnação [UNESP] de Araújo Junior, Erivelto Corrêa [UNESP] Melo, Larissa Martins [UNESP] Bragato, Jaqueline Poleto [UNESP] Peiró, Juliana Regina [UNESP] Félix de Lima, Valéria Marçal [UNESP] Marinho, Márcia [UNESP] Arnold, Daniel Robert [UNESP] Lopes, Flavia Lombardi [UNESP] |
description |
Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:37:52Z 2018-12-11T17:37:52Z 2018-07-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pntd.0006621 PLoS Neglected Tropical Diseases, v. 12, n. 7, 2018. 1935-2735 1935-2727 http://hdl.handle.net/11449/180065 10.1371/journal.pntd.0006621 2-s2.0-85050725407 2-s2.0-85050725407.pdf 5071093931017493 |
url |
http://dx.doi.org/10.1371/journal.pntd.0006621 http://hdl.handle.net/11449/180065 |
identifier_str_mv |
PLoS Neglected Tropical Diseases, v. 12, n. 7, 2018. 1935-2735 1935-2727 10.1371/journal.pntd.0006621 2-s2.0-85050725407 2-s2.0-85050725407.pdf 5071093931017493 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS Neglected Tropical Diseases 2,589 2,589 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021391795224576 |