Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp

Detalhes bibliográficos
Autor(a) principal: Garcia, Leandro Encarnação [UNESP]
Data de Publicação: 2018
Outros Autores: de Araújo Junior, Erivelto Corrêa [UNESP], Melo, Larissa Martins [UNESP], Bragato, Jaqueline Poleto [UNESP], Peiró, Juliana Regina [UNESP], Félix de Lima, Valéria Marçal [UNESP], Marinho, Márcia [UNESP], Arnold, Daniel Robert [UNESP], Lopes, Flavia Lombardi [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pntd.0006621
http://hdl.handle.net/11449/180065
Resumo: Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis.
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spelling Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira sppLeptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis.Department of Support Production and Animal Health São Paulo State University (Unesp) School of Veterinary MedicineDepartment of Clinic Surgery and Animal Reproduction São Paulo State University (Unesp) School of Veterinary MedicineDepartment of Support Production and Animal Health São Paulo State University (Unesp) School of Veterinary MedicineDepartment of Clinic Surgery and Animal Reproduction São Paulo State University (Unesp) School of Veterinary MedicineUniversidade Estadual Paulista (Unesp)Garcia, Leandro Encarnação [UNESP]de Araújo Junior, Erivelto Corrêa [UNESP]Melo, Larissa Martins [UNESP]Bragato, Jaqueline Poleto [UNESP]Peiró, Juliana Regina [UNESP]Félix de Lima, Valéria Marçal [UNESP]Marinho, Márcia [UNESP]Arnold, Daniel Robert [UNESP]Lopes, Flavia Lombardi [UNESP]2018-12-11T17:37:52Z2018-12-11T17:37:52Z2018-07-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pntd.0006621PLoS Neglected Tropical Diseases, v. 12, n. 7, 2018.1935-27351935-2727http://hdl.handle.net/11449/18006510.1371/journal.pntd.00066212-s2.0-850507254072-s2.0-85050725407.pdf5071093931017493Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS Neglected Tropical Diseases2,5892,589info:eu-repo/semantics/openAccess2024-09-04T19:15:38Zoai:repositorio.unesp.br:11449/180065Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
title Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
spellingShingle Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
Garcia, Leandro Encarnação [UNESP]
title_short Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
title_full Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
title_fullStr Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
title_full_unstemmed Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
title_sort Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp
author Garcia, Leandro Encarnação [UNESP]
author_facet Garcia, Leandro Encarnação [UNESP]
de Araújo Junior, Erivelto Corrêa [UNESP]
Melo, Larissa Martins [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Peiró, Juliana Regina [UNESP]
Félix de Lima, Valéria Marçal [UNESP]
Marinho, Márcia [UNESP]
Arnold, Daniel Robert [UNESP]
Lopes, Flavia Lombardi [UNESP]
author_role author
author2 de Araújo Junior, Erivelto Corrêa [UNESP]
Melo, Larissa Martins [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Peiró, Juliana Regina [UNESP]
Félix de Lima, Valéria Marçal [UNESP]
Marinho, Márcia [UNESP]
Arnold, Daniel Robert [UNESP]
Lopes, Flavia Lombardi [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Garcia, Leandro Encarnação [UNESP]
de Araújo Junior, Erivelto Corrêa [UNESP]
Melo, Larissa Martins [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Peiró, Juliana Regina [UNESP]
Félix de Lima, Valéria Marçal [UNESP]
Marinho, Márcia [UNESP]
Arnold, Daniel Robert [UNESP]
Lopes, Flavia Lombardi [UNESP]
description Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:37:52Z
2018-12-11T17:37:52Z
2018-07-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pntd.0006621
PLoS Neglected Tropical Diseases, v. 12, n. 7, 2018.
1935-2735
1935-2727
http://hdl.handle.net/11449/180065
10.1371/journal.pntd.0006621
2-s2.0-85050725407
2-s2.0-85050725407.pdf
5071093931017493
url http://dx.doi.org/10.1371/journal.pntd.0006621
http://hdl.handle.net/11449/180065
identifier_str_mv PLoS Neglected Tropical Diseases, v. 12, n. 7, 2018.
1935-2735
1935-2727
10.1371/journal.pntd.0006621
2-s2.0-85050725407
2-s2.0-85050725407.pdf
5071093931017493
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS Neglected Tropical Diseases
2,589
2,589
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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