Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/232166 |
Resumo: | Twenty six Murrah female river buffaloes, between 45 and 70 d post-partum, empty, multiparae, with an average live weight of 675 ± 56 kg, and average body condition of 3.5 points, in a 1 to 5 scale, were used to determine the concentrations of glucose, cholesterol, total protein and insulin-like growth factor type I(IGF-I) in the follicular fluid. The fluid was collected from dominant follicles, with diameters between 8 and 12 mm, by in vivo follicular aspiration. The oestrous cycle stage was not taken into account. The wave of follicular development was synchronized six days prior to the collection. Biochemical analyses of glucose and cholesterol were performed by the enzymatic colorimetric method with the utilization of commercial kits of Glicose (GOD-PAP) and Cholesterol (CHOD-PAP) (Kovalent), respectively. For the determination of total protein, the commercial kit total Protein (Kovalent), method Biuret, was employed. Readings were carried out through absorption spectrophotometry with visible light. Through the radioimmunoanalysis (RIA) technique the concentration of IGF-I was obtained using commercial kits of IRMA IGF-I (IMMUNOTECH). Descriptive statistics was used, by applying the PROC MEANS procedure of the SAS (2009) statistical package. Glucose concentrations (4.0 ± 0.75 mmol/L) and IGF-I (340 ± 129.83 ng/mL) showed higher values in female river buffaloes and dairy cows regarding those reported in other studies. However, cholesterol levels (0.51 ± 0.12 mmol/L) and total proteins (58.4 ± 4.43 g/L) were lower. Results indicate that there is a relationship between the concentration of biochemical indicators, the nutritional aspects, the diameter of the aspired follicles and the productive period. |
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Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis)Biochemical profileFollicular fluidPost-partumTwenty six Murrah female river buffaloes, between 45 and 70 d post-partum, empty, multiparae, with an average live weight of 675 ± 56 kg, and average body condition of 3.5 points, in a 1 to 5 scale, were used to determine the concentrations of glucose, cholesterol, total protein and insulin-like growth factor type I(IGF-I) in the follicular fluid. The fluid was collected from dominant follicles, with diameters between 8 and 12 mm, by in vivo follicular aspiration. The oestrous cycle stage was not taken into account. The wave of follicular development was synchronized six days prior to the collection. Biochemical analyses of glucose and cholesterol were performed by the enzymatic colorimetric method with the utilization of commercial kits of Glicose (GOD-PAP) and Cholesterol (CHOD-PAP) (Kovalent), respectively. For the determination of total protein, the commercial kit total Protein (Kovalent), method Biuret, was employed. Readings were carried out through absorption spectrophotometry with visible light. Through the radioimmunoanalysis (RIA) technique the concentration of IGF-I was obtained using commercial kits of IRMA IGF-I (IMMUNOTECH). Descriptive statistics was used, by applying the PROC MEANS procedure of the SAS (2009) statistical package. Glucose concentrations (4.0 ± 0.75 mmol/L) and IGF-I (340 ± 129.83 ng/mL) showed higher values in female river buffaloes and dairy cows regarding those reported in other studies. However, cholesterol levels (0.51 ± 0.12 mmol/L) and total proteins (58.4 ± 4.43 g/L) were lower. Results indicate that there is a relationship between the concentration of biochemical indicators, the nutritional aspects, the diameter of the aspired follicles and the productive period.Instituto de Ciencia Animal, Apartado Postal 24, San-José de las Lajas, MayabequeCampus de Botucatú Universidad Estadual Paulista Julio, Mesquita FilhoCampo Grande Universidad Federal de Mato Grosso, Mato GrossoCampus de Botucatú Universidad Estadual Paulista Julio, Mesquita FilhoInstituto de Ciencia AnimalUniversidade Estadual Paulista (UNESP)Universidad Federal de Mato GrossoGallego, C.Pardo, Margarita [UNESP]Camargos, AlineSousa, D. [UNESP]Lenz, María I.Ramos, A. A. [UNESP]Jorge, A. M.Da Cruz Ladim, Fernanda [UNESP]Oba, Eunice [UNESP]2022-04-29T08:49:23Z2022-04-29T08:49:23Z2012-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article365-368Cuban Journal of Agricultural Science, v. 46, n. 4, p. 365-368, 2012.0864-0408http://hdl.handle.net/11449/2321662-s2.0-84887951528Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCuban Journal of Agricultural Scienceinfo:eu-repo/semantics/openAccess2024-09-09T14:00:27Zoai:repositorio.unesp.br:11449/232166Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis) |
title |
Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis) |
spellingShingle |
Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis) Gallego, C. Biochemical profile Follicular fluid Post-partum |
title_short |
Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis) |
title_full |
Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis) |
title_fullStr |
Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis) |
title_full_unstemmed |
Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis) |
title_sort |
Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis) |
author |
Gallego, C. |
author_facet |
Gallego, C. Pardo, Margarita [UNESP] Camargos, Aline Sousa, D. [UNESP] Lenz, María I. Ramos, A. A. [UNESP] Jorge, A. M. Da Cruz Ladim, Fernanda [UNESP] Oba, Eunice [UNESP] |
author_role |
author |
author2 |
Pardo, Margarita [UNESP] Camargos, Aline Sousa, D. [UNESP] Lenz, María I. Ramos, A. A. [UNESP] Jorge, A. M. Da Cruz Ladim, Fernanda [UNESP] Oba, Eunice [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Ciencia Animal Universidade Estadual Paulista (UNESP) Universidad Federal de Mato Grosso |
dc.contributor.author.fl_str_mv |
Gallego, C. Pardo, Margarita [UNESP] Camargos, Aline Sousa, D. [UNESP] Lenz, María I. Ramos, A. A. [UNESP] Jorge, A. M. Da Cruz Ladim, Fernanda [UNESP] Oba, Eunice [UNESP] |
dc.subject.por.fl_str_mv |
Biochemical profile Follicular fluid Post-partum |
topic |
Biochemical profile Follicular fluid Post-partum |
description |
Twenty six Murrah female river buffaloes, between 45 and 70 d post-partum, empty, multiparae, with an average live weight of 675 ± 56 kg, and average body condition of 3.5 points, in a 1 to 5 scale, were used to determine the concentrations of glucose, cholesterol, total protein and insulin-like growth factor type I(IGF-I) in the follicular fluid. The fluid was collected from dominant follicles, with diameters between 8 and 12 mm, by in vivo follicular aspiration. The oestrous cycle stage was not taken into account. The wave of follicular development was synchronized six days prior to the collection. Biochemical analyses of glucose and cholesterol were performed by the enzymatic colorimetric method with the utilization of commercial kits of Glicose (GOD-PAP) and Cholesterol (CHOD-PAP) (Kovalent), respectively. For the determination of total protein, the commercial kit total Protein (Kovalent), method Biuret, was employed. Readings were carried out through absorption spectrophotometry with visible light. Through the radioimmunoanalysis (RIA) technique the concentration of IGF-I was obtained using commercial kits of IRMA IGF-I (IMMUNOTECH). Descriptive statistics was used, by applying the PROC MEANS procedure of the SAS (2009) statistical package. Glucose concentrations (4.0 ± 0.75 mmol/L) and IGF-I (340 ± 129.83 ng/mL) showed higher values in female river buffaloes and dairy cows regarding those reported in other studies. However, cholesterol levels (0.51 ± 0.12 mmol/L) and total proteins (58.4 ± 4.43 g/L) were lower. Results indicate that there is a relationship between the concentration of biochemical indicators, the nutritional aspects, the diameter of the aspired follicles and the productive period. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 2022-04-29T08:49:23Z 2022-04-29T08:49:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Cuban Journal of Agricultural Science, v. 46, n. 4, p. 365-368, 2012. 0864-0408 http://hdl.handle.net/11449/232166 2-s2.0-84887951528 |
identifier_str_mv |
Cuban Journal of Agricultural Science, v. 46, n. 4, p. 365-368, 2012. 0864-0408 2-s2.0-84887951528 |
url |
http://hdl.handle.net/11449/232166 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cuban Journal of Agricultural Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
365-368 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021308355837952 |