A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0010740 http://hdl.handle.net/11449/42330 |
Resumo: | Background: Germ cell transplantation results in fertile recipients and is the only available approach to functionally investigate the spermatogonial stem cell biology in mammals and probably in other vertebrates. In the current study, we describe a novel non-surgical methodology for efficient spermatogonial transplantation into the testes of adult tilapia (O. niloticus), in which endogenous spermatogenesis had been depleted with the cytostatic drug busulfan.Methodology/Principal Findings: Using two different tilapia strains, the production of fertile spermatozoa with donor characteristics was demonstrated in adult recipient, which also sired progeny with the donor genotype. Also, after cryopreservation tilapia spermatogonial cells were able to differentiate to spermatozoa in the testes of recipient fishes. These findings indicate that injecting germ cells directly into adult testis facilitates and enable fast generation of donor spermatogenesis and offspring compared to previously described methods.Conclusion: Therefore, a new suitable methodology for biotechnological investigations in aquaculture was established, with a high potential to improve the production of commercially valuable fish, generate transgenic animals and preserve endangered fish species. |
id |
UNSP_80b4fcb8549f537fa34506bc942580d6 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/42330 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) ModelBackground: Germ cell transplantation results in fertile recipients and is the only available approach to functionally investigate the spermatogonial stem cell biology in mammals and probably in other vertebrates. In the current study, we describe a novel non-surgical methodology for efficient spermatogonial transplantation into the testes of adult tilapia (O. niloticus), in which endogenous spermatogenesis had been depleted with the cytostatic drug busulfan.Methodology/Principal Findings: Using two different tilapia strains, the production of fertile spermatozoa with donor characteristics was demonstrated in adult recipient, which also sired progeny with the donor genotype. Also, after cryopreservation tilapia spermatogonial cells were able to differentiate to spermatozoa in the testes of recipient fishes. These findings indicate that injecting germ cells directly into adult testis facilitates and enable fast generation of donor spermatogenesis and offspring compared to previously described methods.Conclusion: Therefore, a new suitable methodology for biotechnological investigations in aquaculture was established, with a high potential to improve the production of commercially valuable fish, generate transgenic animals and preserve endangered fish species.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Minas Gerais, Dept Morphol, Lab Cellular Biol, Belo Horizonte, MG, BrazilSão Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, BrazilGM Alevinos LTDA, Contagem, MG, BrazilUniv Fed Minas Gerais, Dept Gen Biol, Lab Biotechnol & Mol Markers, Belo Horizonte, MG, BrazilSão Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, BrazilPublic Library ScienceUniversidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)GM Alevinos LTDALacerda, Samyra M. S. N.Batlouni, Sergio R. [UNESP]Costa, Guilherme M. J.Segatelli, Tania M.Quirino, Bruno R.Queiroz, Bruno M.Kalapothakis, EvanguedesFranca, Luiz R.2014-05-20T15:33:50Z2014-05-20T15:33:50Z2010-05-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1371/journal.pone.0010740Plos One. San Francisco: Public Library Science, v. 5, n. 5, p. 9, 2010.1932-6203http://hdl.handle.net/11449/4233010.1371/journal.pone.0010740WOS:000278017300013WOS000278017300013.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-04-09T15:36:43Zoai:repositorio.unesp.br:11449/42330Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:36:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model |
title |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model |
spellingShingle |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model Lacerda, Samyra M. S. N. |
title_short |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model |
title_full |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model |
title_fullStr |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model |
title_full_unstemmed |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model |
title_sort |
A New and Fast Technique to Generate Offspring after Germ Cells Transplantation in Adult Fish: The Nile Tilapia (Oreochromis niloticus) Model |
author |
Lacerda, Samyra M. S. N. |
author_facet |
Lacerda, Samyra M. S. N. Batlouni, Sergio R. [UNESP] Costa, Guilherme M. J. Segatelli, Tania M. Quirino, Bruno R. Queiroz, Bruno M. Kalapothakis, Evanguedes Franca, Luiz R. |
author_role |
author |
author2 |
Batlouni, Sergio R. [UNESP] Costa, Guilherme M. J. Segatelli, Tania M. Quirino, Bruno R. Queiroz, Bruno M. Kalapothakis, Evanguedes Franca, Luiz R. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Universidade Estadual Paulista (Unesp) GM Alevinos LTDA |
dc.contributor.author.fl_str_mv |
Lacerda, Samyra M. S. N. Batlouni, Sergio R. [UNESP] Costa, Guilherme M. J. Segatelli, Tania M. Quirino, Bruno R. Queiroz, Bruno M. Kalapothakis, Evanguedes Franca, Luiz R. |
description |
Background: Germ cell transplantation results in fertile recipients and is the only available approach to functionally investigate the spermatogonial stem cell biology in mammals and probably in other vertebrates. In the current study, we describe a novel non-surgical methodology for efficient spermatogonial transplantation into the testes of adult tilapia (O. niloticus), in which endogenous spermatogenesis had been depleted with the cytostatic drug busulfan.Methodology/Principal Findings: Using two different tilapia strains, the production of fertile spermatozoa with donor characteristics was demonstrated in adult recipient, which also sired progeny with the donor genotype. Also, after cryopreservation tilapia spermatogonial cells were able to differentiate to spermatozoa in the testes of recipient fishes. These findings indicate that injecting germ cells directly into adult testis facilitates and enable fast generation of donor spermatogenesis and offspring compared to previously described methods.Conclusion: Therefore, a new suitable methodology for biotechnological investigations in aquaculture was established, with a high potential to improve the production of commercially valuable fish, generate transgenic animals and preserve endangered fish species. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-05-20 2014-05-20T15:33:50Z 2014-05-20T15:33:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0010740 Plos One. San Francisco: Public Library Science, v. 5, n. 5, p. 9, 2010. 1932-6203 http://hdl.handle.net/11449/42330 10.1371/journal.pone.0010740 WOS:000278017300013 WOS000278017300013.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0010740 http://hdl.handle.net/11449/42330 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 5, n. 5, p. 9, 2010. 1932-6203 10.1371/journal.pone.0010740 WOS:000278017300013 WOS000278017300013.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649901629800448 |