Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats

Detalhes bibliográficos
Autor(a) principal: Oliveira, Priscila Ramos de
Data de Publicação: 2020
Outros Autores: Oliveira, Priscilla Bianca de, Rossignoli, Patrícia de Souza [UNESP], Spadella, Maria Angélica, Chies, Agnaldo Bruno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1113/EP088139
http://hdl.handle.net/11449/200100
Resumo: New Findings: What is the central question of this study? What are the effects of exercise on Ang II-induced vasoconstriction in aortas of normotensive rats and how do these effects occur in two-kidney–one-clip hypertensive animals? What is the main finding and its importance? In two-kidney rats, exercise training improves the Ang II-induced vasoconstriction by endothelium-derived NO released through AT2R activation. This effect of exercise training on the Ang II-induced vasoconstriction is blunted in two-kidney–one-clip hypertensive animals, possibly as a consequence of oxidative stress. Abstract: This study investigated the effects of both acute exercise and training on the Ang II-induced vasoconstriction in aorta of normotensive (two-kidney; 2K) and two-kidney–one-clip (2K1C) hypertensive rats, focusing on endothelial mechanisms related to nitric oxide (NO) and prostanoids. Aorta rings of 2K and 2K1C male Wistar rats, sedentary and trained, killed at rest and after acute exercise, were challenged with Ang II in either the absence or the presence of PD 123,319, a selective angiotensin receptor subtype 2 (AT2R) antagonist; Nω-nitro-l-arginine methyl ester (l-NAME), a non-selective inhibitor of nitric oxide synthase; indomethacin, a non-selective inhibitor of cyclooxygenase; or Tiron, an analogue of superoxide dismutase. Aortas of sedentary and trained animals studied at rest were also submitted to histomorphometric analysis. Exercise training reduced the Ang II-induced vasoconstriction in aorta of 2K but not of 2K1C animals. This reduction of Ang II response in aortas of 2K animals was not found after endothelial removal or treatment with PD 123,319 or l-NAME. These results suggest that exercise training improves the modulation of Ang II-induced vasoconstriction in aorta of 2K animals, by endothelium-derived NO released due to the activation of AT2R. No exercise-induced change of Ang II response occurred in 2K1C animals, except in the presence of Tiron, which was evidence for reduction of such responses only in resting trained 2K1C animals. In 2K1C animals, NO modulation of Ang II-induced vasoconstriction might be suppressed by local oxidative stress. Moreover, exercise training slightly reduced the media layer thickness in the aortas of the 2K1C, but not 2K animals, which may indicate cardiovascular protection of these animals.
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spelling Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive ratsangiotensin IIaortaexerciseNew Findings: What is the central question of this study? What are the effects of exercise on Ang II-induced vasoconstriction in aortas of normotensive rats and how do these effects occur in two-kidney–one-clip hypertensive animals? What is the main finding and its importance? In two-kidney rats, exercise training improves the Ang II-induced vasoconstriction by endothelium-derived NO released through AT2R activation. This effect of exercise training on the Ang II-induced vasoconstriction is blunted in two-kidney–one-clip hypertensive animals, possibly as a consequence of oxidative stress. Abstract: This study investigated the effects of both acute exercise and training on the Ang II-induced vasoconstriction in aorta of normotensive (two-kidney; 2K) and two-kidney–one-clip (2K1C) hypertensive rats, focusing on endothelial mechanisms related to nitric oxide (NO) and prostanoids. Aorta rings of 2K and 2K1C male Wistar rats, sedentary and trained, killed at rest and after acute exercise, were challenged with Ang II in either the absence or the presence of PD 123,319, a selective angiotensin receptor subtype 2 (AT2R) antagonist; Nω-nitro-l-arginine methyl ester (l-NAME), a non-selective inhibitor of nitric oxide synthase; indomethacin, a non-selective inhibitor of cyclooxygenase; or Tiron, an analogue of superoxide dismutase. Aortas of sedentary and trained animals studied at rest were also submitted to histomorphometric analysis. Exercise training reduced the Ang II-induced vasoconstriction in aorta of 2K but not of 2K1C animals. This reduction of Ang II response in aortas of 2K animals was not found after endothelial removal or treatment with PD 123,319 or l-NAME. These results suggest that exercise training improves the modulation of Ang II-induced vasoconstriction in aorta of 2K animals, by endothelium-derived NO released due to the activation of AT2R. No exercise-induced change of Ang II response occurred in 2K1C animals, except in the presence of Tiron, which was evidence for reduction of such responses only in resting trained 2K1C animals. In 2K1C animals, NO modulation of Ang II-induced vasoconstriction might be suppressed by local oxidative stress. Moreover, exercise training slightly reduced the media layer thickness in the aortas of the 2K1C, but not 2K animals, which may indicate cardiovascular protection of these animals.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Pharmacology Marília Medical SchoolDepartment of Physiotherapy and Occupational Therapy São Paulo State University (UNESP)Human Embryology Laboratory Marília Medical SchoolDepartment of Physiotherapy and Occupational Therapy São Paulo State University (UNESP)CAPES: 1576988FAPESP: 2013/22655-9Marília Medical SchoolUniversidade Estadual Paulista (Unesp)Oliveira, Priscila Ramos deOliveira, Priscilla Bianca deRossignoli, Patrícia de Souza [UNESP]Spadella, Maria AngélicaChies, Agnaldo Bruno2020-12-12T01:57:45Z2020-12-12T01:57:45Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article732-742http://dx.doi.org/10.1113/EP088139Experimental Physiology, v. 105, n. 4, p. 732-742, 2020.1469-445X0958-0670http://hdl.handle.net/11449/20010010.1113/EP0881392-s2.0-8507988618726229754535630850000-0001-6946-1145Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental Physiologyinfo:eu-repo/semantics/openAccess2024-08-09T15:17:25Zoai:repositorio.unesp.br:11449/200100Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-09T15:17:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats
title Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats
spellingShingle Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats
Oliveira, Priscila Ramos de
angiotensin II
aorta
exercise
title_short Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats
title_full Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats
title_fullStr Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats
title_full_unstemmed Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats
title_sort Exercise training attenuates angiotensin II-induced vasoconstriction in the aorta of normotensive but not hypertensive rats
author Oliveira, Priscila Ramos de
author_facet Oliveira, Priscila Ramos de
Oliveira, Priscilla Bianca de
Rossignoli, Patrícia de Souza [UNESP]
Spadella, Maria Angélica
Chies, Agnaldo Bruno
author_role author
author2 Oliveira, Priscilla Bianca de
Rossignoli, Patrícia de Souza [UNESP]
Spadella, Maria Angélica
Chies, Agnaldo Bruno
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Marília Medical School
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Oliveira, Priscila Ramos de
Oliveira, Priscilla Bianca de
Rossignoli, Patrícia de Souza [UNESP]
Spadella, Maria Angélica
Chies, Agnaldo Bruno
dc.subject.por.fl_str_mv angiotensin II
aorta
exercise
topic angiotensin II
aorta
exercise
description New Findings: What is the central question of this study? What are the effects of exercise on Ang II-induced vasoconstriction in aortas of normotensive rats and how do these effects occur in two-kidney–one-clip hypertensive animals? What is the main finding and its importance? In two-kidney rats, exercise training improves the Ang II-induced vasoconstriction by endothelium-derived NO released through AT2R activation. This effect of exercise training on the Ang II-induced vasoconstriction is blunted in two-kidney–one-clip hypertensive animals, possibly as a consequence of oxidative stress. Abstract: This study investigated the effects of both acute exercise and training on the Ang II-induced vasoconstriction in aorta of normotensive (two-kidney; 2K) and two-kidney–one-clip (2K1C) hypertensive rats, focusing on endothelial mechanisms related to nitric oxide (NO) and prostanoids. Aorta rings of 2K and 2K1C male Wistar rats, sedentary and trained, killed at rest and after acute exercise, were challenged with Ang II in either the absence or the presence of PD 123,319, a selective angiotensin receptor subtype 2 (AT2R) antagonist; Nω-nitro-l-arginine methyl ester (l-NAME), a non-selective inhibitor of nitric oxide synthase; indomethacin, a non-selective inhibitor of cyclooxygenase; or Tiron, an analogue of superoxide dismutase. Aortas of sedentary and trained animals studied at rest were also submitted to histomorphometric analysis. Exercise training reduced the Ang II-induced vasoconstriction in aorta of 2K but not of 2K1C animals. This reduction of Ang II response in aortas of 2K animals was not found after endothelial removal or treatment with PD 123,319 or l-NAME. These results suggest that exercise training improves the modulation of Ang II-induced vasoconstriction in aorta of 2K animals, by endothelium-derived NO released due to the activation of AT2R. No exercise-induced change of Ang II response occurred in 2K1C animals, except in the presence of Tiron, which was evidence for reduction of such responses only in resting trained 2K1C animals. In 2K1C animals, NO modulation of Ang II-induced vasoconstriction might be suppressed by local oxidative stress. Moreover, exercise training slightly reduced the media layer thickness in the aortas of the 2K1C, but not 2K animals, which may indicate cardiovascular protection of these animals.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:57:45Z
2020-12-12T01:57:45Z
2020-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1113/EP088139
Experimental Physiology, v. 105, n. 4, p. 732-742, 2020.
1469-445X
0958-0670
http://hdl.handle.net/11449/200100
10.1113/EP088139
2-s2.0-85079886187
2622975453563085
0000-0001-6946-1145
url http://dx.doi.org/10.1113/EP088139
http://hdl.handle.net/11449/200100
identifier_str_mv Experimental Physiology, v. 105, n. 4, p. 732-742, 2020.
1469-445X
0958-0670
10.1113/EP088139
2-s2.0-85079886187
2622975453563085
0000-0001-6946-1145
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Experimental Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 732-742
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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